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We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

Wide field-of-view imaging of fast processes in a microscope requires high light intensities motivating the use of lasers as light sources. However, due to their long spatial coherence length lasers are inappropriate for such applications…

Instrumentation and Detectors · Physics 2018-05-23 Tim Stangner , Hanqing Zhang , Tobias Dahlberg , Krister Wiklund , Magnus Andersson

Fourier ptychographic microscopy allows for the collection of images with a high space-bandwidth product at the cost of temporal resolution. In Fourier ptychographic microscopy, the light source of a conventional widefield microscope is…

Image and Video Processing · Electrical Eng. & Systems 2019-01-30 Yi Fei Cheng , Megan Strachan , Zachary Weiss , Moniher Deb , Dawn Carone , Vidya Ganapati

We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven…

Optics · Physics 2019-12-18 Shiming Hu , Wenwen Liu , Junyao Jie , Yizheng Huang , Qingquan Wei , Manqing Tan , Yude Yu

The new generation of laser-based solid-state lighting (SSL) white light sources requires new material systems capable of withstanding, diffusing and converting high intensity laser light. State-of-the-art systems use a blue light emitting…

Inspired by the capability of structured illumination microscopy in subwavelength imaging, many researchers devoted themselves to investigating this methodology. However, due to the free propagating feature of the traditional structured…

Fourier Ptychographic Microscopy (FPM) is a computational technique that achieves a large space-bandwidth product imaging. It addresses the challenge of balancing a large field of view and high resolution by fusing information from multiple…

Optics · Physics 2023-04-21 Ruiqing Sun , Delong Yang , Yao Hu , Qun Hao , Xin Li , Shaohui Zhang

Delivering light to an object is one of the key steps in any imaging exercise. Tools such as LEDs and lasers are available to achieve this. These components are integrated into systems such as microscopy, medical imaging, remote sensing,…

Instrumentation and Detectors · Physics 2022-06-13 Graham M Gibson , Robert Archibald , Mark Main , Akhil Kallepalli

Compact, lightweight and high-performance spatial light modulators (SLMs) are crucial for modern optical technologies. The drive for pixel miniaturization, necessary to improve their performance, has led to a promising alternative, active…

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

Single Pixel (SP) imaging is now a reality in many applications, e.g., biomedical ultrathin endoscope and fluorescent spectroscopy. In this context, many schemes exist to improve the light throughput of these device, e.g., using structured…

Computer Vision and Pattern Recognition · Computer Science 2019-02-19 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

As the quality of optical sensors improves, there is a need for processing large-scale images. In particular, the ability of devices to capture ultra-high definition (UHD) images and video places new demands on the image processing…

Computer Vision and Pattern Recognition · Computer Science 2022-12-23 Tao Wang , Kaihao Zhang , Tianrun Shen , Wenhan Luo , Bjorn Stenger , Tong Lu

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

We demonstrate a simple and cost-efficient scanning confocal microscope setup for use in advanced instructional physics laboratories. The setup is constructed from readily available commercial products, and the implementation of a…

Physics Education · Physics 2023-05-03 A. Reguilon , W. Bethard , E. Brekke

This paper introduces a single-pixel HyperSpectral (HS) imaging framework based on Fourier Transform Interferometry (FTI). By combining a space-time coding of the light illumination with partial interferometric observations of a collimated…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Amirafshar Moshtaghpour , José M. Bioucas-Dias , Laurent Jacques

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging…

Reconstituted cytoskeleton composites have emerged as a valuable model system for studying non equilibrium soft matter. Faithful capture of the dynamics of these three dimensional, dense networks calls for optical sectioning often…

Instrumentation and Detectors · Physics 2023-05-01 Nathan Felcher , Daisy Achiriloaie , Brian Lee , Ryan McGorty , Janet Sheung

The CMS experiment at the CERN LHC will be upgraded to accommodate the 5-fold increase in the instantaneous luminosity expected at the High-Luminosity LHC (HL-LHC). Concomitant with this increase will be an increase in the number of…

