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Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm…

Software-implementation, via neural networks, of brain-inspired computing approaches underlie many important modern-day computational tasks, from image processing to speech recognition, artificial intelligence and deep learning…

Optics · Physics 2021-02-19 J. Feldmann , N. Youngblood , C. D. Wright , H. Bhaskaran , W. H. P. Pernice

Mammalian whole-brain connectomes are a foundational ingredient for holistic understanding of brains. Indeed, imaging connectomes at sufficient resolution to densely reconstruct cellular morphology and synapses represents a longstanding…

Neurons and Cognition · Quantitative Biology 2025-02-03 Logan Thrasher Collins , Todd Huffman , Randal Koene

Accurate reconstruction of neuronal morphology is essential for classifying cell types and understanding brain connectivity. Recent advances in imaging and reconstruction techniques have greatly expanded the scale and quality of neuronal…

Neurons and Cognition · Quantitative Biology 2025-08-05 Wu Chen , Mingwei Liao , Xueyan Jia , Xiaowei Chen , Chi Xiao , Qingming Luo , Hui Gong , Anan Li

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

Computer Vision and Pattern Recognition · Computer Science 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

Neural circuits can be reconstructed from brain images acquired by serial section electron microscopy. Image analysis has been performed by manual labor for half a century, and efforts at automation date back almost as far. Convolutional…

Computer Vision and Pattern Recognition · Computer Science 2019-05-01 Kisuk Lee , Nicholas Turner , Thomas Macrina , Jingpeng Wu , Ran Lu , H. Sebastian Seung

Reconstruction of neuroanatomy is a fundamental problem in neuroscience. Stochastic expression of colors in individual cells is a promising tool, although its use in the nervous system has been limited due to various sources of variability…

Neurons and Cognition · Quantitative Biology 2017-01-24 Uygar Sümbül , Douglas Roussien , Fei Chen , Nicholas Barry , Edward S. Boyden , Dawen Cai , John P. Cunningham , Liam Paninski

Recent advances in brain clearing and imaging have made it possible to image entire mammalian brains at sub-micron resolution. These images offer the potential to assemble brain-wide atlases of neuron morphology, but manual neuron…

Computer Vision and Pattern Recognition · Computer Science 2022-01-31 Thomas L. Athey , Daniel J. Tward , Ulrich Mueller , Joshua T. Vogelstein , Michael I. Miller

Understanding how networks of neurons process information is one of the key challenges in modern neuroscience. A necessary step to achieve this goal is to be able to observe the dynamics of large populations of neurons over a large area of…

Image and Video Processing · Electrical Eng. & Systems 2022-03-09 Pingfan Song , Herman Verinaz Jadan , Carmel L. Howe , Amanda J. Foust , Pier Luigi Dragotti

Over the last ten years, developments in whole-brain microscopy now allow for high-resolution imaging of intact brains of small rodents such as mice. These complex images contain a wealth of information, but many neuroscience laboratories…

Neurons and Cognition · Quantitative Biology 2021-02-24 Adam L Tyson , Troy W Margrie

Maps of brain microarchitecture are important for understanding neurological function and behavior, including alterations caused by chronic conditions such as neurodegenerative disease. Techniques such as knife-edge scanning microscopy…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Leila Saadatifard , Aryan Mobiny , Pavel Govyadinov , Hien Nguyen , David Mayerich

We present a novel method for quantifying the microscopic structure of brain tissue. It is based on the automated recognition of interpretable features obtained by analyzing the shapes of cells. This contrasts with prevailing methods of…

Computer Vision and Pattern Recognition · Computer Science 2024-04-10 Kui Qian , Litao Qiao , Beth Friedman , Edward O'Donnell , David Kleinfeld , Yoav Freund

NeuroNet is a deep convolutional neural network mimicking multiple popular and state-of-the-art brain segmentation tools including FSL, SPM, and MALPEM. The network is trained on 5,000 T1-weighted brain MRI scans from the UK Biobank Imaging…

Computer Vision and Pattern Recognition · Computer Science 2018-06-13 Martin Rajchl , Nick Pawlowski , Daniel Rueckert , Paul M. Matthews , Ben Glocker

A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently,…

The tracing of neural pathways through large volumes of image data is an incredibly tedious and time-consuming process that significantly encumbers progress in neuroscience. We are exploring deep learning's potential to automate…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Ishtar Nyawira , Kristi Bushman , Iris Qian , Annie Zhang

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

We report the possibility of using a simple neural network for effortless restoration of low-light images inspired by the retina model, which mimics the neurophysiological principles and dynamics of various types of optical neurons. The…

Image and Video Processing · Electrical Eng. & Systems 2022-10-06 Yurui Ming , Yuanyuan Liang

Constructing 3D structures from serial section data is a long standing problem in microscopy. The structure of a fiber reinforced composite material can be reconstructed using a tracking-by-detection model. Tracking-by-detection algorithms…

Computer Vision and Pattern Recognition · Computer Science 2018-05-28 Hongkai Yu , Dazhou Guo , Zhipeng Yan , Wei Liu , Jeff Simmons , Craig P. Przybyla , Song Wang

Unprecedented visual details of biological structures are being revealed by subcellular-resolution whole-brain 3D microscopy data, enabled by recent advances in intact tissue processing and light-sheet fluorescence microscopy (LSFM). These…

Computer Vision and Pattern Recognition · Computer Science 2026-04-01 Minyoung E. Kim , Dae Hee Yun , Aditi V. Patel , Madeline Hon , Webster Guan , Taegeon Lee , Brian Nguyen

Optical imaging of the brain has expanded dramatically in the past two decades. New optics, indicators, and experimental paradigms are now enabling in-vivo imaging from the synaptic to the cortex-wide scales. To match the resulting flood of…

Image and Video Processing · Electrical Eng. & Systems 2024-02-15 Gal Mishne , Adam Charles
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