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Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

Motivated by recent experimental initiatives, such as at the Helmholtz International Beamline for Extreme Fields (HIBEF) at the European X-ray Free Electron Laser (XFEL), we calculate the birefringent scattering of x-rays at the combined…

Advancements in digital imaging technologies have sparked increased interest in using multiplexed immunofluorescence (mIF) images to visualise and identify the interactions between specific immunophenotypes with the tumour microenvironment…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Piumi Sandarenu , Julia Chen , Iveta Slapetova , Lois Browne , Peter H. Graham , Alexander Swarbrick , Ewan K. A. Millar , Yang Song , Erik Meijering

X-ray free-electron lasers (FELs) are promising tools for structural determination of macromolecules via coherent x-ray scattering. During ultrashort and ultraintense x-ray pulses with an atomic scale wavelength, samples are subject to…

Atomic Physics · Physics 2011-10-18 Sang-Kil Son , Linda Young , Robin Santra

The requirements to achieve high detection efficiency (above 50\%) and gigahertz (GHz) frame rate for the proposed 42-keV X-ray free-electron laser (XFEL) at Los Alamos are summarized. Direct detection scenarios using C (diamond), Si, Ge…

Instrumentation and Detectors · Physics 2023-08-22 Zhehui Wang

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Compressive Macroscopic Fluorescence Lifetime Imaging (MFLI) is a novel technical implementation that enables monitoring multiple molecular interactions in macroscopic scale. Especially, we reported recently on the development of a…

Medical Physics · Physics 2018-02-23 Ruoyang Yao , Marien Ochoa , Xavier Intes , Pingkun Yan

Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…

Biological Physics · Physics 2017-11-15 Edakkattuparambil Sidharth Shibu , Nadezda Varkentina , Laurent Cognet , Brahim Lounis

Face forgery detection is raising ever-increasing interest in computer vision since facial manipulation technologies cause serious worries. Though recent works have reached sound achievements, there are still unignorable problems: a)…

Computer Vision and Pattern Recognition · Computer Science 2021-03-17 Jiaming Li , Hongtao Xie , Jiahong Li , Zhongyuan Wang , Yongdong Zhang

This paper proposes an easy-to-use method for one-class classification: Repeated Element-wise Folding (REF). The algorithm consists of repeatedly standardizing and applying an element-wise folding operation on the one-class training data.…

Machine Learning · Computer Science 2025-06-19 Jenni Raitoharju

We evaluate the performance of federated learning (FL) in developing deep learning models for analysis of digitized tissue sections. A classification application was considered as the example use case, on quantifiying the distribution of…

Image and Video Processing · Electrical Eng. & Systems 2022-04-04 Ujjwal Baid , Sarthak Pati , Tahsin M. Kurc , Rajarsi Gupta , Erich Bremer , Shahira Abousamra , Siddhesh P. Thakur , Joel H. Saltz , Spyridon Bakas

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods,…

This study introduces SEFI (SEgmentation-Free Integration), a novel method for integrating morphological features of cell nuclei with spatial transcriptomics data. Cell segmentation poses a significant challenge in the analysis of spatial…

Image and Video Processing · Electrical Eng. & Systems 2025-02-21 Eduard Chelebian , Pratiti Dasgupta , Zainalabedin Samadi , Carolina Wählby , Amjad Askary

We study a nonlinear Schr\"odinger equation which arises as an effective single particle model in X-ray Free Electron Lasers (XFEL). This equation appears as a first-principles model for the beam-matter interactions that would take place in…

Numerical Analysis · Mathematics 2014-06-18 Paolo Antonelli , Agissilaos Athanassoulis , Zhongyi Huang , Peter A. Markowich

Deep learning-based fault diagnosis (FD) approaches require a large amount of training data, which are difficult to obtain since they are located across different entities. Federated learning (FL) enables multiple clients to collaboratively…

Machine Learning · Computer Science 2023-10-16 Jixuan Cui , Jun Li , Zhen Mei , Kang Wei , Sha Wei , Ming Ding , Wen Chen , Song Guo

The proliferation of AI-generated imagery poses escalating challenges for multimedia forensics, yet many existing detectors depend on assumptions about the internals of specific generative models, limiting their cross-model applicability.…

Computer Vision and Pattern Recognition · Computer Science 2026-03-02 Nan Zhong , Mian Zou , Yiran Xu , Zhenxing Qian , Xinpeng Zhang , Baoyuan Wu , Kede Ma

In clinical applications, X-ray technology is vital for noninvasive examinations like mammography, providing essential anatomical information. However, the radiation risk associated with X-ray procedures raises concerns. X-ray…

Image and Video Processing · Electrical Eng. & Systems 2024-07-26 Tajamul Ashraf , Tisha Madame

X-ray fluorescence holography (XFH) is a method for obtaining diffraction-limited images of the local atomic structure around a given type of emitter. The reconstructed wave-field represents a distorted image of the scatterer electron…

Condensed Matter · Physics 2009-09-29 S. Marchesini , C. S. Fadley , F. J. Garcia de Abajo

Fourier Transform Interferometry (FTI) is an appealing Hyperspectral (HS) imaging modality for many applications demanding high spectral resolution, e.g., in fluorescence microscopy. However, the effective resolution of FTI is limited by…

Information Theory · Computer Science 2019-01-31 A. Moshtaghpour , L. Jacques , V. Cambareri , P. Antoine , M. Roblin

The detection and classification of exfoliated two-dimensional (2D) material flakes from optical microscope images can be automated using computer vision algorithms. This has the potential to increase the accuracy and objectivity of…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Jan-Lucas Uslu , Alexey Nekrasov , Alexander Hermans , Bernd Beschoten , Bastian Leibe , Lutz Waldecker , Christoph Stampfer