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An efficient method is reported for rapid identification of bacterial or mycobacterial infection in a suspected clinical/biological sample. The method is based on the fact that the ring methylene protons of cyclic fatty acids (constituting…

Biological Physics · Physics 2014-12-30 Anupam Guleria , Nitin K Bajpai , Atul Rawat , C L Khetrapal , Narayan Prasad , Dinesh Kumar

Skin and soft tissue infections (SSTIs) are among the most frequently observed diseases in ambulatory and hospital settings. Resistance of diverse bacterial pathogens to antibiotics is a significant cause of severe SSTIs, and treatment…

Machine Learning · Computer Science 2022-03-01 Farnaz H. Foomani , Shahzad Mirza , Sahjid Mukhida , Kannuri Sriram , Zeyun Yu , Aayush Gupta , Sandeep Gopalakrishnan

Dynabeads are superparamagnetic particles used for immunomagnetic purification of cells and biomolecules. Post-capture, however, target identification relies on tedious culturing, fluorescence staining and/or target amplification. Raman…

Biological Physics · Physics 2023-07-25 Jongwan Lee , Marissa McDonald , Nikiwe Mhlanga , Jeon Woong Kang , Rohit Karnik , Loza F. Tadesse

Raman spectroscopy in combination with machine learning has significant promise for applications in clinical settings as a rapid, sensitive, and label-free identification method. These approaches perform well in classifying data that…

Machine Learning · Computer Science 2021-11-12 Yaroslav Balytskyi , Justin Bendesky , Tristan Paul , Guy Hagen , Kelly McNear

Deep learning has dramatically improved the performance in many application areas such as image classification, object detection, speech recognition, drug discovery and etc since 2012. Where deep learning algorithms promise to discover the…

Image and Video Processing · Electrical Eng. & Systems 2020-03-25 X. F. Xu , S. Talbot , T. Selvaraja

Raman spectroscopy is a powerful analytical tool with applications ranging from quality control to cutting edge biomedical research. One particular area which has seen tremendous advances in the past decade is the development of powerful…

Signal Processing · Electrical Eng. & Systems 2020-06-19 M. Hamed Mozaffari , Li-Lin Tay

We report here on the ability of elastic light scattering in discriminating Gram+, Gram-and yeasts at an early stage of growth (6h). Our technique is non-invasive, low cost and does require neither skilled operators nor reagents. Therefore…

Accurate detection and analysis of traces of persistent organic pollutants in water is important in many areas, including environmental monitoring and food quality control, due to their long environmental stability and potential…

Machine Learning · Computer Science 2024-02-02 Vishnu Jayaprakash , Jae Bem You , Chiranjeevi Kanike , Jinfeng Liu , Christopher McCallum , Xuehua Zhang

Machine learning methods have found many applications in Raman spectroscopy, especially for the identification of chemical species. However, almost all of these methods require non-trivial preprocessing such as baseline correction and/or…

Machine Learning · Computer Science 2018-02-07 Jinchao Liu , Margarita Osadchy , Lorna Ashton , Michael Foster , Christopher J. Solomon , Stuart J. Gibson

Background: Microbiota have evolved to acclimate themselves to many environments. Humanity is become ever increasingly medicated and many of those medications are antibiotics. Sadly, Microbiota are adapting to medication and with each…

Genomics · Quantitative Biology 2012-09-27 Casey Richardson

The diagnosis of prostate cancer is challenging due to the heterogeneity of its presentations, leading to the over diagnosis and treatment of non-clinically important disease. Accurate diagnosis can directly benefit a patient's quality of…

Raman spectroscopy is frequently used to identify composition, structure and layer thickness of 2D materials. Here, we describe an efficient first-principles workflow for calculating resonant first-order Raman spectra of solids within…

Materials Science · Physics 2020-07-15 A. Taghizadeh , U. Leffers , T. G. Pedersen , K. S. Thygesen

Raman spectroscopy, a photonic modality based on the inelastic backscattering of coherent light, is a valuable asset to the intraoperative sensing space, offering non-ionizing potential and highly-specific molecular fingerprint-like…

Image and Video Processing · Electrical Eng. & Systems 2024-01-18 Ashutosh Raman , Ren A. Odion , Kent K. Yamamoto , Weston Ross , Tuan Vo-Dinh , Patrick J. Codd

Raman spectroscopy provides spectral information related to the specific molecular structures of substances and has been well established as a powerful tool for studying biological tissues and diagnosing diseases. This article reviews…

Raman microscopy is a powerful method combining non-invasiveness with no special sample preparation. Because of this remarkable simplicity, it has been widely exploited in many fields, ranging from life and materials sciences, to…

In chemical processing and bioprocessing, conventional online sensors are limited to measure only basic process variables like pressure and temperature, pH, dissolved O and CO$_2$ and viable cell density (VCD). The concentration of other…

Quantitative Methods · Quantitative Biology 2020-05-07 Semion Rozov

The rapid and accurate detection of biochemical compositions in fish is a crucial real-world task that facilitates optimal utilization and extraction of high-value products in the seafood industry. Raman spectroscopy provides a promising…

Diagnosis of fungal infections can rely on microscopic examination, however, in many cases, it does not allow unambiguous identification of the species due to their visual similarity. Therefore, it is usually necessary to use additional…

Computer Vision and Pattern Recognition · Computer Science 2020-10-14 Bartosz Zieliński , Agnieszka Sroka-Oleksiak , Dawid Rymarczyk , Adam Piekarczyk , Monika Brzychczy-Włoch

A bacterial colony may develop a small number of cells genetically identical to, but phenotypically different from other normally growing bacteria. These so-called persister cells keep themselves in a dormant state and thus are insensitive…

Molecular Networks · Quantitative Biology 2010-02-11 Yan Fu , Meng Zhu , Jianhua Xing

Coherent Raman scattering provides highly sensitive vibrational analysis through nonlinear light-matter interactions. However, its application to metal interfaces has remained challenging because the intrinsically large non-resonant…