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Cellular populations are typically heterogenous collections of cells at different points in their respective cell cycles, each with a cell cycle time that varies from individual to individual. As a result, true single-cell behavior,…

Quantitative Methods · Quantitative Biology 2013-07-02 Marisa C. Eisenberg , Joshua N. Ash , Dan Siegal-Gaskins

Cell heterogeneity and the inherent complexity due to the interplay of multiple molecular processes within the cell pose difficult challenges for current single-cell biology. We introduce an approach that identifies a disease phenotype from…

Modern high-throughput sequencing technologies have enabled us to profile multiple molecular modalities from the same single cell, providing unprecedented opportunities to assay celluar heterogeneity from multiple biological layers.…

Machine Learning · Statistics 2022-05-20 Pengcheng Zeng , Zhixiang Lin

The study of expression Quantitative Trait Loci (eQTL) is an important problem in genomics and biomedicine. While detection (testing) of eQTL associations has been widely studied, less work has been devoted to the estimation of eQTL effect…

Methodology · Statistics 2017-09-08 John Palowitch , Andrey Shabalin , Yihui Zhou , Andrew B. Nobel , Fred A. Wright

Single-cell sequencing technologies have significantly advanced molecular and cellular biology, offering unprecedented insights into cellular heterogeneity by allowing for the measurement of gene expression at an individual cell level.…

Methodology · Statistics 2024-03-26 Junsouk Choi , Hee Cheol Chung , Irina Gaynanova , Yang Ni

By summarizing longer consumer health questions into shorter and essential ones, medical question-answering systems can more accurately understand consumer intentions and retrieve suitable answers. However, medical question summarization is…

Computation and Language · Computer Science 2025-10-01 Wenpeng Lu , Sibo Wei , Xueping Peng , Yi-fei Wang , Usman Naseem , Shoujin Wang

Single-cell transcriptomics, epigenomics, and other 'omics applied at single-cell resolution can significantly advance hypotheses and understanding of glial biology. Omics technologies are revealing a large and growing number of new glial…

Quantitative Methods · Quantitative Biology 2024-08-14 Katherine E. Prater , Kevin Z. Lin

Supervised Causal Learning (SCL) has shown promise in causal discovery by framing it as a supervised learning problem. However, it suffers from significant out-of-distribution generalization challenges. We reveal three limitations of…

Machine Learning · Computer Science 2026-05-29 Zizhen Deng , Jiaru Zhang , Rui Ding , Huang Bojun , Jinzhuo Wang , Qiang Fu , Shi Han , Dongmei Zhang

Single-cell RNA sequencing (scRNA-seq) has the potential to provide powerful, high-resolution signatures to inform disease prognosis and precision medicine. This paper takes an important first step towards this goal by developing an…

Quantitative Methods · Quantitative Biology 2021-10-15 Bryan He , Matthew Thomson , Meena Subramaniam , Richard Perez , Chun Jimmie Ye , James Zou

Designing regulatory DNA sequences that achieve precise cell-type-specific gene expression is crucial for advancements in synthetic biology, gene therapy and precision medicine. Although transformer-based language models (LMs) can…

Machine Learning · Computer Science 2025-05-28 Xingyu Chen , Shihao Ma , Runsheng Lin , Jiecong Lin , Bo Wang

Self-supervised learning (SSL) has proven to be a powerful approach for extracting biologically meaningful representations from single-cell data. To advance our understanding of SSL methods applied to single-cell data, we present…

Quantitative Methods · Quantitative Biology 2025-06-13 Olga Ovcharenko , Florian Barkmann , Philip Toma , Imant Daunhawer , Julia Vogt , Sebastian Schelter , Valentina Boeva

Whole and targeted sequencing of human genomes is a promising, increasingly feasible tool for discovering genetic contributions to risk of complex diseases. A key step is calling an individual's genotype from the multiple aligned short read…

Applications · Statistics 2012-06-29 Baiyu Zhou , Alice S. Whittemore

We present a novel method for automated identification of putative cell types from single-cell RNA-seq (scRNA-seq) data. By iteratively applying a machine learning approach to an initial clustering of gene expression profiles of a given set…

Quantitative Methods · Quantitative Biology 2020-04-22 Zhichao Miao , Pablo Moreno , Ni Huang , Irene Papatheodorou , Alvis Brazma , Sarah A Teichmann

Transcriptome foundation models TFMs hold great promises of deciphering the transcriptomic language that dictate diverse cell functions by self-supervised learning on large-scale single-cell gene expression data, and ultimately unraveling…

Machine Learning · Computer Science 2025-03-03 Xinyu Yuan , Zhihao Zhan , Zuobai Zhang , Manqi Zhou , Jianan Zhao , Boyu Han , Yue Li , Jian Tang

The discovery of genomic polymorphisms influencing gene expression (also known as expression quantitative trait loci or eQTLs) can be formulated as a sparse Bayesian multivariate/multiple regression problem. An important aspect in the…

Genomics · Quantitative Biology 2019-06-13 Dimitrios V Vavoulis

Single-cell RNA-seq data allow the quantification of cell type differences across a growing set of biological contexts. However, pinpointing a small subset of genomic features explaining this variability can be ill-defined and…

Machine Learning · Statistics 2022-07-29 Nabeel Sarwar , Wilson Gregory , George A Kevrekidis , Soledad Villar , Bianca Dumitrascu

Single-cell RNA sequencing (scRNA-seq) has transformed our ability to explore biological systems. Nevertheless, proficient expertise is essential for handling and interpreting the data. In this paper, we present scX, an R package built on…

Single-cell RNA sequencing (scRNA-seq) determines RNA expression at single-cell resolution. It provides a powerful tool for studying immunity, regulation, and other life activities of cells. However, due to the limitations of the sequencing…

Genomics · Quantitative Biology 2024-02-16 Linfeng Jiang , Yuan Zhu

Identifying genes associated with diseases is crucial to understanding disease mechanisms and developing therapies. However, identification of individual genes associated with a disease often needs to be supplemented with clustering…

Genomics · Quantitative Biology 2026-04-30 Brayan Gutierrez , Rinki Ratnapriya , Arko Barman

Many DNA sequence variants influence phenotypes by altering gene expression. Our understanding of these variants is limited by sample sizes of current studies and by measurements of mRNA rather than protein abundance. We developed a…

Genomics · Quantitative Biology 2015-06-16 Frank W. Albert , Sebastian Treusch , Arthur H. Shockley , Joshua S. Bloom , Leonid Kruglyak