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Confocal time resolved single-molecule spectroscopy using pulsed laser excitation and synchronized multi channel time correlated single photon counting (TCSPC) provides detailed information about the conformational changes of a biological…

Biological Physics · Physics 2009-11-13 N. Zarrabi , M. G. Dueser , S. Ernst , R. Reuter , G. D. Glick , S. D. Dunn , J. Wrachtrup , M. Boersch

Adhesion-dependent cells actively sense the mechanical properties of their environment through mechanotransductory processes at focal adhesions, which are integrin-based contacts connecting the extracellular matrix to the cytoskeleton. Here…

Subcellular Processes · Quantitative Biology 2007-05-23 Ulrich S. Schwarz , Thorsten Erdmann , Ilka B. Bischofs

Photosensitive proteins embedded in the cell membrane (about 5 nm thickness) act as photoactivated proton pumps, ion gates, enzymes, or more generally, as initiators of stimuli for the cell activity. They are composed of a protein backbone…

The enzyme FoF1-ATP synthase provides the 'chemical energy currency' adenosine triphosphate (ATP) for living cells. Catalysis is driven by mechanochemical coupling of subunit rotation within the enzyme with conformational changes in the…

Biomolecules · Quantitative Biology 2015-06-04 Stefan Ernst , Monika G. Dueser , Nawid Zarrabi , Michael Boersch

Recent experimental findings reveal non-conventional fluorescence emission in biological systems devoid of conjugated bonds or aromatic compounds, termed \textit{Non-Aromatic Fluorescence} (NAF). This phenomenon is exclusive to aggregated…

Mechanical coupling between a cell and substrate relies on focal adhesions, clusters of adhesion proteins linking stress fibers (bundles of actin proteins) inside the cell with surrounding tissue. Focal adhesions have been demonstrated to…

Soft Condensed Matter · Physics 2007-05-23 Thomas Bickel , Robijn Bruinsma

Electrostatic gating offers elegant ways to simultaneously strain and dope atomically thin membranes. Here, we report on a detailed \textit{in situ} Raman scattering study on graphene, suspended over a Si/SiO$_2$ substrate. In such a…

Mesoscale and Nanoscale Physics · Physics 2017-01-30 Dominik Metten , Guillaume Froehlicher , Stéphane Berciaud

The Penning-trap electronic-detection technique that offers the precision and sensitivity requested in mass spectrometry for fundamental studies in nuclear and particle physics has not been proven yet to be universal. This has motivated the…

A compact versatile photoacoustic (PA) sensor for trace gas detection is reported. The sensor is based on an integrating sphere as the PA absorption cell with an organ pipe tube attached to increase the sensitivity of the PA sensor. The…

Smart wearables sense and process information from the user's body and environment and report results of their analysis as electrical signals. Conventional electronic sensors and controllers are commonly, sometimes augmented by recent…

Emerging Technologies · Computer Science 2020-09-15 Andrew Adamatzky , Anna Nikolaidou , Antoni Gandia , Alessandro Chiolerio , Mohammad Mahdi Dehshib

Accurate detection and characterization of nanoparticles within confined spaces is crucial for applications ranging from nanofluidics to biotechnology. We present a novel approach that combines interferometric scattering (iSCAT) detection…

Through the development of new non-fullerene electron acceptor (NFA) materials, such as Y6 and its molecular derivatives, the power conversion efficiencies of organic photovoltaics (OPVs) have now exceeded 19%. However, despite this rapid…

Subwavelength imaging by microsphere lenses is a promising label-free super-resolution imaging technique. There is a growing interest to use live cells to replace the widely used non-biological microsphere lenses. In this work, we…

Optics · Physics 2021-07-06 Chunlei Jiang , Hangyu Yue , Bing Yan , Taiji Dong , Xiangyu Cui , Zengbo Wang

The hexagonal multiferroic oxide YMnO$_3$ has demonstrated applications in various fields and is widely researched due to its interesting properties. Since Mn(3d)--O(2p) interactions predominate close to the Fermi level, doping in the…

Materials Science · Physics 2025-10-22 Kazi Mazba Kamal , Alamgir Kabir

The high temperature superconductivity in single-unit-cell (1UC) FeSe on SrTiO3 (STO)(001) and the observation of replica bands by angle-resolved photoemission spectroscopy (ARPES) have led to the conjecture that the coupling between FeSe…

It is crucial to measure position and conformational changes of a membrane-interacting protein relative to the membrane surface. This is however challenging because the thickness of a membrane is usually only about 4 nm. We developed a…

Biological Physics · Physics 2018-05-29 Dong-Fei Ma , Chun-Hua Xu , Wen-Qing Hou , Chun-Yu Zhao , Lu Ma , Cong Liu , Jiajie Diao , Ying Lu , Ming Li

We study the binding of plant hormone IAA on its receptor TIR1 introducing a novel computational method that we call tomographic docking and that accounts for interactions occurring along the depth of the binding pocket. Our results suggest…

Biomolecules · Quantitative Biology 2016-10-20 Veselina V. Uzunova , Mussa Quareshy , Charo I. del Genio , Richard M. Napier

Subunit epsilon is an intrinsic regulator of the bacterial FoF1-ATP synthase, the ubiquitous membrane-embedded enzyme that utilizes a proton motive force in most organisms to synthesize adenosine triphosphate (ATP). The C-terminal domain of…

Biomolecules · Quantitative Biology 2014-02-18 Thomas M. Duncan , Monika G. Dueser , Thomas Heitkamp , Duncan G. G. McMillan , Michael Boersch

Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior in live organisms. However, detecting the response to the optical stimulation requires electrophysiology with physical…

FoF1-ATP synthases in Escherichia coli (E. coli) bacteria are membrane-bound enzymes which use an internal proton-driven rotary double motor to catalyze the synthesis of adenosine triphosphate (ATP). According to the 'chemiosmotic…

Biological Physics · Physics 2015-06-04 Marc Renz , Torsten Rendler , Michael Boersch