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The fluorescence microscope is one of the most important tools in modern clinical diagnosis and biological science. However, its expense, size and limited field-of-view (FOV) are becoming bottlenecks in key applications such as large-scale…

Optics · Physics 2012-10-10 Shuo Pang , Chao Han , Mihoko Kato , Paul W. Sternberg , Changhuei Yang

Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…

Optics · Physics 2024-08-29 Jaepil Jo , Herve Hugonnet , Mahn Jae Lee , YongKeun Park

The detection of atmospheric fluorescence light from extensive air showers has become a powerful tool for accurate measurements of the energy and mass of ultra-high energy cosmic ray particles. Employing large area imaging telescopes with…

Instrumentation and Methods for Astrophysics · Physics 2009-07-22 Daniel Kruppke-Hansen , Karl-Heinz Kampert

Controlling the statistical properties of light, namely the fluctuations in photon arrival, entropy and number, is essential for both classical and quantum photonics. While integrated systems provide tunable control over amplitude, phase,…

Automatic detection and tracking of cells in microscopy images are major applications of computer vision technologies in both biomedical research and clinical practice. Though machine learning methods are increasingly common in these…

Computer Vision and Pattern Recognition · Computer Science 2023-12-07 Nikomidisz Eftimiu , Michal Kozubek

Hidden semi-Markov models (HSMMs) are latent variable models which allow latent state persistence and can be viewed as a generalization of the popular hidden Markov models (HMMs). In this paper, we introduce a novel spectral algorithm to…

Machine Learning · Statistics 2016-03-01 Igor Melnyk , Arindam Banerjee

Full-color imaging is significant in digital pathology. Compared with a grayscale image or a pseudo-color image that only contains the contrast information, it can identify and detect the target object better with color texture information.…

Image and Video Processing · Electrical Eng. & Systems 2022-04-26 Yuting Gao , Jiurun Chen , Aiye Wang , An Pan , Caiwen Ma , Baoli Yao

Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Takashi Hato , Pierre C. Dagher , Evan L. Nichols , Cody J. Smith , Kenneth W. Dunn , Scott Howard

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is…

Optics · Physics 2019-04-03 Yoav N. Nygate , Gyanendra Singh , Itay Barnea , Natan T. Shaked

We present an advanced quantum super-resolution imaging technique based on photon statistics measurement and its accurate modeling. Our reconstruction algorithm adapts to any kind of non-Poissonian emitters, outperforming the corresponding…

Fluorescence is a powerful mean to probe information processing in the mammalian brain. However, neuronal tissues are highly heterogeneous and thus opaque to light. A wide set of non-invasive or invasive techniques for scattered light…

Optics · Physics 2020-05-11 Claudio Moretti , Sylvain Gigan

Two-dimensional electronic spectroscopy provides information on coupling and energy transfer between excited states on ultrafast timescales. Only recently, incoherent fluorescence detection has made it possible to combine this method with…

Optics · Physics 2025-05-14 Sanchayeeta Jana , Simon Durst , Lucas Ludwig , Markus Lippitz

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Miniaturized spectrometry system is playing an essential role for materials analysis in the development of in-situ or portable sensing platforms across research and industry. However, there unavoidably exists trade-offs between the…

Optical detection of nanoscale objects without relying on fluorescence is a current challenge due to their extremely weak interaction with light. Resonator-enhanced absorption microscopy is a novel tool to heavily boost the light-matter…

There are successful applications of the holographic AdS/CFT correspondence to high energy and condensed matter physics. We apply the holographic approach to photosynthesis that is an important example of nontrivial quantum phenomena…

High Energy Physics - Theory · Physics 2016-04-12 Irina Aref'eva , Igor Volovich

In numerous industrial processes involving fluids, viscosity is a determinant factor for reaction rates, flows, drying, mixing, etc. Its importance is even more determinant for phenomena observed are at the micro- and nano- scales as in…

Accurate and reliable nuclei identification is an essential part of quantification in microscopy. A range of mathematical and machine learning approaches are used but all methods have limitations. Such limitations include sensitivity to…

Quantitative Methods · Quantitative Biology 2019-04-11 Carl J. Nelson , Philip T. G. Jackson , Boguslaw Obara

Star formation is a multi-scale problem, and only global simulations that account for the connection from the molecular cloud scale gas flow to the accreting protostar can reflect the observed complexity of protostellar systems.…

Astrophysics of Galaxies · Physics 2023-07-28 Rami Al-Belmpeisi , Vito Tuhtan , Mikkel Bregning Christensen , Rajika L Kuruwita , Troels Haugbølle

Optical nanoscopy is crucial in life and materials sciences, revealing subtle cellular processes and nanomaterial properties. Scattering-type Scanning Near-field Optical Microscopy (s-SNOM) provides nanoscale resolution, relying on the…