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Orbit recovery is a central problem in both mathematics and applied sciences, with important applications to structural biology. This paper focuses on recovering generic orbits of functions on ${\mathbb R}^{n}$ and the sphere $S^{n-1}$…

Numerical Analysis · Mathematics 2025-08-06 Tamir Bendory , Dan Edidin , Josh Katz , Shay Kreymer

In cryo-electron microscopy (cryo-EM), a microscope generates a top view of a sample of randomly-oriented copies of a molecule. The problem of single particle reconstruction (SPR) from cryo-EM is to use the resulting set of noisy 2D…

Numerical Analysis · Mathematics 2014-09-16 Gene Katsevich , Alexander Katsevich , Amit Singer

Single-particle electron cryomicroscopy (cryo-EM) is an increasingly popular technique for elucidating the three-dimensional structure of proteins and other biologically significant complexes at near-atomic resolution. It is an imaging…

Computational Physics · Physics 2020-04-01 Amit Singer , Fred J. Sigworth

Semi-algebraic priors are ubiquitous in signal processing and machine learning. Prevalent examples include a) linear models where the signal lies in a low-dimensional subspace; b) sparse models where the signal can be represented by only a…

Information Theory · Computer Science 2025-08-19 Tamir Bendory , Nadav Dym , Dan Edidin , Arun Suresh

While most existing sparse recovery results allow only minimal structure within the measurement scheme, many practical problems possess significant structure. To address this gap, we present a framework for structured measurements that are…

Information Theory · Computer Science 2025-07-28 Timm Gilles , Hartmut Führ

Single particle cryogenic electron microscopy (cryo-EM) is an imaging technique capable of recovering the high-resolution 3-D structure of biological macromolecules from many noisy and randomly oriented projection images. One notable…

Biomolecules · Quantitative Biology 2024-01-30 Andy Zhang , Oscar Mickelin , Joe Kileel , Eric J. Verbeke , Nicholas F. Marshall , Marc Aurèle Gilles , Amit Singer

Motivated by the task of 2-D classification in single particle reconstruction by cryo-electron microscopy (cryo-EM), we consider the problem of heterogeneous multireference alignment of images. In this problem, the goal is to estimate a…

Image and Video Processing · Electrical Eng. & Systems 2019-10-03 Chao Ma , Tamir Bendory , Nicolas Boumal , Fred Sigworth , Amit Singer

This work studies the sample complexity of the multi-target detection (MTD) problem, which involves recovering a signal from a noisy measurement containing multiple instances of a target signal in unknown locations, each transformed by a…

Signal Processing · Electrical Eng. & Systems 2025-01-22 Amnon Balanov , Shay Kreymer , Tamir Bendory

Electron cryomicroscopy (cryo-EM) is a technique in structural biology used to reconstruct accurate volumetric maps of molecules. One step of the cryo-EM pipeline involves solving an inverse-problem. This inverse-problem, referred to as…

Quantitative Methods · Quantitative Biology 2024-11-26 Aaditya V. Rangan , Wai-Shing Tang , Pilar Cossio , Kexin Zhang , Nikolaus Grigorieff

When a group acts on a set, it naturally partitions it into orbits, giving rise to orbit problems. These are natural algorithmic problems, as symmetries are central in numerous questions and structures in physics, mathematics, computer…

Computational Complexity · Computer Science 2025-10-14 Peter Bürgisser , Mahmut Levent Doğan , Visu Makam , Michael Walter , Avi Wigderson

A major challenge in single-particle cryo-electron microscopy (cryo-EM) is that the orientations adopted by the 3D particles prior to imaging are unknown; yet, this knowledge is essential for high-resolution reconstruction. We present a…

Machine Learning · Computer Science 2021-04-14 Jelena Banjac , Laurène Donati , Michaël Defferrard

We propose a framework to jointly determine the deformation parameters and reconstruct the unknown volume in electron cryotomography (CryoET). CryoET aims to reconstruct three-dimensional biological samples from two-dimensional projections.…

Image and Video Processing · Electrical Eng. & Systems 2023-10-17 Valentin Debarnot , Sidharth Gupta , Konik Kothari , Ivan Dokmanic

Cryo-EM is a vital technique for determining 3D structure of biological molecules such as proteins and viruses. The cryo-EM reconstruction problem is challenging due to the high noise levels, the missing poses of particles, and the…

Quantitative Methods · Quantitative Biology 2024-06-05 Larissa de Ruijter , Gabriele Cesa

Isometries are ubiquitous in nature; isometries of discrete (quantized) objects---abstracted as the group of isometries of $\mathbb{Z}^n$ denoted by $\mathsf{ISO}(\mathbb{Z}^n)$---are important concepts in the computational world. In this…

Group Theory · Mathematics 2020-03-13 Haizi Yu , Igor Mineyev , Lav R. Varshney

Cryo-electron microscopy (cryo-EM) has recently become a premier method for obtaining high-resolution structures of biological macromolecules. However, it is limited to biomolecular samples with low conformational heterogeneity, where all…

In physical systems possessing symmetry, reconstructing the underlying causal structure from observational data constitutes an inverse problem of fundamental importance. In this work, we formulate the inverse problem of causal inference…

Mathematical Physics · Physics 2025-11-13 Isshin Arai , Tomoaki Itano

The reconstruction of an unknown quantity from noisy measurements is a mathematical problem relevant in most applied sciences, for example, in medical imaging, radar inverse scattering, or astronomy. This underlying mathematical problem is…

Optimization and Control · Mathematics 2025-10-14 Nina M. Gottschling , David Iagaru , Jakob Gawlikowski , Ioannis Sgouralis

Cryo-electron microscopy (cryo-EM) is capable of producing reconstructed 3D images of biomolecules at near-atomic resolution. As such, it represents one of the most promising imaging techniques in structural biology. However, raw cryo-EM…

Quantitative Methods · Quantitative Biology 2021-05-25 Nina Miolane , Frédéric Poitevin , Yee-Ting Li , Susan Holmes

The computational pipelines of single-particle cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) include an early particle-picking stage, in which a micrograph or tomogram is scanned to extract candidate particles,…

Signal Processing · Electrical Eng. & Systems 2026-04-14 Amnon Balanov , Alon Zabatani , Tamir Bendory

A single-particle cryo-electron microscopy (cryo-EM) measurement, called a micrograph, consists of multiple two-dimensional tomographic projections of a three-dimensional (3-D) molecular structure at unknown locations, taken under unknown…

Image and Video Processing · Electrical Eng. & Systems 2026-03-10 Shay Kreymer , Amit Singer , Tamir Bendory