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Three-dimensional (3D) high-resolution imaging is essential in microscopy, yet light scattering poses significant challenges in achieving it. Here, we present an approach to holographic imaging of spatially incoherent objects through…

Optics · Physics 2025-02-04 YoonSeok Baek , Hilton B. de Aguiar , Sylvain Gigan

We extend our previously proposed image reconstruction method, which allows confocal microscopes to capture periodically moving objects at frequencies beyond their frame rates, to three-dimensional and two-dimensional wide-field imaging.…

Optics · Physics 2026-03-03 Kanta Adachi , Soyoka Hemmi , Nobutomo Nakamura

A robust method and strategy for efficient full field-ofview and depth separation optical imaging through scattering media regardless of the three-dimensional (3D) optical memory effect are proposed. In this method, the problem of imaging…

Optics · Physics 2020-01-17 Wei Li , Jietao Liu , Shunfu He , Lixian Liu , Xiaopeng Shao

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

Medical Physics · Physics 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

An holographic microscopy reconstruction method compatible with high numerical aperture microscope objective (MO) up to NA=1.4 is proposed. After off axis and reference field curvature corrections, and after selection of the +1 grating…

Optics · Physics 2015-11-16 Nicolas Verrier , Dario Donnarumma , Gilles Tessier , Michel Gross

Optical sensing is one of the key-enablers of modern diagnostics. Especially label-free imaging modalities hold great promise as they eliminate labeling procedures prior to analysis. However, scattering signals of nanometric particles scale…

Optics · Physics 2021-02-03 Unai Ortiz-Orruño , Ala Jo , Hakho Lee , Niek F. van Hulst , Matz Liebel

Diffusion models (DMs) have recently been introduced in image deblurring and exhibited promising performance, particularly in terms of details reconstruction. However, the diffusion model requires a large number of inference iterations to…

Computer Vision and Pattern Recognition · Computer Science 2023-09-26 Zheng Chen , Yulun Zhang , Ding Liu , Bin Xia , Jinjin Gu , Linghe Kong , Xin Yuan

We present a new, high-speed technique to track the three-dimensional translation and rotation of non-spherical colloidal particles. We capture digital holograms of micrometer-scale silica rods and sub-micrometer-scale Janus particles…

We consider a general method of deprojecting 2D images to reconstruct the 3D structure of the projected object, assuming axial symmetry. The method consists of the application of the Fourier Slice Theorem to the general case where the axis…

Astrophysics · Physics 2009-10-30 S. Zaroubi , G. Squires , Y. Hoffman , J. Silk

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Three-dimensional phase contrast imaging of multiply-scattering samples in X-ray and electron microscopy is extremely challenging, due to small numerical apertures, the unavailability of wavefront shaping optics, and the highly nonlinear…

Computational Physics · Physics 2021-06-02 Philipp M Pelz , Hamish G Brown , Jim Ciston , Scott D Findlay , Yaqian Zhang , Mary Scott , Colin Ophus

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

We report on image processing techniques and experimental procedures to determine the lattice-site positions of single atoms in an optical lattice with high reliability, even for limited acquisition time or optical resolution. Determining…

Coherent X-ray diffraction microscopy is a method of imaging non-periodic isolated objects at resolutions only limited, in principle, by the largest scattering angles recorded. We demonstrate X-ray diffraction imaging with high resolution…

X-ray fluorescence holography (XFH) is a method for obtaining diffraction-limited images of the local atomic structure around a given type of emitter. The reconstructed wave-field represents a distorted image of the scatterer electron…

Condensed Matter · Physics 2009-09-29 S. Marchesini , C. S. Fadley , F. J. Garcia de Abajo

This paper proposes a particle volume reconstruction directly from an in-line hologram using a deep neural network. Digital holographic volume reconstruction conventionally uses multiple diffraction calculations to obtain sectional…

Image and Video Processing · Electrical Eng. & Systems 2019-03-27 Tomoyoshi Shimobaba , Takayuki Takahashi , Yota Yamamoto , Yutaka Endo , Atsushi Shiraki , Takashi Nishitsuji , Naoto Hoshikawa , Takashi Kakue , Tomoyosh Ito

It is demonstrated that an object distribution can be successfully retrieved from its diffraction pattern or hologram, even if some of the measured intensity samples are missing. The maximum allowable number of missing values depends on the…

Image and Video Processing · Electrical Eng. & Systems 2019-08-28 Tatiana Latychevskaia

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

Imaging through thick scattering media presents significant challenges, particularly for three-dimensional (3D) applications. This manuscript demonstrates a novel scheme for single-image-enabled 3D imaging through such media, treating the…

Optics · Physics 2024-10-10 Long Pan , Yunan Wang , Yijie Lou , Xiaohua Feng

Anisotropic diffusion is a well recognized tool in digital image processing, including edge detection and denoising. We present here a particular nonlinear time-dependent operator together with an appropriate high-order discretization for…

Numerical Analysis · Mathematics 2022-04-29 Lorella Fatone , Daniele Funaro