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We show that the modulation of the phases of the laser beams of ultra-short pulses leads to modulation of the two photon fluorescence intensity. The phase modulation technique when used in multi-photon microscopy can improve the signal to…

Optics · Physics 2015-03-12 Khadga Jung Karki , Mohammed Abdellah , Tonu Pullerits

A mixed precision Fast Fourier transform (FFT) implementation is presented. The procedure uses per-block microscaling (MX), a global power-of-two prescale, and prequantized low bit twiddles. We evaluate forward and round-trip FFT fidelity…

Signal Processing · Electrical Eng. & Systems 2026-02-18 Nikhil Deveshwar , Abhejit Rajagopal , Peder E. Z. Larson

Optical photothermal microscopy is a powerful, emerging method that overcomes the diffraction limit in infrared hyperspectral imaging by utilizing a visible probe laser beam to detect local temperature-induced modulation at the visible…

The use of Fourier methods in wave-front reconstruction can significantly reduce the computation time for large telescopes with a high number of degrees of freedom. However, Fourier algorithms for discrete data require a rectangular data…

Instrumentation and Methods for Astrophysics · Physics 2017-06-07 Charlotte Z. Bond , Carlos M. Correia , Jean-François Sauvage , Benoit Neichel , Thierry Fusco

We consider blind ptychography, an imaging technique which aims to reconstruct an object of interest from a set of its diffraction patterns, each obtained by a local illumination. As the distribution of the light within the illuminated…

Numerical Analysis · Mathematics 2023-06-16 Oleh Melnyk

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

Image stacks provide invaluable 3D information in various biological and pathological imaging applications. Fourier ptychographic microscopy (FPM) enables reconstructing high-resolution, wide field-of-view image stacks without z-stack…

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

We report the implementation of a fully on-chip, lensless microscopy technique termed optofluidic ptychography. This imaging modality complements the miniaturization provided by microfluidics and allows the integration of ptychographic…

Instrumentation and Detectors · Physics 2021-12-21 Pengming Song , Chengfei Guo , Shaowei Jiang , Tianbo Wang , Patrick Hu , Derek Hu , Zibang Zhang , Bin Feng , Guoan Zheng

Fourier ptychographic microscopy (FPM), characterized by high-throughput computational imaging, theoretically provides a cunning solution to the trade-off between spatial resolution and field of view (FOV), which has a promising prospect in…

Optics · Physics 2024-02-29 Shuhe Zhang , Aiye Wang , Jinghao Xu , Tianci Feng , Jinhua Zhou , An Pan

The plug-and-play priors (PnP) framework has been recently shown to achieve state-of-the-art results in regularized image reconstruction by leveraging a sophisticated denoiser within an iterative algorithm. In this paper, we propose a new…

Computer Vision and Pattern Recognition · Computer Science 2021-03-25 Yu Sun , Shiqi Xu , Yunzhe Li , Lei Tian , Brendt Wohlberg , Ulugbek S. Kamilov

Fourier ptychography (FP) is a promising computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. However, to date, the…

Optics · Physics 2018-10-17 An Pan , Yan Zhang , Kai Wen , Maosen Li , Meiling Zhou , Junwei Min , Ming Lei , Baoli Yao

Digital aberration measurement and removal play a prominent role in computational imaging platforms aimed at achieving simple and compact optical arrangements. A recent important class of such platforms is Fourier ptychography, which is…

Ptychography is a lensless imaging technique, which considers reconstruction from a set of far-field diffraction patterns obtained by illuminating small overlapping regions of the specimen. In many cases, a distribution of light inside the…

Numerical Analysis · Mathematics 2022-10-05 Frank Filbir , Oleh Melnyk

Ptychography is now integrated as a tool in mainstream microscopy allowing quantitative and high-resolution imaging capabilities over a wide field of view. However, its ultimate performance is inevitably limited by the available coherent…

Computational Physics · Physics 2024-06-12 Wenhui Xu , Shoucong Ning , Pengju Sheng , Huixiang Lin , Angus I Kirkland , Yong Peng , Fucai Zhang

Ptychography involves a sample being illuminated by a coherent, localised probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the probe or sample is shifted…

Numerical Analysis · Mathematics 2023-05-09 Mark Philip Roach

Ptychography is an emerging imaging technique that is able to provide wavelength-limited spatial resolution from specimen with extended lateral dimensions. As a scanning microscopy method, a typical two-dimensional image requires a number…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Distinguishing visually similar objects like forged/authentic bills and healthy/unhealthy plants is beyond the capabilities of even the most sophisticated classifiers. We propose the use of multiplexed illumination to extend the range of…

Computer Vision and Pattern Recognition · Computer Science 2021-02-24 Taihua Wang , Donald G. Dansereau

In this paper, we propose a novel method for image fusion with a high-resolution panchromatic image and a low-resolution multispectral image at the same geographical location. The fusion is formulated as a convex optimization problem which…

Computer Vision and Pattern Recognition · Computer Science 2015-10-28 Chen Chen , Yeqing Li , Wei Liu , Junzhou Huang
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