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Phase microscopy is an invaluable tool in the biosciences and in clinical diagnostics. The sensitivity of current phase microscopy techniques is optimized for one specific mean phase value and varies significantly across a given sample.…
Retrieving object phase from the optical fringe pattern is a critical task in quantitative phase imaging and often requires appropriate image preprocessing (background and noise minimization), especially when retrieving phase from the…
The Frozen Section (FS) technique is a rapid and efficient method, taking only 15-30 minutes to prepare slides for pathologists' evaluation during surgery, enabling immediate decisions on further surgical interventions. However, FS process…
In this paper we propose a very efficient method to fuse the unregistered multi-focus microscopical images based on the speed-up robust features (SURF). Our method follows the pipeline of first registration and then fusion. However, instead…
Photoacoustic (PA) endoscopy has shown significant potential for clinical diagnosis and surgical guidance. Multimode fibres (MMFs) are becoming increasing attractive for the development of miniature endoscopy probes owing to ultrathin size,…
The expanding adoption of digital pathology has enabled the curation of large repositories of histology whole slide images (WSIs), which contain a wealth of information. Similar pathology image search offers the opportunity to comb through…
Significance: Histopathological analysis of tissues is an essential tool for grading, staging, diagnosing and resecting cancers and other malignancies. Current histopathological techniques require substantial sample processing prior to…
The overexpression of human epidermal growth factor receptor 2 (HER2) has been established as a therapeutic target in multiple types of cancers, such as breast and gastric cancers. Immunohistochemistry (IHC) is employed as a basic HER2 test…
Micro-fluidic pumps as well as artificial micro-swimmers are conveniently realized exploiting phoretic solvent flows based on local gradients of temperature, electrolyte concentration or pH. We here present a facile micro-photometric method…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
We present an ultra-thin hybrid imaging system based on an optical multimode fiber (MMF) and an optical fiber hydrophone that combines optical resolution photoacoustic and fluorescence microscopy. To control the illumination at the distal…
We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…
Accurate assessment of fish freshness remains a major challenge in the food industry, with direct consequences for product quality, market value, and consumer health. Conventional sensory evaluation is inherently subjective, inconsistent,…
Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…
The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely…
Nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence in situ hybridization (FISH) microscopy provide high-resolution, multimodal image representations of the identity and cell activity respectively of targeted microbial…
Tissue oxygenation and perfusion can be an indicator for organ viability during minimally invasive surgery, for example allowing real-time assessment of tissue perfusion and oxygen saturation. Multispectral imaging is an optical modality…
Multiplex immunofluorescence (MxIF) is an emerging imaging technique that produces the high sensitivity and specificity of single-cell mapping. With a tenet of 'seeing is believing', MxIF enables iterative staining and imaging extensive…
A fundamental issue in biomedical and environmental sciences is the development of sensitive and robust sensors able to probe the analyte of interest, under physiological and pathological conditions or in environmental samples, and with…
Rapid identification of microparticles in liquid is an important problem in environmental and biomedical applications such as for microplastic detection in water sources and physiological fluids. Existing spectro-scopic techniques are…