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Calcium imaging has emerged as a workhorse method in neuroscience to investigate patterns of neuronal activity. Instrumentation to acquire calcium imaging movies has rapidly progressed and has become standard across labs. Still, algorithms…

Quantitative Methods · Quantitative Biology 2019-06-03 Quico Spaen , Dorit S. Hochbaum , Roberto Asín-Achá

In the past few years, new technologies in the field of neuroscience have made it possible to simultaneously image activity in large populations of neurons at cellular resolution in behaving animals. In mid-2016, a huge repository of this…

Applications · Statistics 2017-03-22 Ashley Petersen , Noah Simon , Daniela Witten

Calcium imaging is a technique for observing neuron activity as a series of images showing indicator fluorescence over time. Manually segmenting neurons is time-consuming, leading to research on automated calcium imaging segmentation…

Computer Vision and Pattern Recognition · Computer Science 2017-07-21 Aleksander Klibisz , Derek Rose , Matthew Eicholtz , Jay Blundon , Stanislav Zakharenko

Calcium imaging has become a fundamental neural imaging technique, aiming to recover the individual activity of hundreds of neurons in a cortical region. Current methods (mostly matrix factorization) are aimed at detecting neurons in the…

Applications · Statistics 2019-02-11 Gal Mishne , Adam S. Charles

Understanding how neurons coordinate their activity is a fundamental question in neuroscience, with implications for learning, memory, and neurological disorders. Calcium imaging has emerged as a powerful method to observe large-scale…

Methodology · Statistics 2026-03-03 Laura D'Angelo , Francesco Denti , Antonio Canale , Michele Guindani

Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important. Here we apply a…

Neurons and Cognition · Quantitative Biology 2016-12-23 Noah J. Apthorpe , Alexander J. Riordan , Rob E. Aguilar , Jan Homann , Yi Gu , David W. Tank , H. Sebastian Seung

Accurate cell instance segmentation is foundational for digital pathology analysis. Existing methods based on contour detection and distance mapping still face significant challenges in processing complex and dense cellular regions. Graph…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Rui Sun , Yiwen Yang , Kaiyu Guo , Chen Jiang , Dongli Xu , Zhaonan Liu , Tan Pan , Limei Han , Xue Jiang , Wu Wei , Yuan Cheng

Deep learning has proven to be more effective than other methods in medical image analysis, including the seemingly simple but challenging task of segmenting individual cells, an essential step for many biological studies. Comparative…

Image and Video Processing · Electrical Eng. & Systems 2023-11-07 Valentina Vadori , Antonella Peruffo , Jean-Marie Graïc , Livio Finos , Livio Corain , Enrico Grisan

Neurons encode information about the environment through their activity. As animals explore the environment, neurons rapidly acquire selectivity for distinct features of the external world; characterizing how these selectivity patterns…

We present a structured matrix factorization approach to analyzing calcium imaging recordings of large neuronal ensembles. Our goal is to simultaneously identify the locations of the neurons, demix spatially overlapping components, and…

Neurons and Cognition · Quantitative Biology 2014-09-11 Eftychios A. Pnevmatikakis , Yuanjun Gao , Daniel Soudry , David Pfau , Clay Lacefield , Kira Poskanzer , Randy Bruno , Rafael Yuste , Liam Paninski

Calcium imaging allows for the parallel measurement of large neuronal populations in a spatially resolved and minimally invasive manner, and has become a gold-standard for neuronal functionality. While deep generative models have been…

Neurons and Cognition · Quantitative Biology 2025-10-02 Berta Ros , Mireia Olives-Verger , Caterina Fuses , Josep M Canals , Jordi Soriano , Jordi Abante

We present an interactive approach to train a deep neural network pixel classifier for the segmentation of neuronal structures. An interactive training scheme reduces the extremely tedious manual annotation task that is typically required…

Computer Vision and Pattern Recognition · Computer Science 2016-10-31 Felix Gonda , Verena Kaynig , Ray Thouis , Daniel Haehn , Jeff Lichtman , Toufiq Parag , Hanspeter Pfister

In vivo calcium imaging through microscopes has enabled deep brain imaging of previously inaccessible neuronal populations within the brains of freely moving subjects. However, microendoscopic data suffer from high levels of background…

Every year millions of people die due to disease of Cancer. Due to its invasive nature it is very complex to cure even in primary stages. Hence, only method to survive this disease completely is via forecasting by analyzing the early…

Computer Vision and Pattern Recognition · Computer Science 2018-08-24 Shivam Singh , Stuti Pathak

Accurate cell segmentation is critical for biological and medical imaging studies. Although recent deep learning models have advanced this task, most methods are limited to generic cell segmentation, lacking the ability to differentiate…

Computer Vision and Pattern Recognition · Computer Science 2026-03-17 Bisheng Wang , Jaime S. Cardoso , Lin Wu

Cell individualization has a vital role in digital pathology image analysis. Deep Learning is considered as an efficient tool for instance segmentation tasks, including cell individualization. However, the precision of the Deep Learning…

Image and Video Processing · Electrical Eng. & Systems 2022-08-09 Huaqian Wu , Nicolas Souedet , Zhenzhen You , Caroline Jan , Cédric Clouchoux , Thierry Delzescaux

Deep learning models have shown promising performance for cell nucleus segmentation in the field of pathology image analysis. However, training a robust model from multiple domains remains a great challenge for cell nucleus segmentation.…

Image and Video Processing · Electrical Eng. & Systems 2024-03-12 Dawei Fan , Yifan Gao , Jiaming Yu , Yanping Chen , Wencheng Li , Chuancong Lin , Kaibin Li , Changcai Yang , Riqing Chen , Lifang Wei

Coronary artery calcium (CAC) is a significant marker of atherosclerosis and cardiovascular events. In this work we present a system for the automatic quantification of calcium score in ECG-triggered non-contrast enhanced cardiac computed…

Computer Vision and Pattern Recognition · Computer Science 2017-10-10 G. Santini , D. Della Latta , N. Martini , G. Valvano , A. Gori , A. Ripoli , C. L. Susini , L. Landini , D. Chiappino

We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

In this work, we propose a simple yet effective solution to the problem of connectome inference in calcium imaging data. The proposed algorithm consists of two steps. First, processing the raw signals to detect neural peak activities.…

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