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Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with…

Quantitative Methods · Quantitative Biology 2024-12-13 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

Optics · Physics 2024-11-18 Doron Shterman , Guy Bartal

Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

Computer Vision and Pattern Recognition · Computer Science 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

Light-field microscopy (LFM) is a 3D microscopy technique whereby volumetric information of a sample is gained by simultaneously capturing both the position and momentum (angular) information of light illuminating a scene. Conventional LFM…

Quantum Physics · Physics 2024-03-01 Yingwen Zhang , Duncan England , Antony Orth , Ebrahim Karimi , Benjamin Sussman

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

In a lens-assisted imaging scheme with speckle illumination, the spatial resolution can surpass the Rayleigh resolution limit by a factor of $\sqrt{2}$ with second-order auto-correlation of light intensity. In this work, integrated with the…

Optics · Physics 2024-07-31 Liming Li

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

We present a novel method to synthesize non-trivial speckles that can enable superresolving second-order correlation imaging. The speckles acquire a unique anti-correlation in the spatial intensity fluctuation by introducing the blue noise…

An important topic of interest in imaging is the construction of protocols that are not diffraction limited. This can be achieved in a variety of ways, including classical superresolution techniques or quantum entanglement-based protocols.…

Quantum Physics · Physics 2015-10-28 Mark E. Pearce , Thomas Mehringer , J. von Zanthier , Pieter Kok

Correlation plenoptic imaging (CPI) is a scanning-free diffraction-limited 3D optical imaging technique exploiting the peculiar properties of correlated light sources. CPI has been further extended to samples of interest to microscopy, such…

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

Optics · Physics 2022-03-09 James D. Manton