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Cell instance segmentation in fluorescence microscopy images is becoming essential for cancer dynamics and prognosis. Data extracted from cancer dynamics allows to understand and accurately model different metabolic processes such as…

Star-shaped bodies are an important nonconvex generalization of convex bodies (e.g., linear programming with violations). Here we present an efficient algorithm for sampling a given star-shaped body. The complexity of the algorithm grows…

Data Structures and Algorithms · Computer Science 2009-04-06 Karthekeyan Chandrasekaran , Daniel Dadush , Santosh Vempala

Microscopy imaging techniques are instrumental for characterization and analysis of biological structures. As these techniques typically render 3D visualization of cells by stacking 2D projections, issues such as out-of-plane excitation and…

Image and Video Processing · Electrical Eng. & Systems 2023-02-16 Amirkoushyar Ziabari , Derek C. Rose , Abbas Shirinifard , David Solecki

The segmentation of cell nuclei in tissue images stained with the blood dye hematoxylin and eosin (H$\&$E) is essential for various clinical applications and analyses. Due to the complex characteristics of cellular morphology, a large…

Image and Video Processing · Electrical Eng. & Systems 2024-07-26 Ziwei Cui , Jingfeng Yao , Lunbin Zeng , Juan Yang , Wenyu Liu , Xinggang Wang

Automation-assisted cervical screening via Pap smear or liquid-based cytology (LBC) is a highly effective cell imaging based cancer detection tool, where cells are partitioned into "abnormal" and "normal" categories. However, the success of…

Computer Vision and Pattern Recognition · Computer Science 2018-01-29 Ling Zhang , Le Lu , Isabella Nogues , Ronald M. Summers , Shaoxiong Liu , Jianhua Yao

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…

Computer Vision and Pattern Recognition · Computer Science 2019-01-08 Assaf Arbelle , Tammy Riklin Raviv

Lung cancer is the most common form of cancer found worldwide with a high mortality rate. Early detection of pulmonary nodules by screening with a low-dose computed tomography (CT) scan is crucial for its effective clinical management.…

Image and Video Processing · Electrical Eng. & Systems 2020-06-17 Rakshith Sathish , Rachana Sathish , Ramanathan Sethuraman , Debdoot Sheet

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

Image and Video Processing · Electrical Eng. & Systems 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

This paper introduces a novel deep-learning method for the automatic detection and segmentation of lung nodules, aimed at advancing the accuracy of early-stage lung cancer diagnosis. The proposed approach leverages a unique "Channel Squeeze…

Image and Video Processing · Electrical Eng. & Systems 2024-09-24 Mingxiu Sui , Jiacheng Hu , Tong Zhou , Zibo Liu , Likang Wen , Junliang Du

Accurately and quickly binuclear cell (BC) detection plays a significant role in predicting the risk of leukemia and other malignant tumors. However, manual microscopy counting is time-consuming and lacks objectivity. Moreover, with the…

Computer Vision and Pattern Recognition · Computer Science 2022-08-29 Baomin Wang , Geng Hu , Dan Chen , Lihua Hu , Cheng Li , Yu An , Guiping Hu , Guang Jia

We present an algorithm for multi-scale tumor (chimeric cell) detection in high resolution slide scans. The broad range of tumor sizes in our dataset pose a challenge for current Convolutional Neural Networks (CNN) which often fail when…

Computer Vision and Pattern Recognition · Computer Science 2021-04-27 Qingchao Zhang , Coy D. Heldermon , Corey Toler-Franklin

Traditional breast cancer image classification methods require manual extraction of features from medical images, which not only require professional medical knowledge, but also have problems such as time-consuming and labor-intensive and…

Image and Video Processing · Electrical Eng. & Systems 2021-04-26 Mengfan Li

Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important. Here we apply a…

Neurons and Cognition · Quantitative Biology 2016-12-23 Noah J. Apthorpe , Alexander J. Riordan , Rob E. Aguilar , Jan Homann , Yi Gu , David W. Tank , H. Sebastian Seung

We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

The progress in imaging techniques have allowed the study of various aspect of cellular mechanisms. To isolate individual cells in live imaging data, we introduce an elegant image segmentation framework that effectively extracts cell…

Computer Vision and Pattern Recognition · Computer Science 2016-02-18 Arnaud Browet , Christophe De Vleeschouwer , Laurent Jacques , Navrita Mathiah , Bechara Saykali , Isabelle Migeotte

Two successful approaches for the segmentation of biomedical images are (1) the selection of segment candidates from a merge-tree, and (2) the clustering of small superpixels by solving a Multi-Cut problem. In this paper, we introduce a…

Computer Vision and Pattern Recognition · Computer Science 2017-07-05 Jan Funke , Chong Zhang , Tobias Pietzsch , Stephan Saalfeld

The advancement of the neuroscientific imaging techniques has produced an unprecedented size of neural cell imaging data, which calls for automated processing. In particular, identification of cells from two photon images demands…

Image and Video Processing · Electrical Eng. & Systems 2019-09-26 Si-Baek Seong , Hae-Jeong Park

In digital pathology, both detection and classification of cells are important for automatic diagnostic and prognostic tasks. Classifying cells into subtypes, such as tumor cells, lymphocytes or stromal cells is particularly challenging.…

Computer Vision and Pattern Recognition · Computer Science 2022-06-07 Shahira Abousamra , David Belinsky , John Van Arnam , Felicia Allard , Eric Yee , Rajarsi Gupta , Tahsin Kurc , Dimitris Samaras , Joel Saltz , Chao Chen

Reliable cell segmentation and classification from biomedical images is a crucial step for both scientific research and clinical practice. A major challenge for more robust segmentation and classification methods is the large variations in…

Cell Behavior · Quantitative Biology 2017-10-31 Mo Zhang , Xiang Li , Mengjia Xu , Quanzheng Li

We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha