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Focused Ion Beam Scanning Electron Microscope (FIB-SEM) imaging is a technique that image materials section-by-section at nano-resolution, e.g.,5 nanometer width voxels. FIB-SEM is well suited for imaging ultrastructures in cells.…

Quantitative Methods · Quantitative Biology 2020-08-06 Hans Jacob Teglbjærg Stephensen , Sune Darkner , Jon Sporring

Precession of a converged beam during acquisition of a 4D-STEM dataset improves strain, orientation, and phase mapping accuracy by averaging over continuous angles of illumination. Precession experiments usually rely on integrated systems,…

Instrumentation and Detectors · Physics 2025-10-20 Stephanie M. Ribet , Rohan Dhall , Colin Ophus , Karen C. Bustillo

We report on a scanning microscopy technique for atom-number-resolved imaging of excited-state atoms. A tightly focused laser beam leads to local autoionization, and the resulting ions are counted electronically. Scanning the beam across…

Atomic Physics · Physics 2015-06-12 G. Lochead , D. Boddy , D. P. Sadler , C. S. Adams , M. P. A. Jones

Scanning Electron Microscopes (SEMs) are widely renowned for their ability to analyze the surface structures of microscopic objects, offering the capability to capture highly detailed, yet only grayscale, images. To create more expressive…

Computer Vision and Pattern Recognition · Computer Science 2024-10-30 Takuma Nishimura , Andreea Dogaru , Martin Oeggerli , Bernhard Egger

In the domain of battery research, the processing of high-resolution microscopy images is a challenging task, as it involves dealing with complex images and requires a prior understanding of the components involved. The utilization of deep…

Computer Vision and Pattern Recognition · Computer Science 2025-03-19 Ganesh Raghavendran , Bing Han , Fortune Adekogbe , Shuang Bai , Bingyu Lu , William Wu , Minghao Zhang , Ying Shirley Meng

This paper presents a systematic solution for the intelligent recognition and automatic analysis of microscopy images. We developed a data engine that generates high-quality annotated datasets through a combination of the collection of…

Image and Video Processing · Electrical Eng. & Systems 2025-08-27 Yanhui Hong , Nan Wang , Zhiyi Xia , Haoyi Tao , Xi Fang , Yiming Li , Jiankun Wang , Peng Jin , Xiaochen Cai , Shengyu Li , Ziqi Chen , Zezhong Zhang , Guolin Ke , Linfeng Zhang

Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…

Materials Science · Physics 2011-12-15 Robert Hovden , Huolin L. Xin , David A. Muller

We propose a straightforward yet highly effective few-shot fine-tuning strategy for adapting the Segment Anything (SAM) to anatomical segmentation tasks in medical images. Our novel approach revolves around reformulating the mask decoder…

Computer Vision and Pattern Recognition · Computer Science 2024-07-08 Weiyi Xie , Nathalie Willems , Shubham Patil , Yang Li , Mayank Kumar

In a scanning transmission electron microscope (STEM), producing a high-resolution image generally requires an electron beam focused to the smallest point possible. However, the magnetic lenses used to focus the beam are unavoidably…

The burgeoning field of atomic level material control holds great promise for future breakthroughs in quantum and memristive device manufacture and fundamental studies of atomic-scale chemistry. Realization of atom-by atom control of matter…

Materials Science · Physics 2018-08-06 Ondrej Dyck , Songkil Kim , Sergei V. Kalinin , Stephen Jesse

Quantitative high-resolution transmission electron microscopy (HRTEM) provides an indispensable means to understand the structure-property relationships of a material in atomic dimensions. Successful quantification requires reliable…

Computational Physics · Physics 2026-03-27 Xiankang Tang , Yixuan Zhang , Juri Barthel , Chun-Lin Jia , Rafal E. Dunin-Borkowski , Hongbin Zhang , Lei Jin

We show that radiation damage to unstained biological specimens is not an intractable problem in electron microscopy. When a structural hypothesis of a specimen is available, quantum mechanical principles allow us to verify the hypothesis…

Quantum Physics · Physics 2015-07-01 Hiroshi Okamoto , Tatiana Latychevskaia , Hans-Werner Fink

In the scanning transmission electron microscope, fast-scanning and frame-averaging are two widely used approaches for reducing electron-beam damage and increasing image signal-noise ratio which require no additional specialised hardware.…

Instrumentation and Detectors · Physics 2022-01-13 Tiarnan Mullarkey , Jonathan J. P. Peters , Clive Downing , Lewys Jones

Image segmentation is a critical enabler for tasks ranging from medical diagnostics to autonomous driving. However, the correct segmentation semantics - where are boundaries located? what segments are logically similar? - change depending…

Cryo-electron microscopy (cryo-EM) studies using single particle reconstruction are extensively used to reveal structural information on macromolecular complexes. Aiming at the highest achievable resolution, state of the art electron…

Efficient capture and detection of minute amount of deoxyribonucleic acid (DNA) molecules are pivotal for an array of modern gene technologies which are widely utilized in medical, forensic and defense applications, including DNA…

Biological Physics · Physics 2018-11-19 Jianhe Guo , Kwanoh Kim , Peter Vandeventer , Donglei , Fan

Atom segmentation and localization, noise reduction and deblurring of atomic-resolution scanning transmission electron microscopy (STEM) images with high precision and robustness is a challenging task. Although several conventional…

Materials Science · Physics 2021-02-23 Ruoqian Lin , Rui Zhang , Chunyang Wang , Xiao-Qing Yang , Huolin L. Xin

Traditional beamforming of medical ultrasound images relies on sampling rates significantly higher than the actual Nyquist rate of the received signals. This results in large amounts of data to store and process, imposing hardware and…

Image and Video Processing · Electrical Eng. & Systems 2021-11-09 Alon Mamistvalov , Ariel Amar , Naama Kessler , Yonina C. Eldar

Momentum resolved 4D-STEM, also called center of mass (CoM) analysis, has been used to measure the long range built-in electric field of a silicon p-n junction. The effect of different STEM modes and the trade-off between spatial resolution…

Electromagnetic waves possessing orbital angular momentum (OAM) are powerful tools for applications in optical communications, new quantum technologies and optical tweezers. Recently, they have attracted growing interest since they can be…