Related papers: In vivo ultrasound-switchable fluorescence imaging
Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…
In vivo fluorescence imaging in the second near-infrared window (1.0-1.7 microns) can afford deep tissue penetration and high spatial resolution, owing to the reduced scattering of long-wavelength photons. Here, we synthesize a series of…
Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…
Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…
Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay…
Background: This protocol introduces an ultrasound (US) configuration for whole-body 3D dynamic contrast-enhanced ultrasound (DCE-US) imaging in preclinical applications. The set-up relies on a clinical abdominal matrix US probe to enable…
Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron…
Ultrasound (US) imaging is better suited for intraoperative settings because it is real-time and more portable than other imaging techniques, such as mammography. However, US images are characterized by lower spatial resolution noise-like…
Developing a chip-based super-resolution imaging technique with large field-of-view (FOV), deep subwavelength resolution, and compatibility for both fluorescent and non-fluorescent samples is desired for material science, biomedicine, and…
Transcranial ultrasound imaging is usually limited by skull-induced attenuation and high-order aberrations. By using contrast agents such as microbubbles in combination with ultrafast imaging, not only can the signal-to-noise ratio be…
Compared to imaging in the visible and near-infrared regions below 900 nm, imaging in the second near-infrared window (NIR-II, 1000-1700 nm) is a promising method for deep-tissue high-resolution optical imaging in vivo mainly due to the…
Modern perception increasingly relies on fisheye, panoramic, and other wide field-of-view (FoV) cameras, yet most pipelines still apply planar CNNs designed for pinhole imagery on 2D grids, where pixel-space neighborhoods misrepresent…
Ultrasound super-localization microscopy techniques presented in the last few years enable non-invasive imaging of vascular structures at the capillary level by tracking the flow of ultrasound contrast agents (gas microbubbles). However,…
Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…
Multiple light scattering in tissue limits the penetration of optical coherence tomography (OCT) imaging. Here, we present in-vivo OCT imaging of a live mouse using wavefront shaping to enhance the penetration depth. A digital micro-mirror…
An alternative approach to ultrasound computed tomography (USCT) for medical imaging is proposed, with the intent to (i) shorten acquisition time for devices with a large number of emitters, (ii) eliminate the calibration step, and (iii)…
Near-infrared fluorescence (NIRF) can deliver high-contrast, video-rate, non-contact imaging of tumor-targeted contrast agents with the potential to guide surgeries excising solid tumors. However, it has been met with skepticism for…
To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-infrared…
Ultrasound is widely used in medical diagnostics allowing for accessible and powerful imaging but suffers from resolution limitations due to diffraction and the finite aperture of the imaging system, which restricts diagnostic use. The…
Imaging with optical resolution through highly scattering media is a long sought-after goal with important applications in deep tissue imaging. Although being the focus of numerous works, this goal was considered impractical until recently.…