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We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

In deep tissue photoacoustic imaging, the spatial resolution is inherently limited by acoustic diffraction. Moreover, as the ultrasound attenuation increases with frequency, resolution is often traded-off for penetration depth. Here we…

Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

We present a novel method to synthesize non-trivial speckles that can enable superresolving second-order correlation imaging. The speckles acquire a unique anti-correlation in the spatial intensity fluctuation by introducing the blue noise…

We report on image processing techniques and experimental procedures to determine the lattice-site positions of single atoms in an optical lattice with high reliability, even for limited acquisition time or optical resolution. Determining…

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

By switching fluorophores on and off in either a deterministic or a stochastic manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical…

Super-resolution reconstruction techniques entail the utilization of software algorithms to transform one or more sets of low-resolution images captured from the same scene into high-resolution images. In recent years, considerable…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Hao Yan , Zixiang Wang , Zhengjia Xu , Zhuoyue Wang , Zhizhong Wu , Ranran Lyu

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes…

Biological Physics · Physics 2015-06-16 Bin Yang , Frédéric Przybilla , Michael Mestre , Jean-Baptiste Trebbia , Brahim Lounis

With the evolving technology in CMOS integration, new classes of 2D-imaging detectors have recently become available. In particular, single photon avalanche diode (SPAD) arrays allow detection of single photons at high acquisition rates…

Bolton and Schlegel presented a promising deconvolution method to extract 1D spectra from a 2D optical fiber spectral CCD image. The method could eliminate the PSF difference between fibers, extract spectra to the photo noise level, as well…

Instrumentation and Methods for Astrophysics · Physics 2015-06-24 Guangwei Li , Haotong Zhang , Zhongrui Bai

Digitising the 3D world into a clean, CAD model-based representation has important applications for augmented reality and robotics. Current state-of-the-art methods are computationally intensive as they individually encode each detected…

Computer Vision and Pattern Recognition · Computer Science 2024-03-25 Florian Langer , Jihong Ju , Georgi Dikov , Gerhard Reitmayr , Mohsen Ghafoorian

Recently, super-resolution ultrasound imaging with ultrasound localization microscopy (ULM) has received much attention. However, ULM relies on low concentrations of microbubbles in the blood vessels, ultimately resulting in long…

We present a new method to achieve high-contrast images using segmented and/or on-axis telescopes. Our approach relies on using two sequential Deformable Mirrors to compensate for the large amplitude excursions in the telescope aperture due…

Instrumentation and Methods for Astrophysics · Physics 2015-06-12 Laurent Pueyo , Colin Norman

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

One of the main limitations for the resolution of optical instruments is the size of the sensor's pixels. In this paper we introduce a new sub pixel resolution algorithm to enhance the resolution of images. This method is based on the…

Instrumentation and Detectors · Physics 2012-11-12 Siamak Khademi , Ahmad Darudi , Zahra Abbasi

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat