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Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

An ultrafast single-pixel optical 2D imaging system using a single multimode fiber (MF) is proposed. The MF acted as the all-optical random pattern generator. Light with different wavelengths pass through a single MF will generator…

In this article, we provide an alternative up-sampling and PSF deconvolution method for the iterative multi-exposure coaddition. Different from the previous works, the new method has a ratio-correction term, which allows the iterations to…

Instrumentation and Methods for Astrophysics · Physics 2022-10-26 Lei Wang , Guoliang Li , Xi Kang

One of the main characteristics of optical imaging systems is the spatial resolution, which is restricted by the diffraction limit to approximately half the wavelength of the incident light. Along with the recently developed classical…

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…

Instrumentation and Detectors · Physics 2023-05-10 Philipp Johannes Schubert , Rangoli Saxena , Joergen Kornfeld

On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and…

Motivated by the importance of optical microscopes to science and engineering, scientists have pondered for centuries how to improve their resolution and the existence of fundamental resolution limits. In recent years, a new class of…

Quantum Physics · Physics 2015-07-28 Mankei Tsang

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Second harmonic generation (SHG) microscopy is a valuable tool for optical microscopy. SHG microscopy is normally performed as a point scanning imaging method, which lacks phase information and is limited in spatial resolution by the…

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

This study explores the use of photometric techniques (shape-from-shading and uncalibrated photometric stereo) for upsampling the low-resolution depth map from an RGB-D sensor to the higher resolution of the companion RGB image. A…

Computer Vision and Pattern Recognition · Computer Science 2019-06-26 Bjoern Haefner , Songyou Peng , Alok Verma , Yvain Quéau , Daniel Cremers

In this paper, we present a new feature selection method that is suitable for both unsupervised and supervised problems. We build upon the recently proposed Infinite Feature Selection (IFS) method where feature subsets of all sizes…

Machine Learning · Computer Science 2017-08-22 Sadegh Eskandari , Emre Akbas

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…

Image and Video Processing · Electrical Eng. & Systems 2021-06-07 Figen S. Oktem , Oğuzhan Fatih Kar , Can Deniz Bezek , Farzad Kamalabadi

Conventional microscope objective lenses are diffraction limited, which means that they cannot resolve features smaller than half the illumination wavelength. Under white light illumination, such resolution limit is about 250-300 nm for an…

Instrumentation and Detectors · Physics 2021-08-26 Bing Yan , Zengbo Wang , Alan Parker , Yukun Lai , John Thomas , Liyang Yue , James Monks

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…

Medical Physics · Physics 2022-07-12 Vasiliki Stergiopoulou , Luca Calatroni , Sébastien Schaub , Laure Blanc-Féraud

A widely tested approach to overcoming the diffraction limit in microscopy without disturbing the sample relies on substituting widefield sample illumination with a structured light beam. This gives rise to confocal, image-scanning and…

Optics · Physics 2024-06-03 Alexander Duplinskiy , Jernej Frank , Kaden Bearne , A. I. Lvovsky

In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…

Optics · Physics 2021-01-07 Antoine Boniface , Jonathan Dong , Sylvain Gigan

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

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