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Imaging and analysis of many single cells hold great potential in our understanding of heterogeneous and complex life systems and in enabling biomedical applications. We here introduce a recently realized image-free "imaging" cytometry…

Image and Video Processing · Electrical Eng. & Systems 2019-03-29 Sadao Ota , Ryoichi Horisaki , Yoko Kawamura , Issei Sato , Hiroyuki Noji

Computational ghost imaging is an imaging technique in which an object is imaged from light collected using a single-pixel detector with no spatial resolution. Recently, ghost cytometry has been proposed for a high-speed cell-classification…

Image and Video Processing · Electrical Eng. & Systems 2019-05-30 Issei Sato

Flow cytometry mainly used for detecting the characteristics of a number of biochemical substances based on the expression of specific markers in cells. It is particularly useful for detecting membrane surface receptors, antigens, ions, or…

Machine Learning · Computer Science 2023-03-17 Yanhua Xu

Flow cytometry is a technique that measures multiple fluorescence and light scatter-associated parameters from individual cells as they flow a single file through an excitation light source. These cells are labeled with antibodies to detect…

Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology,…

Quantitative Methods · Quantitative Biology 2023-05-16 Jacob Beal

Analysis of flow cytometry data is an essential tool for clinical diagnosis of hematological and immunological conditions. Current clinical workflows rely on a manual process called gating to classify cells into their canonical types. This…

Machine Learning · Statistics 2017-11-29 Disi Ji , Eric Nalisnick , Padhraic Smyth

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…

Optics · Physics 2024-08-29 Jaepil Jo , Herve Hugonnet , Mahn Jae Lee , YongKeun Park

Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been…

Applications · Statistics 2010-03-30 Gyemin Lee , William Finn , Clayton Scott

Asymmetric partition of fate determinants during cell division is a hallmark of cell differentiation. Recent work suggested that such a mechanism is hijacked by cancer cells to increase both their phenotypic heterogeneity and plasticity and…

Circulating blood cell clusters (CCCs) containing red blood cells (RBCs), white blood cells(WBCs), and platelets are significant biomarkers linked to conditions like thrombosis, infection, and inflammation. Flow cytometry, paired with…

Computer Vision and Pattern Recognition · Computer Science 2025-10-21 Suqiang Ma , Subhadeep Sengupta , Yao Lee , Beikang Gu , Xianyan Chen , Xianqiao Wang , Yang Liu , Mengjia Xu , Galit H. Frydman , He Li

Ghost imaging is an unconventional imaging technique that generates high resolution images by correlating the intensity of two light beams, neither of which independently contains useful information about the shape of the object. Ghost…

Imaging with hard x-rays is an invaluable tool in medicine, biology, materials science, and cultural heritage. Propagation-based x-ray phase-contrast imaging and tomography have been mostly used to resolve micrometer-scale structures inside…

Image and Video Processing · Electrical Eng. & Systems 2025-06-02 Margie P. Olbinado , David M. Paganin , Yin Cheng , Alexander Rack

Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods…

Assessing the presence of chemical, biological, radiological and nuclear threats is a crucial task which is usually dealt with by analyzing the presence of spectral features in a measured absorption profile. The use of quantum light allows…

Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available. In the area of measurement technology,…

Quantitative Methods · Quantitative Biology 2019-08-20 Yueqin Li , Ata Mahjoubfar , Claire Lifan Chen , Kayvan Reza Niazi , Li Pei , Bahram Jalali

Advancements in cytometry technologies have led to a remarkable increase in the number of markers that can be analyzed simultaneously, presenting significant challenges in data analysis. Traditional approaches, such as dimensional reduction…

Quantitative Methods · Quantitative Biology 2025-07-01 Masahiro Ono

Ghost imaging needs massive measurements to obtain an image with good visibility and the imaging speed is usually very low. In order to realize real-time high-resolution ghost imaging of a target which is located in a scenario with a large…

Image and Video Processing · Electrical Eng. & Systems 2019-05-22 Cheng Zhou , Tian Tian , Chao Gao , Wenli Gong , Lijun Song

Chemical element mapping is an imaging tool that provides essential information on composite materials and it is crucial for a broad range of fields ranging from fundamental science to numerous applications. Methods that exploit x-ray…

Optics · Physics 2021-02-16 Y. Klein , O. Sefi , H. Schwartz , S. Shwartz

Ocean microbes are critical to both ocean ecosystems and the global climate. Flow cytometry, which measures cell optical properties in fluid samples, is routinely used in oceanographic research. Despite decades of accumulated data,…

Methodology · Statistics 2025-04-17 Sangwon Hyun , Tim Coleman , Francois Ribalet , Jacob Bien
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