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Ribosome-targeting antibiotics, such as chloramphenicol, stall elongating ribosomes during protein synthesis, disrupting mRNA translation. These antibiotic-induced pauses occur stochastically, alter collective ribosome dynamics and…

Subcellular Processes · Quantitative Biology 2025-07-28 Johannes Keisers , Norbert Kern , Luca Ciandrini

Many ribosomes simultaneously move on the same messenger RNA (mRNA), each separately synthesizing the protein coded by the mRNA. Earlier models of ribosome traffic represent each ribosome by a ``self-propelled particle'' and capture the…

Biological Physics · Physics 2009-11-11 Aakash Basu , Debashish Chowdhury

The translation of messenger RNA transcripts to proteins is commonly modeled as a one-dimensional totally asymmetric exclusion process with extended particles. Here we focus on the effects of premature termination of translation through the…

Subcellular Processes · Quantitative Biology 2023-08-31 Johannes Keisers , Joachim Krug

Many {\it ribosomes} simultaneously move on the same messenger RNA (mRNA), each synthesizing separately a copy of the same protein. In contrast to the earlier models, here {\it we develop a ``unified'' theoretical model} that not only…

Biological Physics · Physics 2007-05-23 Aakash Basu , Debashish Chowdhury

The ribosome flow model (RFM) is a phenomenological model for the flow of particles along a 1D chain of $n$ sites. It has been extensively used to study ribosome flow along the mRNA molecule during translation. When the transition rates…

Optimization and Control · Mathematics 2025-05-20 Ron Ofir , Thomas Kriecherbauer , Lars Grüne , Michael Margaliot

In this paper we study an important global regulation mechanism of transcription of biological cells using specific macro-molecules, 6S RNAs. The functional property of 6S RNAs is of blocking the transcription of RNAs when the environment…

Probability · Mathematics 2022-08-22 Vincent Fromion , Philippe Robert , Jana Zaherddine

Translation is a key step in the synthesis of proteins. Accordingly, cells have evolved an intricate array of control mechanisms to regulate this process. By constructing a multi-component mathematical framework for translation we uncover…

Biological Physics · Physics 2019-12-04 Liang Wang , M. Carmen Romano , Fordyce A. Davidson

The eukaryotic protein synthesis process entails intricate stages governed by diverse mechanisms to tightly regulate translation. Translational regulation during stress is pivotal for maintaining cellular homeostasis, ensuring the accurate…

Molecular Networks · Quantitative Biology 2024-05-06 Dilawar Ahmad Mir , Zhengxin Ma , Jordan Horrocks , Aric N Rogers

In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic…

Subcellular Processes · Quantitative Biology 2008-11-24 Stefan Klumpp , Terence Hwa

In this paper we analyze the equilibrium properties of a large class of stochastic processes describing the fundamental biological process within bacterial cells, {\em the production process of proteins}. Stochastic models classically used…

Molecular Networks · Quantitative Biology 2019-10-17 Philippe Robert

Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as…

Biological Physics · Physics 2011-01-28 Ajeet K. Sharma , Debashish Chowdhury

The Ribosome Flow Model (RFM) describes the unidirectional movement of interacting particles along a one-dimensional chain of sites. As a site becomes fuller, the effective entry rate into this site decreases. The RFM has been used to model…

Networking and Internet Architecture · Computer Science 2015-08-26 Yoram Zarai , Oz Mendel , Michael Margaliot

In the process of translation, ribosomes read the genetic code on an mRNA and assemble the corresponding polypeptide chain. The ribosomes perform discrete directed motion which is well modeled by a totally asymmetric simple exclusion…

Biomolecules · Quantitative Biology 2007-08-02 J. J. Dong , B. Schmittmann , R. K. P. Zia

The ribosome flow model (RFM) is a phenomenological model for the unidirectional flow of particles along a 1D chain of $n$ sites. The RFM has been extensively used to study the dynamics of ribosome flow along a single mRNA molecule during…

Molecular Networks · Quantitative Biology 2026-01-09 Aliza Ehrman , Thomas Kriecherbauer , Lars Gruene , Michael Margaliot

Messenger RNA encodes a sequence of amino acids by using codons. For most amino acids there are multiple synonymous codons that can encode the amino acid. The translation speed can vary from one codon to another, thus there is room for…

Subcellular Processes · Quantitative Biology 2013-10-10 Namiko Mitarai , Steen Pedersen

We theoretically study the Totally Asymmetric Exclusion Process (TASEP) with quenched jumping rates disorder and finite lifetime chain. TASEP is widely used to model the translation of messenger RNAs by Ribosomes in protein synthesis. Since…

The ribosome flow model with input and output (RFMIO) is a deterministic dynamical system that has been used to study the flow of ribosomes during mRNA translation. The RFMIO and its variants encapsulate important properties that are…

Molecular Networks · Quantitative Biology 2018-11-20 Itzik Nanikashvili , Yoram Zarai , Alexander Ovseevich , Tamir Tuller , Michael Margaliot

The ribosome is a macromolecular complex which is responsible for protein synthesis in all living cells according to their transcribed genetic information. Using X-ray crystallography and, more recently, cryo-electron microscopy (cryo-EM),…

Biological Physics · Physics 2017-11-17 Lars V. Bock , Michal H. Kolář , Helmut Grubmüller

The cytoskeleton in eukaryotic cells plays several crucial roles. In terms of intracellular transport, motor proteins use the cytoskeletal filaments as a backbone along which they can actively transport biological cargos such as vesicles…

Biological Physics · Physics 2020-06-20 Adélaïde Raguin , Norbert Kern , Andrea Parmeggiani

Transcription is a complex phenomenon that permits the conversion of genetic information into phenotype by means of an enzyme called RNA polymerase, which erratically moves along and scans the DNA template. We perform Bayesian inference…

Biological Physics · Physics 2023-07-18 Massimo Cavallaro , Yuexuan Wang , Daniel Hebenstreit , Ritabrata Dutta