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It has long been asserted that proteins like transcription factors may locate their target in DNA sequences at rates that surpass by several orders of magnitude the three-dimensional diffusion limit thank to facilitated diffusion, that is…

Biological Physics · Physics 2010-09-27 Ana-Maria Florescu , Marc Joyeux

Nucleic acids are highly deformable helical molecules constantly stretched, twisted and bent in their biological functioning. Single molecule experiments have shown that double stranded (ds)-RNA and standard ds-DNA have opposite…

Soft Condensed Matter · Physics 2023-06-28 Marco Zoli

DNA is subject to large deformations in a wide range of biological processes. Two key examples illustrate how such deformations influence the readout of the genetic information: the sequestering of eukaryotic genes by nucleosomes, and DNA…

Biomolecules · Quantitative Biology 2015-11-10 Stephanie Johnson , Martin Lindén , Rob Phillips

We establish well-posedness results for systems of a finite number of stochastic particles driven by independent Brownian motions and subject to a strongly singular drift induced by a Lennard-Jones interaction. In addition to the pairwise…

Probability · Mathematics 2026-02-16 Daniela Morale , Giulia Rui , Stefania Ugolini

Gene transcription mediated by RNA polymerase II (pol-II) is a key step in gene expression. The dynamics of pol-II moving along the transcribed region influence the rate and timing of gene expression. In this work we present a probabilistic…

The computer-aided folding of biomolecules, particularly RNAs, is one of the most difficult challenges in computational structural biology. RNA tetraloops are fundamental RNA motifs playing key roles in RNA folding and RNA-RNA and…

Biomolecules · Quantitative Biology 2016-09-28 Petra Kührová , Robert B. Best , Sandro Bottaro , Giovanni Bussi , Jiří Šponer , Michal Otyepka , Pavel Banáš

The expression of genes usually follows a two-step procedure. First, a gene (encoded in the genome) is transcribed resulting in a strand of (messenger) RNA. Afterwards, the RNA is translated into protein. Classically, this gene expression…

Molecular Networks · Quantitative Biology 2014-08-08 Martin Jansen , Peter Pfaffelhuber

Recent single-molecule pulling experiments have shown how it is possible to manipulate RNA molecules using optical tweezers force microscopy. We investigate a minimal model for the experimental setup which includes a RNA molecule connected…

Statistical Mechanics · Physics 2009-11-10 Maria Manosas , Felix Ritort

Noise-assisted transport phenomena highlight the nontrivial interplay between environmental effects and quantum coherence in achieving maximal efficiency. Due to the complexity of biochemical systems and their environments, effective open…

The dynamics of gene transcription is tightly regulated in eukaryotes. Recent experiments have revealed various kinds of transcriptional dynamics, such as RNA polymerase II pausing, that involves regulation at the transcription initiation…

Cell Behavior · Quantitative Biology 2015-08-11 Yucheng Hu , John Lowengrub

We introduce a class of distance-dependent interactions in an accelerated exclusion process (AEP) inspired by the observation of transcribing RNA polymerase speeding up when "pushed" by a trailing one. On a ring, the AEP steady state…

Statistical Mechanics · Physics 2012-10-02 Jiajia Dong , Stefan Klumpp , Royce K. P. Zia

Despite the absence of a membrane-enclosed nucleus, the bacterial DNA is typically condensed into a compact body - the nucleoid. This compaction influences the localization and dynamics of many cellular processes including transcription,…

Stochastic resonance (SR) is a well known phenomenon in dynamical systems. It consists of the amplification and optimization of the response of a system assisted by stochastic noise. Here we carry out the first experimental study of SR in…

Biological Physics · Physics 2012-12-12 K. Hayashi , S. de Lorenzo , M. Manosas , J. M. Huguet , F. Ritort

A mesoscopic model for heterogeneous DNA denaturation is developed in the framework of the path integral formalism. The base pair stretchings are treated as one-dimensional, time dependent paths contributing to the partition function. The…

Soft Condensed Matter · Physics 2011-07-12 Marco Zoli

We study the parameter sensitivity of hetero-polymeric DNA within the purview of DNA breathing dynamics. The degree of correlation between the mean bubble size and the model parameters are estimated for this purpose for three different DNA…

Biomolecules · Quantitative Biology 2014-03-27 Srijeeta Talukder , Shrabani Sen , Prantik Chakraborti , Ralf Metzler , Suman K Banik , Pinaki Chaudhury

This study uses a combination of stochastic optimization, statistical mechanical theory, and molecular simulation to test the extent to which the long-time dynamics of a single tracer particle can be enhanced by rationally modifying its…

Soft Condensed Matter · Physics 2011-11-24 James Carmer , Gaurav Goel , Mark J. Pond , Jeffrey R. Errington , Thomas M. Truskett

Nucleic acids have been regarded as stiff polymers with long-range flexibility and generally modeled using elastic rod models of polymer physics. Notwithstanding, investigations carried out over the past few years on single fragments of…

Soft Condensed Matter · Physics 2025-05-09 Marco Zoli

Gene expression consists in the synthesis of proteins from the information encoded on DNA. One of the two main steps of gene expression is the translation of messenger RNA (mRNA) into polypeptide sequences of amino acids. Here, by taking…

Biomolecular condensates have been shown to play a fundamental role in localizing biochemistry in a cell. RNA is a common constituent of condensates, and can determine their biophysical properties. Functions of biomolecular condensates are…

Subcellular Processes · Quantitative Biology 2023-02-22 Alex Mayer , Grace McLaughlin , Sierra Cole , Amy Gladfelter , Marcus Roper

Accurate knowledge of RNA hybridization is essential for understanding RNA structure and function. Here we mechanically unzip and rezip a 2-kbp RNA hairpin and derive the 10 nearest-neighbor base pair (NNBP) RNA free energies in sodium and…

Biomolecules · Quantitative Biology 2022-01-14 Paolo Rissone , Cristiano Valim Bizarro , Felix Ritort