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Recent research in computational imaging largely focuses on developing machine learning (ML) techniques for image reconstruction, which requires large-scale training datasets consisting of measurement data and ground-truth images. However,…

Image and Video Processing · Electrical Eng. & Systems 2023-09-26 Maximilian B. Kiss , Sophia B. Coban , K. Joost Batenburg , Tristan van Leeuwen , Felix Lucka

Photon-Counting Computed Tomography (PCCT) is a novel imaging modality that simultaneously acquires volumetric data at multiple X-ray energy levels, generating separate volumes that capture energy-dependent attenuation properties.…

Human-Computer Interaction · Computer Science 2025-08-21 Mohit Sharma , Emma Nilsson , Martin Falk , Talha Bin Masood , Lee Jollans , Anders Persson , Tino Ebbers , Ingrid Hotz

Atomic structures and adatom geometries of surfaces encode information about the thermodynamics and kinetics of the processes that lead to their formation, and which can be captured by a generative physical model. Here we develop a workflow…

Data management is a critical component of modern experimental workflows. As data generation rates increase, transferring data from acquisition servers to processing servers via conventional file-based methods is becoming increasingly…

Instrumentation and Detectors · Physics 2024-07-04 Samuel S. Welborn , Chris Harris , Stephanie M. Ribet , Georgios Varnavides , Colin Ophus , Bjoern Enders , Peter Ercius

Efforts to utilize growing volumes of clinical imaging data to generate tumor evaluations continue to require significant manual data wrangling owing to the data heterogeneity. Here, we propose an artificial intelligence-based solution for…

Since decades, many techniques have been developed for curing cancer. Here, an effort has been made to cure tumor by inserting a cryoprobe into the target region which is connected to a cryogenic mechanism. This technique is presently used…

Medical Physics · Physics 2022-12-23 G Sai Krishna , Anup Paul

A major challenge in single particle reconstruction methods using cryo-electron microscopy is to attain a resolution sufficient to interpret fine details in three-dimensional (3D) macromolecular structures. Obtaining high resolution 3D…

Numerical Analysis · Mathematics 2013-07-23 Lanhui Wang , Yoel Shkolnisky , Amit Singer

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the…

Computational Physics · Physics 2018-03-20 Amit Singer

The central problem in cryo-electron microscopy (cryo-EM) is to recover the 3D structure from noisy 2D projection images which requires estimating the missing projection angles (poses). Recent methods attempted to solve the 3D…

Computer Vision and Pattern Recognition · Computer Science 2023-02-15 Lin Yao , Ruihan Xu , Zhifeng Gao , Guolin Ke , Yuhang Wang

Large-scale electrification is vital to addressing the climate crisis, but several scientific and technological challenges remain to fully electrify both the chemical industry and transportation. In both of these areas, new electrochemical…

There has been an increasing interest in atom probe tomography (APT) to characterise hydrated and biological materials. A major benefit of APT compared to microscopy techniques more commonly used in biology is its combination of outstanding…

Applied Physics · Physics 2023-11-13 Florant Exertier , Levi Tegg , Adam Taylor , Julie M. Cairney , Jing Fu , Ross K. W. Marceau

Cryo-electron microscopy (cryo-EM) studies using single particle reconstruction is extensively used to reveal structural information of macromolecular complexes. Aiming at the highest achievable resolution, state of the art electron…

Computer Vision and Pattern Recognition · Computer Science 2020-05-04 Ramin Norousi , Stephan Wickles , Thomas Becker , Roland Beckmann , Volker J. Schmid , Achim Tresch

We present an end-to-end automated workflow that uses large-scale remote compute resources and an embedded GPU platform at the edge to enable AI/ML-accelerated real-time analysis of data collected for x-ray ptychography. Ptychography is a…

Computer Vision and Pattern Recognition · Computer Science 2023-04-11 Anakha V Babu , Tekin Bicer , Saugat Kandel , Tao Zhou , Daniel J. Ching , Steven Henke , Siniša Veseli , Ryan Chard , Antonino Miceli , Mathew Joseph Cherukara

We present a unified formulation and model for three motion and 3D perception tasks: optical flow, rectified stereo matching and unrectified stereo depth estimation from posed images. Unlike previous specialized architectures for each…

Computer Vision and Pattern Recognition · Computer Science 2023-07-27 Haofei Xu , Jing Zhang , Jianfei Cai , Hamid Rezatofighi , Fisher Yu , Dacheng Tao , Andreas Geiger

Cryo-electron microscopy (cryo-EM) is a widely used technique for recovering the 3-D structure of biological molecules from a large number of experimentally generated noisy 2-D tomographic projection images of the 3-D structure, taken from…

Numerical Analysis · Mathematics 2025-11-17 Jeremy Hoskins , Yuehaw Khoo , Oscar Mickelin , Amit Singer , Yuguan Wang

Classifying structural variability in noisy projections of biological macromolecules is a central problem in Cryo-EM. In this work, we build on a previous method for estimating the covariance matrix of the three-dimensional structure…

Computer Vision and Pattern Recognition · Computer Science 2015-12-08 Joakim Andén , Eugene Katsevich , Amit Singer

Echocardiography is essential to modern cardiology. However, human interpretation limits high throughput analysis, limiting echocardiography from reaching its full clinical and research potential for precision medicine. Deep learning is a…

Computer Vision and Pattern Recognition · Computer Science 2017-06-28 Ali Madani , Ramy Arnaout , Mohammad Mofrad , Rima Arnaout

In a comprehensive study on several samples we demonstrate for our laboratory-based computed tomography system resolutions down to 150nm. The achieved resolution is validated by imaging com-mon test structures in 2D and Fourier Shell…

Instrumentation and Detectors · Physics 2021-02-15 Dominik Müller , Jonas Graetz , Andreas Balles , Simon Stier , Randolf Hanke , Christian Fella

Electron tomography in materials science has flourished with the demand to characterize nanoscale materials in three dimensions (3D). Access to experimental data is vital for developing and validating reconstruction methods that improve…

Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of protein function. CryoEM provides direct visualization of individual macromolecules sampling different conformational and compositional states.…

Biomolecules · Quantitative Biology 2021-08-04 Muyuan Chen , Steven Ludtke