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Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as calibration measurement and requires no additional hardware components. Our…

Optics · Physics 2025-09-04 Daniël W. S. Cox , Harish Sasikumar , Ivo M. Vellekoop

Quasi-phase-matching (QPM) is a widely adopted technique for mitigating stringent momentum conservation in nonlinear optical processes such as second-harmonic generation (SHG). It effectively compensates for the phase velocity mismatch…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

Breaking the reciprocity of light propagation in photonic integrated circuits (PIC) - especially in the CMOS-compatible silicon-on-insulator platform - is a topic of intense research. However, a practical solution for monolithic integrating…

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

We present a reference-free computational wavefront sensor based on binary amplitude modulation and phase retrieval. The method employs Digital Micro-mirror Device as a programmable amplitude modulator and reconstructs the complex optical…

Optics · Physics 2026-02-12 Ondrej Denk , Jan Pilar , Martin Divoky , Miroslav Cech , Tomas Mocek

Metasurfaces have emerged as a promising platform for integrated nonlinear optics. Nonlocal metasurfaces enable high nonlinear conversion efficiency, while the local ones can offer versatile wavefront control, yet achieving both within a…

Optics · Physics 2026-02-03 Yu Tian , Nuo Wang , Qi Liu , Shuyuan Xiao , Tingting Liu , Olivier J. F. Martin , Ying Gu

Efficient nonlinear frequency conversion in nanophotonics requires not only strong fundamental field but also precise phase matching among distributed nonlinear sources. Here, we develop the two-dimensional Green's function integral method…

Optics · Physics 2025-12-23 Chengkang Liang , Quanying Li , Jiale Xu , Pingqi Gao , Jiancan Yu

Second harmonic generation (SHG) microscopy is a valuable tool for optical microscopy. SHG microscopy is normally performed as a point scanning imaging method, which lacks phase information and is limited in spatial resolution by the…

We present a versatile confocal microscopy setup for optical second harmonic generation (SHG) and multi-photon spectroscopy that enables polarization-resolved studies of semiconductor bulk crystals and low-dimensional structures. The system…

Efficient excitation of a single two-level system usually requires that the driving field is at the same frequency as the atomic transition. However, the scattered laser light in solid-state implementations can dominate over the single…

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

Free-space modulation of light is crucial for many applications, from light detection and ranging to virtual or augmented reality. Traditional means of modulating free-space light involves spatial light modulators based on liquid crystals…

Two dimensional transition metal dichalcogenides exhibit strong excitonic responses, direct bandgaps, and remarkable nonlinear optical properties, making them highly attractive for integrated photonic, optoelectronic, and quantum…