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Since the advent of optogenetics, technology development has focused on new methods to optically interact with single nervous cells. This gave rise to the field of photonic neural interfaces, intended as the set of technologies that can…

Cell Behavior · Quantitative Biology 2019-07-15 Filippo Pisano , Marco Pisanello , Massimo De Vittorio , Ferruccio Pisanello

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly…

Image and Video Processing · Electrical Eng. & Systems 2021-10-22 Daniele Ancora , Gianluca Valentini , Antonio Pifferi , Andrea Bassi

We employed an electrically-driven polarization controller to implement anisotropic depolarizing quantum channels for the polarization state of single photons. The channels were characterized by means of ancilla-assisted quantum process…

Quantum Physics · Physics 2010-07-22 Michal Karpinski , Czeslaw Radzewicz , Konrad Banaszek

Imaging dynamics of cellular morphogenesis with high spatial-temporal resolution in 3D is challenging, due to the low spatial resolution along the optical axis and photo-toxicity. However, some cellular structures are planar and hence 2D…

Quantitative Methods · Quantitative Biology 2020-07-14 Alka Bhat , Linjie Lu , Chen-Ho Wang , Simon Lo Vecchio , Riccardo Maraspini , Alf Honigmann , Daniel Riveline

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

The acquisition of multi-view information of cells is essential for accurate 3D reconstruction of their structures. Rotational manipulation of cells has emerged as an effective technique for obtaining such data. However, most reported…

Optics · Physics 2025-06-10 Yiwei Pan , Yijing Wu , Ziqiang Wang , Yinmei Li , Lei Gong

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

We present a new interferometric imaging approach that allows for multiple-depth imaging in a single acquisition, using off-axis low-coherence holographic multiplexing. This technique enables sectioned imaging of multiple slices within a…

Optics · Physics 2019-03-29 Lauren Wolbromsky , Nir A. Turko , Natan T. Shaked

Multichannel, infinite-conjugate optical systems easily allow implementation of multiple image paths and imaging modes into a single microscope. Traditional optical alignment methods which rely on additional hardware are not always simple…

Optics · Physics 2024-07-16 Gemma S. Cairns , Brian R. Patton

Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…

Image and Video Processing · Electrical Eng. & Systems 2026-01-28 Chenwei Wang , Zhaoke Huang , Zelin Li , Wenqi Zhu

Non-invasive and single-shot holographic imaging through complex media is technically challenging due to random light scattering which significantly scrambles optical information. Recently, several methods have been presented to address…

Combining optical tweezers with fluorescence microscopy is a powerful tool for single-cell analysis, playing a pivotal role in disease diagnosis, cell sorting, and the investigation of cellular dynamics. However, fluorescence detection…

Optics · Physics 2026-04-07 Jun Yin , Sanyou Chen , Yihao Yan , Mengqi Wang , Ya Wang , Yiheng Lin , Qi Zhang , Fazhan Shi

Interferometric imaging now achieves angular resolutions as fine as 10 microarcsec, probing scales that are inaccessible to single telescopes. Traditional synthesis imaging methods require calibrated visibilities; however, interferometric…

Instrumentation and Methods for Astrophysics · Physics 2018-04-25 Andrew A. Chael , Michael D. Johnson , Katherine L. Bouman , Lindy L. Blackburn , Kazunori Akiyama , Ramesh Narayan

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Optical and optoelectronic techniques for micro- and nano-object manipulation are becoming essential tools in nano- and bio-technology. A remarkable optoelectronic technique that has experimented a strong development in the last few years…

Clinical routine and retrospective cohorts commonly include multi-parametric Magnetic Resonance Imaging; however, they are mostly acquired in different anisotropic 2D views due to signal-to-noise-ratio and scan-time constraints. Thus…

Investigating the cellular internalization pathways of single molecules or single nano-objects is important to understanding cell-matter interactions and to applications in drug delivery and discovery. Imaging and tracking the motion of…

Biological Physics · Physics 2015-06-04 Guosong Hong , Justin Z. Wu , Joshua T. Robinson , Hailiang Wang , Bo Zhang , Hongjie Dai

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes /…

Optics · Physics 2020-10-29 Yujia Xue , Ian G. Davison , David A. Boas , Lei Tian