Related papers: Efficient wide-field FLIM
Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus…
Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…
The super-oscillation lens (SOL) can achieve super-resolution focusing but have to trade-off with weaker hotspots and higher sidebands. We propose a single compound SOL to achieve reflective confocal imaging in principle without additional…
Time-domain sampling of arbitrary electric fields with sub-cycle resolution enables a complete time-frequency analysis of a system's response to electromagnetic illumination. This provides access to dynamic information that is not provided…
Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…
Visualizing the spatiotemporal evolution of the electric field of light is fundamental to optics, from designing photonic devices to developing next-generation microscopes. However, we lack the experimental tools to directly access the…
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent…
Powerful mid-infrared illumination combined with mechanical detection via force microscopy provides access to nanoscale spectroscopic imaging in Materials and Life Sciences. Photo-induced force microscopy (PiFM) employs pulsed illumination…
Quantitatively mapping three-dimensional (3D) flow, diffusion, and particle density in crowded living cells remains challenging because most dynamic optical microscopy measurements are effectively planar and existing analysis methods…
Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…
For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial…
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…
We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…
Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…
Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…
We present feasibility study of an accelerator-based ultrafast transmission electron microscope (u-TEM) capable of producing a full field image in a single-shot with simultaneous picosecond temporal resolution and nanometer spatial…
We propose and demonstrate a photon-efficient optical classifier to overcome the Rayleigh limit in spatial resolution. It utilizes mode-selective sum frequency generation and single-pixel photon detection to resolve closely spaced…
Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…
The determination of minority-carrier lifetimes and surface recombination velocities is essential for the development of semiconductor technologies such as solar cells. The recent development of two-photon time-resolved microscopy allows…
Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…