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Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Automatic cell segmentation in microscopy images works well with the support of deep neural networks trained with full supervision. Collecting and annotating images, though, is not a sustainable solution for every new microscopy database…

Computer Vision and Pattern Recognition · Computer Science 2020-07-06 Youssef Dawoud , Julia Hornauer , Gustavo Carneiro , Vasileios Belagiannis

Deep learning algorithms have become the golden standard for segmentation of medical imaging data. In most works, the variability and heterogeneity of real clinical data is acknowledged to still be a problem. One way to automatically…

Image and Video Processing · Electrical Eng. & Systems 2022-02-25 Arkadiy Dushatskiy , Gerry Lowe , Peter A. N. Bosman , Tanja Alderliesten

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be made in tools that rely on classical methods for segmentation,…

Quantitative Methods · Quantitative Biology 2024-03-15 Nodar Gogoberidze , Beth A. Cimini

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Biological membranes are one of the most basic structures and regions of interest in cell biology. In the study of membranes, segment extraction is a well-known and difficult problem because of impeding noise, directional and thickness…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jonas De Vylder , Jan Aelterman , Yvan Saeys , Wilfried Philips

The emerging field of connectomics aims to unlock the mysteries of the brain by understanding the connectivity between neurons. To map this connectivity, we acquire thousands of electron microscopy (EM) images with nanometer-scale…

Quantitative Methods · Quantitative Biology 2016-04-04 Stephen M. Plaza , Stuart E. Berg

One of the major difficulties in medical image segmentation is the high variability of these images, which is caused by their origin (multi-centre), the acquisition protocols (multi-parametric), as well as the variability of human anatomy,…

Image and Video Processing · Electrical Eng. & Systems 2022-11-09 Jhon Jairo Saenz-Gamboa , Julio Domenech , Antonio Alonso-Manjarrés , Jon A. Gómez , Maria de la Iglesia-Vayá

The need for labour intensive pixel-wise annotation is a major limitation of many fully supervised learning methods for segmenting bioimages that can contain numerous object instances with thin separations. In this paper, we introduce a…

Computer Vision and Pattern Recognition · Computer Science 2020-09-11 Rihuan Ke , Aurélie Bugeau , Nicolas Papadakis , Peter Schuetz , Carola-Bibiane Schönlieb

Cell segmentation is a major bottleneck in extracting quantitative single-cell information from microscopy data. The challenge is exasperated in the setting of microstructured environments. While deep learning approaches have proven useful…

Quantitative Methods · Quantitative Biology 2021-01-08 Tim Prangemeier , Christian Wildner , André O. Françani , Christoph Reich , Heinz Koeppl

Medical image segmentation is a fundamental and critical step in many image-guided clinical approaches. Recent success of deep learning-based segmentation methods usually relies on a large amount of labeled data, which is particularly…

Computer Vision and Pattern Recognition · Computer Science 2023-11-15 Rushi Jiao , Yichi Zhang , Le Ding , Rong Cai , Jicong Zhang

Detecting and segmenting individual cells from microscopy images is critical to various life science applications. Traditional cell segmentation tools are often ill-suited for applications in brightfield microscopy due to poor contrast and…

Image and Video Processing · Electrical Eng. & Systems 2020-05-20 Rituparna Sarkar , Suvadip Mukherjee , Elisabeth Labruyère , Jean-Christophe Olivo-Marin

Identifying complex neural circuitry from electron microscopic (EM) images may help unlock the mysteries of the brain. However, identifying this circuitry requires time-consuming, manual tracing (proofreading) due to the size and intricacy…

Quantitative Methods · Quantitative Biology 2014-09-04 Stephen M. Plaza

The prospect of neural reconstruction from Electron Microscopy (EM) images has been elucidated by the automatic segmentation algorithms. Although segmentation algorithms eliminate the necessity of tracing the neurons by hand, significant…

Computer Vision and Pattern Recognition · Computer Science 2015-09-29 Toufiq Parag

Image segmentation is an important task in many medical applications. Methods based on convolutional neural networks attain state-of-the-art accuracy; however, they typically rely on supervised training with large labeled datasets. Labeling…

Computer Vision and Pattern Recognition · Computer Science 2019-04-09 Amy Zhao , Guha Balakrishnan , Frédo Durand , John V. Guttag , Adrian V. Dalca

Medical image segmentation has advanced rapidly over the past two decades, largely driven by deep learning, which has enabled accurate and efficient delineation of cells, tissues, organs, and pathologies across diverse imaging modalities.…

Image and Video Processing · Electrical Eng. & Systems 2025-08-29 Guoping Xu , Jayaram K. Udupa , Jax Luo , Songlin Zhao , Yajun Yu , Scott B. Raymond , Hao Peng , Lipeng Ning , Yogesh Rathi , Wei Liu , You Zhang

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Deep learning techniques have shown great potential in medical image processing, particularly through accurate and reliable image segmentation on magnetic resonance imaging (MRI) scans or computed tomography (CT) scans, which allow the…

Image and Video Processing · Electrical Eng. & Systems 2022-05-10 Yang Liu , Ersi Zhang , Lulu Xu , Chufan Xiao , Xiaoyun Zhong , Lijin Lian , Fang Li , Bin Jiang , Yuhan Dong , Lan Ma , Qiming Huang , Ming Xu , Yongbing Zhang , Dongmei Yu , Chenggang Yan , Peiwu Qin

Every year millions of people die due to disease of Cancer. Due to its invasive nature it is very complex to cure even in primary stages. Hence, only method to survive this disease completely is via forecasting by analyzing the early…

Computer Vision and Pattern Recognition · Computer Science 2018-08-24 Shivam Singh , Stuti Pathak

Despite the recent success of deep learning methods at achieving new state-of-the-art accuracy for medical image segmentation, some major limitations are still restricting their deployment into clinics. One major limitation of deep…

Image and Video Processing · Electrical Eng. & Systems 2023-05-30 Lucas Fidon
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