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Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

Computer Vision and Pattern Recognition · Computer Science 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

We present a dual-stage neural network architecture for analyzing fine shape details from microscopy recordings in 3D. The system, tested on red blood cells, uses training data from both healthy donors and patients with a congenital blood…

Quantitative Methods · Quantitative Biology 2020-05-29 G. Simionato , K. Hinkelmann , R. Chachanidze , P. Bianchi , E. Fermo , R. van Wijk , M. Leonetti , C. Wagner , L. Kaestner , S. Quint

The locations of different mRNA molecules can be revealed by multiplexed in situ RNA detection. By assigning detected mRNA molecules to individual cells, it is possible to identify many different cell types in parallel. This in turn enables…

Information Theory · Computer Science 2023-12-08 Axel Andersson , Andrea Behanova , Carolina Wählby , Filip Malmberg

In this work, we describe a method for large-scale 3D cell-tracking through a segmentation selection approach. The proposed method is effective at tracking cells across large microscopy datasets on two fronts: (i) It can solve problems…

Computer Vision and Pattern Recognition · Computer Science 2024-04-15 Jordão Bragantini , Merlin Lange , Loïc Royer

Cell counting is a ubiquitous, yet tedious task that would greatly benefit from automation. From basic biological questions to clinical trials, cell counts provide key quantitative feedback that drive research. Unfortunately, cell counting…

Computer Vision and Pattern Recognition · Computer Science 2018-03-02 Carlos X. Hernández , Mohammad M. Sultan , Vijay S. Pande

Advances in fluorescence microscopy enable acquisition of 3D image volumes with better image quality and deeper penetration into tissue. Segmentation is a required step to characterize and analyze biological structures in the images and…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Chichen Fu , Soonam Lee , David Joon Ho , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be made in tools that rely on classical methods for segmentation,…

Quantitative Methods · Quantitative Biology 2024-03-15 Nodar Gogoberidze , Beth A. Cimini

We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

Accurately counting cells in microscopic images is important for medical diagnoses and biological studies, but manual cell counting is very tedious, time-consuming, and prone to subjective errors, and automatic counting can be less accurate…

Computer Vision and Pattern Recognition · Computer Science 2019-03-25 Shenghua He , Kyaw Thu Minn , Lilianna Solnica-Krezel , Mark Anastasio , Hua Li

Increasing data set sizes of 3D microscopy imaging experiments demand for an automation of segmentation processes to be able to extract meaningful biomedical information. Due to the shortage of annotated 3D image data that can be used for…

Image and Video Processing · Electrical Eng. & Systems 2022-02-02 Dennis Eschweiler , Richard S. Smith , Johannes Stegmaier

Automated segmentation approaches are crucial to quantitatively analyze large-scale 3D microscopy images. Particularly in deep tissue regions, automatic methods still fail to provide error-free segmentations. To improve the segmentation…

Computer Vision and Pattern Recognition · Computer Science 2017-10-19 Johannes Stegmaier , Thiago V. Spina , Alexandre X. Falcão , Andreas Bartschat , Ralf Mikut , Elliot Meyerowitz , Alexandre Cunha

Semantic segmentation of microscopy cell images by deep learning is a significant technique. We considered that the Transformers, which have recently outperformed CNNs in image recognition, could also be improved and developed for cell…

Computer Vision and Pattern Recognition · Computer Science 2026-04-29 Hinako Mitsuoka , Kazuhiro Hotta

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Automated cell segmentation is crucial for various biological and medical applications, facilitating tasks like cell counting, morphology analysis, and drug discovery. However, manual segmentation is time-consuming and prone to…

Machine Learning · Computer Science 2024-09-13 Aaron Rock Menezes , Bharath Ramsundar

Automatic cell segmentation is an essential step in the pipeline of computer-aided diagnosis (CAD), such as the detection and grading of breast cancer. Accurate segmentation of cells can not only assist the pathologists to make a more…

Image and Video Processing · Electrical Eng. & Systems 2020-12-29 Muyi Sun , Zeyi Yao , Guanhong Zhang

Reliable cell segmentation and classification from biomedical images is a crucial step for both scientific research and clinical practice. A major challenge for more robust segmentation and classification methods is the large variations in…

Cell Behavior · Quantitative Biology 2017-10-31 Mo Zhang , Xiang Li , Mengjia Xu , Quanzheng Li

Cell boundary information is crucial for analyzing cell behaviors from time-lapse microscopy videos. Existing supervised cell segmentation tools, such as ImageJ, require tuning various parameters and rely on restrictive assumptions about…

Applications · Statistics 2026-01-27 Laura Baracaldo , Blythe King , Haoran Yan , Yizi Lin , Nina Miolane , Mengyang Gu