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In many laboratories, conventional bright-field transmission microscopes are available to study the structure and organization principles of fibrous tissue samples, but they usually provide only 2D information. To access the third…

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

The pressure and flow statistics of Darcy flow through a random permeable medium are expressed in a form suitable for evaluation by the method of simulated annealing. There are several attractive aspects to using simulated annealing: (i)…

Computational Engineering, Finance, and Science · Computer Science 2019-04-04 Marise J. E. Westbroek , Peter R. King , Dimitri D. Vvedensky , Ronnie L. Schwede

The study performs large-eddy simulations of supersonic free jet flows using the Discontinuous Galerkin Spectral Element Method (DGSEM). The main objective of the present work is to assess the resolution requirements for adequate simulation…

Fluid Dynamics · Physics 2024-03-22 Diego F. Abreu , João Luiz F. Azevedo , Carlos Junqueira-Junior

Modeling the evolution of high-dimensional systems from limited snapshot observations at irregular time points poses a significant challenge in quantitative biology and related fields. Traditional approaches often rely on dimensionality…

Machine Learning · Computer Science 2025-08-07 Justin Lee , Behnaz Moradijamei , Heman Shakeri

We report on a flow sensing approach based on deflection monitoring of micro beams buckled by the compressive thermal stress due to electrothermal Joules heating. The air stream convectively cooling the device affects both the critical…

Fluid Dynamics · Physics 2016-09-21 Yoav Kessler , Slava Krylov , Alex Liberzon

Recent microfluidic experiments have explored the precise positioning of micron-sized particles in liquid environments via laser-induced thermoviscous flow. From micro-robotics to biology at the subcellular scale, this versatile technique…

Fluid Dynamics · Physics 2025-10-01 Weida Liao , Eric Lauga

Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon-efficiency, field of view, mechanical complexity, spectral…

The circulatory system, comprising the heart and blood vessels, is vital for nutrient transport, waste removal, and homeostasis. Traditional computational models often treat cardiac electromechanics and blood flow dynamics separately,…

Electronic conduction pathways in dielectric thin films are explored using automated experiments in scanning probe microscopy (SPM). Here, we use large field of view scanning to identify the position of localized conductive spots and…

Accessing and characterizing a flow impose a number of constraints on the employed measurement techniques; in particular optical methods require transparent fluids and windows in the vessel. Whereas one can adapt apparatus, fluid and…

Recent developments in vortex particle methods for simulating three-dimensional incompressible flows are presented. A lightweight, dynamic Large-Eddy Simulation model is tested, featuring a dynamic procedure that relies solely on Lagrangian…

Fluid Dynamics · Physics 2026-01-13 Flavio A. C. Martins , Alexander van Zuijlen , Carlos J. Simao Ferreira

The dynamics of solute flow in the microscopic chamber can be studied with optical tweezers. A method based on the metallic microbeads trapped in the focused optical vortex beam is proposed. This annular beam of a twisted wavefront exerts…

Soft Condensed Matter · Physics 2023-01-11 Weronika Lamperska , Jan Masajada , Sławomir Drobczyński

Image-based computational fluid dynamics (CFD) has emerged as a powerful tool to study cardiovascular flows while 2D echocardiography (echo) is the most widely used non-invasive imaging modality for diagnosis of heart disease. Here, echo is…

This study extends the flow measurement method initially proposed in [22] to three-dimensional scenarios, addressing the growing need for accurate and efficient non-contact flow measurement techniques in complex hydrodynamic environments.…

Numerical Analysis · Mathematics 2025-03-18 Jiwei Li , Lingyun Qiu , Zhongjing Wang , Hui Yu

We use confocal microscopy to directly visualize the spatial fluctuations in fluid flow through a three-dimensional porous medium. We find that the velocity magnitudes and the velocity components both along and transverse to the imposed…

Soft Condensed Matter · Physics 2013-08-07 Sujit S. Datta , Harry Chiang , T. S. Ramakrishnan , David A. Weitz

Flow cytometry is a technique that measures multiple fluorescence and light scatter-associated parameters from individual cells as they flow a single file through an excitation light source. These cells are labeled with antibodies to detect…

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Determining brain hemodynamics plays a critical role in the diagnosis and treatment of various cerebrovascular diseases. In this work, we put forth a physics-informed deep learning framework that augments sparse clinical measurements with…

Medical Physics · Physics 2021-08-27 Mohammad Sarabian , Hessam Babaee , Kaveh Laksari
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