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In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption…

Optics · Physics 2017-05-31 Thomas Chaigne , Bastien Arnal , Sergey Vilov , Emmanuel Bossy , Ori Katz

This article presents a new optical measurement method employing a HSI (Hue, Saturation and Intensity) colour model to form trapezoidal structured patterns for morphology reconstruction of a measured object at a high speed. Profilometry on…

Optics · Physics 2015-05-19 L C Chen , X L Nguyen , Y S Shu

Molecular and atomic imaging required the development of electron and scanning probe microscopies to surpass the physical limits dictated by diffraction. Nano-infrared experiments and pico-cavity tip-enhanced Raman spectroscopy imaging…

Finite element methods typically require a high resolution to satisfactorily approximate micro and even macro patterns of an underlying physical model. This issue can be circumvented by appropriate multiscale strategies that are able to…

Numerical Analysis · Mathematics 2025-12-24 Zhi-Song Liu , Roland Maier , Andreas Rupp

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

There is currently no widely adopted standard for the optical characterisation of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of…

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

Biomedical applications requiring tissue diagnosis, activation, and treatment could be substantially leveraged by optical methods, owing to their unique feature set. However, their widespread application is severely limited by the strong…

Optics · Physics 2019-10-23 Maxim Cherkashin , Carsten Brenner , Georg Schmitz , Martin Hofmann

A nanopatterning scheme is presented by which the structure height can be controlled in the tens of nanometers range and the lateral resolution is a factor at least three times better than the point spread function of the writing beam. The…

Mesoscale and Nanoscale Physics · Physics 2010-01-14 D. Kunik , L. I. Pietrasanta , O. E. Martínez

Insight in the structure of nanoparticle assemblies up to a single particle level is key to understand the collective properties of these assemblies, which critically depend on the individual particle positions and orientations. However,…

Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using the time-domain fingerprints from uniform nanoparticles. However, the optical diffraction limit compromises the decoding throughput and…

Optics · Physics 2022-08-09 Baolei Liu , Jiayan Liao , Yiliao Song , Jie Lu , Jiajia Zhou , Fan Wang

We develop a new process of image plane self-calibration for interferometric imaging data. The process is based on Shape-Orientation-Size (SOS) conservation for the principal triangle in an image generated from the three fringes made from a…

Instrumentation and Methods for Astrophysics · Physics 2022-11-01 C. L. Carilli , B. Nikolic , N. Thyagarajan

A widely tested approach to overcoming the diffraction limit in microscopy without disturbing the sample relies on substituting widefield sample illumination with a structured light beam. This gives rise to confocal, image-scanning and…

Optics · Physics 2024-06-03 Alexander Duplinskiy , Jernej Frank , Kaden Bearne , A. I. Lvovsky

Traceability to the International System of Units (SI) is fundamental to measurement accuracy and reliability. In this study, we demonstrate subnanometer traceability of localization microscopy, establishing a metrological foundation for…

We re-examine real-time holography for all-optical structuring of light and optical computation using a contemporary material: a subwavelength-thick, spatially unstructured film of indium tin oxide (ITO). When excited by spatially…

Talbot length, the distance between two consecutive self-image planes along the propagation axis for a periodic diffraction object (grating) illuminated by a plane wave, depends on the period of the object and the wavelength of…

Optics · Physics 2024-08-21 Saumya J. Sarkar , M. Ebrahim-Zadeh , G. K. Samanta

Light scattering by tissue severely limits how deep beneath the surface one can image, and the spatial resolution one can obtain from these images. Diffuse optical tomography (DOT) is one of the most powerful techniques for imaging deep…

Image and Video Processing · Electrical Eng. & Systems 2021-05-31 Yongyi Zhao , Ankit Raghuram , Hyun K. Kim , Andreas H. Hielscher , Jacob T. Robinson , Ashok Veeraraghavan

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller
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