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This paper presents a polarization calibration method applied to a microwave polarimeter demonstrator based on a near-infrared (NIR) frequency up-conversion stage that allows both optical correlation and signal detection at a wavelength of…

Instrumentation and Methods for Astrophysics · Physics 2022-10-25 Francisco J. Casas , Patricio Vielva , R. Belen Barreiro , Enrique Martínez-González , G. Pascual-Cisneros

Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…

Applied Physics · Physics 2018-11-21 Miao-Hsuan Chien , Mario Brameshuber , Gerhard J. Schütz , Silvan Schmid

Diffuse direct time-of-flight LiDARs report per-pixel depth histograms formed by aggregating photon returns over a wide instantaneous field of view, violating the single-ray assumption behind standard LiDAR-RGB calibration. We present a…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Nikhil Behari , Ramesh Raskar

Single nanoparticle tracking using optical microscopy is a powerful technique with many applications in biology, chemistry and material sciences. Despite significant advances, localising objects with nanometric position accuracy in a…

Purpose: Handheld gamma cameras with coded aperture collimators are under investigation for intraoperative imaging in nuclear medicine. Coded apertures are a promising collimation technique for applications such as lymph node localization…

Medical Physics · Physics 2024-01-22 Tobias Meißner , Laura Antonia Cerbone , Paolo Russo , Werner Nahm , Jürgen Hesser

The single sideband (SSB) framework of analytical electron ptychography can account for the presence of residual geometrical aberrations induced by the probe-forming lens. However, the accuracy of this aberration correction method is highly…

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Image and Video Processing · Electrical Eng. & Systems 2024-03-07 Olivier Leblanc , Mathias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

Single particle reconstruction has recently emerged in 3D fluorescence microscopy as a powerful technique to improve the axial resolution and the degree of fluorescent labeling. It is based on the reconstruction of an average volume of a…

Image and Video Processing · Electrical Eng. & Systems 2023-01-24 Thibaut Eloy , Etienne Baudrier , Marine Laporte , Virginie Hamel , Paul Guichard , Denis Fortun

The accurate determination of atom numbers is an ubiquitous problem in the field of ultracold atoms. For modest atom numbers, absolute calibration techniques are available, however, for large numbers and high densities, the available…

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

We demonstrate that a common-line method can assemble a 3D oversampled diffracted intensity distribution suitable for high-resolution structure solution from a set of measured 2D diffraction patterns, as proposed in experiments with an…

Biomolecules · Quantitative Biology 2007-10-16 V. L. Shneerson , A. Ourmazd , D. K. Saldin

Achieving atomic resolution in electron microscopy has historically been hindered by spherical aberration, a fundamental limitation of conventional electron lenses. Its correction typically requires complex assemblies of electromagnetic…

The rich information content of measurements in the molecular frame rather than the laboratory frame has motivated the development of several methods for aligning gas phase molecules in space. Even so, for asymmetric tops the problem of…

Atomic Physics · Physics 2016-11-23 Varun Makhija , Xiaoming Ren , Drue Gockel , Anh-Thu Le , Vinod Kumarappan

We present monostatic sampling methods for limited-aperture scattering problems in two dimensions. The direct sampling method (DSM) is well known to provide a robust, stable, and fast numerical scheme for imaging inhomogeneities from…

Numerical Analysis · Mathematics 2022-04-14 Sangwoo Kang , Mikyoung Lim

Simultaneous, diffraction-limited control of multiple optical beams is crucial for applications ranging from lithography to optogenetics, deep tissue imaging, and tweezer-based manipulation of cells, particles, or atoms. Despite the desire…

Optics · Physics 2025-05-14 Ian Christen , Christopher Panuski , Thomas Propson , Dirk Englund

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

Mesoscale and Nanoscale Physics · Physics 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

In diffusion MRI (dMRI), a good sampling scheme is important for efficient acquisition and robust reconstruction. Diffusion weighted signal is normally acquired on single or multiple shells in q-space. Signal samples are typically…

Medical Physics · Physics 2017-09-26 Jian Cheng , Dinggang Shen , Pew-Thian Yap , Peter J. Basser

Important applications of single-particle tracking (SPT) aim at deciphering the diffusion properties of single fluorescent nanoparticles immersed in heterogeneous environments, such as multi-cellular biological tissues. To maximize the…

Fixing molecules in space is a crucial step for the imaging of molecular structure and dynamics. Here, we demonstrate three-dimensional (3D) field-free alignment of the prototypical asymmetric top molecule indole using elliptically…