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Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up. With modern instrumentation this limitation can be lifted significantly but some…

Data Analysis, Statistics and Probability · Physics 2018-05-23 Matthias Patting , Paja Reisch , Marcus Sackrow , Rhys Dowler , Marcelle Koenig , Michael Wahl

Time-Correlated Single Photon Counting (TCSPC) and time tagging of individual photon detections are powerful tools in many quantum optical experiments and other areas of applied physics. Using TCSPC, e.g., for the purpose of fluorescence…

Instrumentation and Detectors · Physics 2020-01-09 Michael Wahl , Tino Roehlicke , Sebastian Kulisch , Sumeet Rohilla , Benedikt Kraeamer , Andreas C. Hocke

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or…

Detection of radiation signals is at the heart of precision metrology and sensing. In this article we show how the fluctuations in photon counting signals can be exploited to optimally extract information about the physical parameters that…

Quantum Physics · Physics 2014-05-13 Alexander Holm Kiilerich , Klaus Mølmer

We present a statistics-aware compression strategy that processes photon timestamps directly from time-correlated single-photon counting (TCSPC) modules for time-domain fluorescence lifetime imaging (FLIM). Rather than storing or…

Image and Video Processing · Electrical Eng. & Systems 2026-05-08 Zhenya Zang , Istvan Gyongy , Mike Davies

We present a Fisher-information (FI) framework for photon-efficient fluorescence lifetime imaging microscopy (FLIM) that treats temporal sampling as a controllable design variable under a fixed photon (dose) budget. Starting from a Poisson…

Instrumentation and Detectors · Physics 2026-01-05 J. Sumaya-Martinez , E. Torres-Garcia

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

Precise diagnostic on the electron beam parameters is a very valuable tool and essential in the operation of synchrotron light sources. One possible option is to employ the emitted synchrotron radiation for non-destructive measurements. A…

Accelerator Physics · Physics 2025-12-08 Miriam Brosi , Johann Schmand , Jonas Breunlin , Francesca Curbis

Assessing the quality of parameter estimates for models describing the motion of single molecules in cellular environments is an important problem in fluorescence microscopy. We consider the fundamental data model, where molecules emit…

Quantitative Methods · Quantitative Biology 2021-09-15 A. Marie d'Avigneau , S. S. Singh , R. J. Ober

Complex frequency excitations, oscillating signals whose amplitude decreases exponentially in time, have recently been demonstrated to significantly increase the effective quality factor of mechanical resonators. In this work, we…

Classical Physics · Physics 2026-05-14 Wenbo Li , Raj Kumar Pal

Time-resolved photon-counting plays an indispensable role in precision metrology in both classical and quantum regimes. In particular, time-correlated single-photon counting (TCSPC) has been the key enabling technology for applications such…

Optics · Physics 2021-10-29 Bowen Li , Jan Bartos , Yijun Xie , Shu-Wei Huang

This paper presents a new Bayesian model and algorithm used for depth and intensity profiling using full waveforms from the time-correlated single photon counting (TCSPC) measurement in the limit of very low photon counts. The model…

Applications · Statistics 2016-04-20 Yoann Altmann , Ximing Ren , Aongus McCarthy , Gerald S. Buller , Steve McLaughlin

We describe a compact and reliable method to calculate the Fisher information for the estimation of a dynamical parameter in a continuously measured linear Gaussian quantum system. Unlike previous methods in the literature, which involve…

Quantum Physics · Physics 2017-06-08 Marco G. Genoni

Precision measurement has been an important research area in sensing and metrology. In classical physics, the Fisher information determines the maximum extractable information from statistically unknown signals, based on a joint probability…

Quantum Physics · Physics 2024-09-04 Byoung S. Ham

Precision measurements with quantum systems rely on our ability to trace the differences between experimental signals to variations in unknown physical parameters. In this Letter we derive the Fisher information and the ensuing Cramer-Rao…

Quantum Physics · Physics 2015-06-17 Søren Gammelmark , Klaus Mølmer

Fluorescence lifetime imaging (FLI) has been receiving increased attention in recent years as a powerful diagnostic technique in biological and medical research. However, existing FLI systems often suffer from a tradeoff between processing…

Image and Video Processing · Electrical Eng. & Systems 2025-11-10 Yang Lin , Paul Mos , Andrei Ardelean , Claudio Bruschini , Edoardo Charbon

The prequel to this work [Ng et al., Phys. Rev. A 93, 042121 (2016)] proposes the method of spectral photon counting to enhance noise spectroscopy with an optical interferometer. While the predicted enhancement over homodyne detection is…

Quantum Physics · Physics 2026-04-14 Xinyi Sui , Mankei Tsang

In this paper we use the Cramer-Rao lower uncertainty bound to estimate the maximum precision that could be achieved on the joint simultaneous (or 2D) estimation of photometry and astrometry of a point source measured by a linear CCD…

Instrumentation and Methods for Astrophysics · Physics 2015-06-22 Rene A. Mendez , Jorge F. Silva , Rodrigo Orsotica , Rodrigo Lobos

Enhancing the precision of a measurement requires maximizing the information that can be gained about the quantity of interest from probing a system. For optical based measurements, such an enhancement can be achieved through two…

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