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We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

The quantitative analysis of cellular membranes helps understanding developmental processes at the cellular level. Particularly 3D microscopic image data offers valuable insights into cell dynamics, but error-free automatic segmentation…

Computer Vision and Pattern Recognition · Computer Science 2018-10-17 Dennis Eschweiler , Thiago V. Spina , Rohan C. Choudhury , Elliot Meyerowitz , Alexandre Cunha , Johannes Stegmaier

This paper presents a method for time-lapse 3D cell analysis. Specifically, we consider the problem of accurately localizing and quantitatively analyzing sub-cellular features, and for tracking individual cells from time-lapse 3D confocal…

Computer Vision and Pattern Recognition · Computer Science 2022-08-18 Jiaxiang Jiang , Amil Khan , S. Shailja , Samuel A. Belteton , Michael Goebel , Daniel B. Szymanski , B. S. Manjunath

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

Computer Vision and Pattern Recognition · Computer Science 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

Image and Video Processing · Electrical Eng. & Systems 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

Modeling the 3D structures of cells and tissues is crucial in biology. Sequential cross-sectional images from electron microscopy provide high-resolution intracellular structure information. The segmentation of complex cell structures…

Quantitative Methods · Quantitative Biology 2025-02-14 Jin Kousaka , Atsuko H. Iwane , Yuichi Togashi

Automated surface segmentation is important and challenging in many medical image analysis applications. Recent deep learning based methods have been developed for various object segmentation tasks. Most of them are a classification based…

Computer Vision and Pattern Recognition · Computer Science 2019-09-25 Leixin Zhou , Zisha Zhong , Abhay Shah , Bensheng Qiu , John Buatti , Xiaodong Wu

3D cell segmentation methods are often hindered by \emph{oversegmentation}, where a single cell is incorrectly split into multiple fragments. This degrades the final segmentation quality and is notoriously difficult to resolve, as…

Computer Vision and Pattern Recognition · Computer Science 2025-12-22 Peter Chen , Bryan Chang , Olivia A Creasey , Julie Beth Sneddon , Zev J Gartner , Yining Liu

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

The progress in imaging techniques have allowed the study of various aspect of cellular mechanisms. To isolate individual cells in live imaging data, we introduce an elegant image segmentation framework that effectively extracts cell…

Computer Vision and Pattern Recognition · Computer Science 2016-02-18 Arnaud Browet , Christophe De Vleeschouwer , Laurent Jacques , Navrita Mathiah , Bechara Saykali , Isabelle Migeotte

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Identify the cells' nuclei is the important point for most medical analyses. To assist doctors finding the accurate cell' nuclei location automatically is highly demanded in the clinical practice. Recently, fully convolutional neural…

Computer Vision and Pattern Recognition · Computer Science 2019-11-25 Tianyang Zhang , Rui Ma

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

Imaging assays of cellular function, especially those using fluorescent stains, are ubiquitous in the biological and medical sciences. Despite advances in computer vision, such images are often analyzed using only manual or rudimentary…

Computer Vision and Pattern Recognition · Computer Science 2017-06-06 Lena R. Bartell , Lawrence J. Bonassar , Itai Cohen

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

The advancement of the neuroscientific imaging techniques has produced an unprecedented size of neural cell imaging data, which calls for automated processing. In particular, identification of cells from two photon images demands…

Image and Video Processing · Electrical Eng. & Systems 2019-09-26 Si-Baek Seong , Hae-Jeong Park

The presented algorithms for segmentation and tracking follow a 3-step approach where we detect, track and finally segment nuclei. In the preprocessing phase, we detect centroids of the cell nuclei using a convolutional neural network (CNN)…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Dennis Eschweiler , Johannes Stegmaier

Image segmentation is considered to be one of the critical tasks in hyperspectral remote sensing image processing. Recently, convolutional neural network (CNN) has established itself as a powerful model in segmentation and classification by…

Computer Vision and Pattern Recognition · Computer Science 2017-12-29 Fahim Irfan Alam , Jun Zhou , Alan Wee-Chung Liew , Xiuping Jia , Jocelyn Chanussot , Yongsheng Gao
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