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Nonlinear optical microscopy allows rapid high-resolution microscopy with image contrast generated from intrinsic properties of the sample. Established modalities such as multiphoton excited fluorescence and second/third-harmonic generation…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Coded Aperture Snapshot Spectral Imaging (CASSI) reconstruction aims to recover the 3D spatial-spectral signal from 2D measurement. Existing methods for reconstructing Hyperspectral Image (HSI) typically involve learning mappings from a 2D…

Image and Video Processing · Electrical Eng. & Systems 2025-03-19 Huan Chen , Wangcai Zhao , Tingfa Xu , Shiyun Zhou , Peifu Liu , Jianan Li

Natural signals and images are well-known to be approximately sparse in transform domains such as Wavelets and DCT. This property has been heavily exploited in various applications in image processing and medical imaging. Compressed sensing…

Machine Learning · Computer Science 2015-10-26 Saiprasad Ravishankar , Yoram Bresler

We report a novel extension to structured illumination (SI) microscopy that utilizes the Talbot self-imaging effect to generate a SI pattern on a sample with field-of-view (FOV) and resolution unconstrained by the numerical aperture (NA) of…

Optics · Physics 2018-01-12 Shwetadwip Chowdhury , Jeffrey Chen , Joseph A. Izatt

In recent years several methods to overcome diffraction limit in the far field microscopy have been demonstrated. Still the problem of superresolution is reliably solved only for fluorescent microscopy, giving a resolution of up to 20-30nm.…

Optics · Physics 2013-09-03 Yu. V. Miklyaev , S. A. Asselborn , K. A. Zaytsev , M. Ya. Darscht

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Volumetric biological imaging often involves compromising high temporal resolution at the expense of high spatial resolution when popular scanning methods are used to capture 3D information. We introduce an integrated experimental and image…

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

Super-resolution effects in optical and fluorescence biological systems are analyzed and their relations with uncertainty relations are discussed. Super-resolutions obtained in the optical systems, including especially NSOM, SIM and…

Optics · Physics 2015-06-12 Yacob Ben-Aryeh

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as…

Instrumentation and Detectors · Physics 2018-12-19 Shiming Hu , Lei Liu , Yizheng Huang , Wenwen Liu , Qingquan Wei , Manqing Tan , Yude Yu

We introduce Linearly Constrained Diffusion Implicit Models (CDIM), a fast and accurate approach to solving noisy linear inverse problems using diffusion models. Traditional diffusion-based inverse methods rely on numerous projection steps…

Machine Learning · Computer Science 2025-12-01 Vivek Jayaram , Ira Kemelmacher-Shlizerman , Steven M. Seitz , John Thickstun

With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the…

Optics · Physics 2012-05-29 Chris J. Lee , Klaus J. Boller

Volumetric optical microscopy using non-diffracting beams enables rapid imaging of 3D volumes by projecting them axially to 2D images but lacks crucial depth information. Addressing this, we introduce MicroDiffusion, a pioneering tool…

Image and Video Processing · Electrical Eng. & Systems 2024-03-19 Mude Hui , Zihao Wei , Hongru Zhu , Fei Xia , Yuyin Zhou

Recently, chiral structured illumination microscopy has been proposed to image fluorescent chiral domains at sub-wavelength resolution. Chiral structured illumination microscopy is based on the combination of structured illumination…

Optics · Physics 2021-07-28 Shiang-Yu Huang , Ankit Kumar Singh , Jer-Shing Huang

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…