Instrumentation and Detectors · Physics 2021-04-28 B. Acar , G. Adamov , C. Adloff , S. Afanasiev , N. Akchurin , B. Akgün , M. Alhusseini , J. Alison , G. Altopp , M. Alyari , S. An , S. Anagul , I. Andreev , M. Andrews , P. Aspell , I. A. Atakisi , O. Bach , A. Baden , G. Bakas , A. Bakshi , P. Bargassa , D. Barney , E. Becheva , P. Behera , A. Belloni , T. Bergauer , M. Besancon , S. Bhattacharya , S. Bhattacharya , D. Bhowmik , P. Bloch , A. Bodek , M. Bonanomi , A. Bonnemaison , S. Bonomally , J. Borg , F. Bouyjou , D. Braga , J. Brashear , E. Brondolin , P. Bryant , J. Bueghly , B. Bilki , B. Burkle , A. Butler-Nalin , S. Callier , D. Calvet , X. Cao , B. Caraway , S. Caregari , L. Ceard , Y. C. Cekmecelioglu , G. Cerminara , N. Charitonidis , R. Chatterjee , Y. M. Chen , Z. Chen , K. y. Cheng , S. Chernichenko , H. Cheung , C. H. Chien , S. Choudhury , D. Čoko , G. Collura , F. Couderc , I. Dumanoglu , D. Dannheim , P. Dauncey , A. David , G. Davies , E. Day , P. DeBarbaro , F. De Guio , C. de La Taille , M. De Silva , P. Debbins , E. Delagnes , J. M. Deltoro , G. Derylo , P. G. Dias de Almeida , D. Diaz , P. Dinaucourt , J. Dittmann , M. Dragicevic , S. Dugad , V. Dutta , S. Dutta , J. Eckdahl , T. K. Edberg , M. El Berni , S. C. Eno , Yu. Ershov , P. Everaerts , S. Extier , F. Fahim , C. Fallon , B. A. Fontana Santos Alves , E. Frahm , G. Franzoni , J. Freeman , T. French , E. Gurpinar Guler , Y. Guler , M. Gagnan , P. Gandhi , S. Ganjour , A. Garcia-Bellido , Z. Gecse , Y. Geerebaert , H. Gerwig , O. Gevin , W. Gilbert , A. Gilbert , K. Gill , C. Gingu , S. Gninenko , A. Golunov , I. Golutvin , T. Gonzalez , N. Gorbounov , L. Gouskos , Y. Gu , F. Guilloux , E. Gülmez , M. Hammer , A. Harilal , K. Hatakeyama , A. Heering , V. Hegde , U. Heintz , V. Hinger , N. Hinton , J. Hirschauer , J. Hoff , W. S. Hou , C. Isik , J. Incandela , S. Jain , H. R. Jheng , U. Joshi , O. Kara , V. Kachanov , A. Kalinin , R. Kameshwar , A. Kaminskiy , A. Karneyeu , O. Kaya , M. Kaya , A. Khukhunaishvili , S. Kim , K. Koetz , T. Kolberg , A. Kristić , M. Krohn , K. Krüger , N. Kulagin , S. Kulis , S. Kunori , C. M. Kuo , V. Kuryatkov , S. Kyre , O. K. Köseyan , Y. Lai , K. Lamichhane , G. Landsberg , J. Langford , M. Y. Lee , A. Levin , A. Li , B. Li , J. -H. Li , H. Liao , D. Lincoln , L. Linssen , R. Lipton , Y. Liu , A. Lobanov , R. S. Lu , I. Lysova , A. M. Magnan , F. Magniette , A. A. Maier , A. Malakhov , I. Mandjavize , M. Mannelli , J. Mans , A. Marchioro , A. Martelli , P. Masterson , B. Meng , T. Mengke , A. Mestvirishvili , I. Mirza , S. Moccia , I. Morrissey , T. Mudholkar , J. Musić , I. Musienko , S. Nabili , A. Nagar , A. Nikitenko , D. Noonan , M. Noy , K. Nurdan , C. Ochando , B. Odegard , N. Odell , Y. Onel , W. Ortez , J. Ozegović , L. Pacheco Rodriguez , E. Paganis , D. Pagenkopf , V. Palladino , S. Pandey , F. Pantaleo , C. Papageorgakis , I. Papakrivopoulos , J. Parshook , N. Pastika , M. Paulini , P. Paulitsch , T. Peltola , R. Pereira Gomes , H. Perkins , P. Petiot , F. Pitters , F. Pitters , H. Prosper , M. Prvan , I. Puljak , T. Quast , R. Quinn , M. Quinnan , K. Rapacz , L. Raux , G. Reichenbach , M. Reinecke , M. Revering , A. Rodriguez , T. Romanteau , A. Rose , M. Rovere , A. Roy , P. Rubinov , R. Rusack , A. E. Simsek , U. Sozbilir , O. M. Sahin , A. Sanchez , R. Saradhy , T. Sarkar , M. A. Sarkisla , J. B. Sauvan , I. Schmidt , M. Schmitt , E. Scott , C. Seez , F. Sefkow , S. Sharma , I. Shein , A. Shenai , R. Shukla , E. Sicking , P. Sieberer , Y. Sirois , V. Smirnov , E. Spencer , A. Steen , J. Strait , T. Strebler , N. Strobbe , J. W. Su , E. Sukhov , L. Sun , M. Sun , C. Syal , B. Tali , U. G. Tok , A. Kayis Topaksu , C. L. Tan , I. Tastan , T. Tatli , R. Thaus , S. Tekten , D. Thienpont , T. Pierre-Emile , E. Tiras , M. Titov , D. Tlisov , J. Troska , Z. Tsamalaidze , G. Tsipolitis , A. Tsirou , N. Tyurin , S. Undleeb , D. Urbanski , V. Ustinov , A. Uzunian , M. van de Klundert , J. Varela , M. Velasco , M. Vicente Barreto Pinto , P. M. da Silva , T. Virdee , R. Vizinho de Oliveira , J. Voelker , E. Voirin , Z. Wang , X. Wang , F. Wang , M. Wayne , S. N. Webb , M. Weinberg , A. Whitbeck , D. White , R. Wickwire , J. S. Wilson , H. Y. Wu , L. Wu , C. H Yeh , R. Yohay , G. B. Yu , S. S. Yu , D. Yu , F. Yumiceva , A. Zacharopoulou , N. Zamiatin , A. Zarubin , S. Zenz , H. Zhang , J. Zhang
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