Large-field-of-view Chip-scale Talbot-grid-based Fluorescence Microscopy
Abstract
The fluorescence microscope is one of the most important tools in modern clinical diagnosis and biological science. However, its expense, size and limited field-of-view (FOV) are becoming bottlenecks in key applications such as large-scale phenotyping and low-resource-setting diagnostics. Here we report a low-cost, compact chip-scale fluorescence-imaging platform, termed the Fluorescence Talbot Microscopy (FTM), which utilizes the Talbot self-imaging effect to enable efficient fluorescence imaging over a large and directly-scalable FOV. The FTM prototype has a resolution of 1.2 microns and an FOV of 3.9 mm x 3.5 mm. We demonstrate the imaging capability of FTM on fluorescently labeled breast cancer cells (SK-BR-3) and HEK cells expressing green fluorescent protein.
Keywords
Cite
@article{arxiv.1210.2424,
title = {Large-field-of-view Chip-scale Talbot-grid-based Fluorescence Microscopy},
author = {Shuo Pang and Chao Han and Mihoko Kato and Paul W. Sternberg and Changhuei Yang},
journal= {arXiv preprint arXiv:1210.2424},
year = {2012}
}