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Extracting depth information from photon-limited, defocused images is challenging because depth from defocus (DfD) relies on accurate estimation of defocus blur, which is fundamentally sensitive to image noise. We present a novel approach…

Computer Vision and Pattern Recognition · Computer Science 2025-12-09 Wei Xu , Charles James Wagner , Junjie Luo , Qi Guo

Photoacoustic tomography leverages ultrasound's deep tissue penetration to retrieve optical absorption contrast well beyond the optical diffusion limit. Conventional photoacoustic systems rely on externally delivered light and are therefore…

Optics · Physics 2026-01-06 David C. Garrett , Lihong V. Wang

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

We propose and demonstrate a new phase retrieval method for imaging through random media. Although methods to recover the Fourier amplitude through random distortions are well established, recovery of the Fourier phase has been a more…

Optics · Physics 2021-05-14 Byungjae Hwang , Taeseong Woo , Cheolwoo Ahn , Jung-Hoon Park

Noninvasive optical imaging through dynamic scattering media has numerous important biomedical applications but still remains a challenging task. While standard diffuse imaging methods measure optical absorption or fluorescent emission, it…

Recently, fluorescence-based optical techniques have emerged as a powerful tool to probe information in the mammalian brain. However, tissue heterogeneities prevent clear imaging of deep neuron bodies due to light scattering. While several…

This paper presents the results of a Fresnel Interferometric Array testbed. This new concept of imager involves diffraction focussing by a thin foil, in which many thousands of punched subapertures form a pattern related to a Fresnel zone…

Astrophysics · Physics 2008-08-07 Denis Serre , Laurent Koechlin , Paul Deba

We present simulations of an efficient high numerical aperture solid immersion metalens concept for fluorescence microscopy. The technique exploits the preferential emission of interfacial dipoles into a high refractive index substrate…

Optics · Physics 2022-01-12 Zhiheng Li , Zequan Chen , Rupert F. Oulton , Ming Fu

Time-of-Flight (ToF) sensors efficiently capture scene depth, but the nonlinear depth construction procedure often results in extremely large noise variance or even invalid areas. Recent methods based on deep neural networks (DNNs) achieve…

Computer Vision and Pattern Recognition · Computer Science 2025-03-26 Changyong He , Jin Zeng , Jiawei Zhang , Jiajie Guo

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at sub-micron…

Optics · Physics 2021-04-08 Yi Zhang , Haonan Zong , Cheng Zong , Yuying Tan , Meng Zhang , Yuewei Zhan , Ji-Xin Cheng

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

In this paper we apply digital in-line holography to image opaque objects through a thick plano-concave pipe. Opaque fibers and opaque particles are considered}. Analytical expression of the intensity distribution in the CCD sensor plane is…

Optics · Physics 2012-12-21 Nicolas Verrier , Coëtmellec Sébastien , Brunel Marc , Lebrun Denis

We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…

Optics · Physics 2024-10-22 Richard G. Baird , Apratim Majumder , Rajesh Menon

Interferometry provides one of the possible routes to ultra-high angular resolution for X-ray and gamma-ray astronomy. Sub-micro-arc-second angular resolution, necessary to achieve objectives such as imaging the regions around the event…

Instrumentation and Methods for Astrophysics · Physics 2009-07-31 G. K. Skinner , J. F. Krizmanic

Human ability to visualize an image is usually hindered by optical scattering. Recent extensive studies have promoted imaging technique through turbid materials to a reality where color image can be restored behind scattering media in real…

With the advent of mobile phone photography and point-and-shoot cameras, deep-burst imaging is widely used for a number of photographic effects such as depth of field, super-resolution, motion deblurring, and image denoising. In this work,…

Computer Vision and Pattern Recognition · Computer Science 2023-06-19 Arijit Mallick , Raphael Braun , Hendrik PA Lensch

In this work we present a dedicated experimental facility and an image-based method for measuring water depth in a radially spreading dam-break wave propagating over a horizontal plane. The facility consists of a prismatic reservoir…

Fluid Dynamics · Physics 2026-01-27 Elia Buono , Roberto Bosio , Andrea Cagninei , Davide Poggi

Capturing images under extremely low-light conditions poses significant challenges for the standard camera pipeline. Images become too dark and too noisy, which makes traditional enhancement techniques almost impossible to apply. Recently,…

Computer Vision and Pattern Recognition · Computer Science 2021-11-23 Ahmet Serdar Karadeniz , Erkut Erdem , Aykut Erdem

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka