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Optical diffraction tomography (ODT) is an interferometric microscopy technique capable of measuring 3-D refractive index (RI) distribution of transparent samples. Multiple 2-D holograms of a sample illuminated with various angles are…

Optical diffraction tomography (ODT) is a powerful non-invasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral…

Three-dimensional imaging of biological cells is crucial for the investigation of cell biology, provide valuable information to reveal the mechanisms behind pathophysiology of cells and tissues. Recent advances in optical diffraction…

Biological Physics · Physics 2016-03-03 Kyoohyun Kim , Jonghee Yoon , Seungwoo Shin , SangYun Lee , Su-A Yang , YongKeun Park

Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…

Optical diffraction tomography (ODT) is a three-dimensional (3D) quantitative phase imaging technique, which enables the reconstruction of the 3D refractive index (RI) distribution of a transparent sample. Due to its fast, non-invasive, and…

Optics · Physics 2018-11-14 Chansuk Park , Seungwoo Shin , Yongkeun Park

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

Optics · Physics 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI).…

Quantitative Methods · Quantitative Biology 2017-08-14 Mirjam Schürmann , Gheorghe Cojoc , Salvatore Girardo , Elke Ulbricht , Jochen Guck , Paul Müller

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

Optics · Physics 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric…

Image and Video Processing · Electrical Eng. & Systems 2024-12-13 Shwetadwip Chowdhury , Michael Chen , Regina Eckert , David Ren , Fan Wu , Nicole Repina , Laura Waller

Optical diffraction tomography (ODT), initially described in the seminal paper of Emil Wolf [Opt. Comm., 1(4), 153-156 (1969)], has received renewed attention recently. The vast majority of ODT experiments reported to date have been 3D…

Optical diffraction tomography (ODT) is an emerging 3D imaging technique that is used for the 3D reconstruction of the refractive index (RI) for semi-transparent samples. Various inverse models have been proposed to reconstruct the 3D RI…

Optics · Physics 2022-06-13 Ahmed B. Ayoub , Amirhossein Saba , Carlo Gigli , Demetri Psaltis

The measurement of three-dimensional (3D) images and the analysis of subcellular organelles are crucial for the study of the pathophysiology of cells and tissues. Optical diffraction tomography (ODT) facilitates label-free and quantitative…

Image and Video Processing · Electrical Eng. & Systems 2019-10-08 Taean Chang , Seungwoo Shin , Moosung Lee , YongKeun Park

Optical diffraction tomography (ODT) provides three-dimensional refractive index (RI) tomograms of a transparent microscopic object. However, because of the finite numerical aperture of objective lenses, ODT has the limited access to…

Optics · Physics 2017-11-22 Moosung Lee , Seungwoo Shin , YongKeun Park

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Herein is presented an optical diffraction tomography (ODT) technique for measuring 3-D refractive index (RI) maps of optical plastic lenses. A Mach-Zehnder interferometer was used to measure multiple complex optical fields of a plastic…

Optics · Physics 2016-04-20 Kyoohyun Kim , Jonghee Yoon , YongKeun Park

Optical diffraction tomography (ODT) is a powerful technique for quantitative, label-free reconstruction of the three-dimensional refractive index (RI) distribution of biological samples. While ODT is well established for imaging thin,…

Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high…

In optical diffraction tomography (ODT), the three-dimensional scattering potential of a microscopic object rotating around its center is recovered by a series of illuminations with coherent light. Reconstruction algorithms such as the…

Numerical Analysis · Mathematics 2022-09-02 Robert Beinert , Michael Quellmalz

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

Image and Video Processing · Electrical Eng. & Systems 2025-04-22 N Goyal , K Khare
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