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We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim

Imaging nuclei of keratinocytes in the stratified squamous epithelium has been a subject of intense research since nucleus associated cellular atypia is the key criteria for the screening and diagnosis of epithelial cancers and their…

Tissues and Organs · Quantitative Biology 2019-06-04 Si Chen , Xinyu Liu , Nanshuo Wang , Qianshan Ding , Xianghong Wang , Xin Ge , En Bo , Xiaojun Yu , Honggang Yu , Chenjie Xu , Linbo Liu

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the…

Quantitative Methods · Quantitative Biology 2018-04-27 Paul Müller , Mirjam Schürmann , Salvatore Girardo , Gheorghe Cojoc , Jochen Guck

We discuss some differences and similarities between electron and neutrino scattering off atomic nuclei. We find that, in the giant resonance region, the two processes excite different nuclear modes, therefore the weak and the…

Nuclear Theory · Physics 2009-11-10 A. Botrugno , G. Co'

We use photothermal induced resonance (PTIR) imaging and spectroscopy, in resonant and non-resonant mode, to study the cytoplasmic membrane and surface of intact cells. Non-resonant PTIR images apparently provide rich details of the cell…

Applied Physics · Physics 2021-01-27 Luca Quaroni

Accurate subtyping of renal cell carcinoma (RCC) is of crucial importance for understanding disease progression and for making informed treatment decisions. New discoveries of significant alterations to mitochondria between subtypes make…

Machine Learning · Computer Science 2016-08-03 Peter J. Schüffler , Judy Sarungbam , Hassan Muhammad , Ed Reznik , Satish K. Tickoo , Thomas J. Fuchs

Cytological diagnosis of follicular thyroid carcinoma (FTC) is one of major challenges in the field of endocrine oncology due to absence of evident morphological indicators. Morphological abnormalities in the nucleus are typically key…

Differences in the charge radii of mirror nuclei have been recently suggested to contain information on the slope of the symmetry energy L. To test this hypothesis, we perform statistical correlation analysis using quantified energy density…

Nuclear Theory · Physics 2022-02-16 Paul-Gerhard Reinhard , Witold Nazarewicz

The recent high-resolution measurement of the electric dipole (E1) polarizability (alphad) in 208Pb [Phys. Rev. Lett. 107, 062502 (2011)] provides a unique constraint on the neutron-skin thickness of this nucleus. The neutron-skin thickness…

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Neutron-rich nuclei provide important insights to nuclear forces and to the nuclear equation of state. Advances in ab initio methods combined with new opportunities with rare isotope beams enable unique explorations of their properties…

Nuclear Theory · Physics 2024-01-15 P. Arthuis , K. Hebeler , A. Schwenk

This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically…

Optics · Physics 2017-05-11 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

A key optical parameter characterizing the existence of negative refraction in a thin layer of a composite material is the effective refractive index of an equivalent, homogenized layer with the same physical thickness as the initial…

The connection between the properties of cell tissue and those of the single constituent cells remains to be elucidated. At the purely mechanical level, the degree of rigidity of different cellular components, such as the nucleus and the…

Soft Condensed Matter · Physics 2022-03-15 Stefano Villa , Andrea Palamidessi , Emanuela Frittoli , Giorgio Scita , Roberto Cerbino , Fabio Giavazzi

For sufficiently wide resonances, nuclear resonance fluorescence behaves like elastic photo-nuclear scattering while retaining the large cross-section characteristic of resonant photo-nuclear absorption. We show that NRF may be used to…

Instrumentation and Methods for Astrophysics · Physics 2013-10-18 Tenzing H. Y. Joshi

For one-neutron halo nuclei, the cross section for elastic scattering and breakup at intermediate energy exhibit similar angular dependences. The Recoil Excitation and Breakup (REB) model of reactions elegantly explains this feature. It…

Nuclear Theory · Physics 2020-12-22 Pierre Capel , Ronald C. Johnson , Filomena M. Nunes

Investigations on diverse particulate matter can be carried out by non-invasive and non-destructive analytical means of light scattering technique. Exploration of small particles using this tool must address the concept of refractive index…

A novel optical technique for measuring the complex refractive index (CRI) of photoactive proteins over the wide range of visible wavelengths is presented. Employing quantitative phase microscopy equipped with a wavelength swept source,…

Chemical Physics · Physics 2015-07-03 KyeoReh Lee , Youngmin Kim , JaeHwang Jung , Hyotcherl Ihee , YongKeun Park
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