Related papers: Aberration-free calibration for 3D single molecule…
Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…
Accurate source localization in Multi-Platform Radar Networks (MPRNs) benefits from exploiting both range and angle measurements under robust estimation. In this paper, we propose a robust Euclidean distance matrix (EDM) optimization model…
In the study of nanobubbles, nanodroplets or nanolenses immobilised on a substrate, a cross-section of a spherical-cap is widely applied to extract geometrical information from atomic force microscopy (AFM) topographic images. In this…
The key to accurate 3D shape measurement in Fringe Projection Profilometry (FPP) is the proper calibration of the measurement system. Current calibration techniques rely on phase-coordinate mapping (PCM) or back-projection stereo-vision…
Correlative light electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe.…
A method of distributed calibration of a probe microscope scanner is suggested which main idea consists in a search for a net of local calibration coefficients (LCCs) in the process of automatic measurement of a standard surface, whereby…
The usual method for determining the sensitivity curve of a TXRF spectrometer relies on calibration using a set of vendor-certified concentration values of reference calibration standards. These samples, which are certified by the provider,…
We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…
Precise manipulation of single molecules has already led to remarkable insights in physics, chemistry, biology and medicine. However, widespread adoption of single-molecule techniques has been impeded by equipment cost and the laborious…
Traditional weak gravitational lensing shear estimators are carefully calibrated but struggle to fully capture realistic galaxy morphologies, point-spread-function (PSF) effects, blending, and noise in deep surveys, while blindly trained…
We present an ultra-fast, precise, parameter-free method, which we term Deep-STORM, for obtaining super-resolution images from stochastically-blinking emitters, such as fluorescent molecules used for localization microscopy. Deep-STORM uses…
Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…
Statistical Shape Modeling (SSM) is a quantitative method for analyzing morphological variations in anatomical structures. These analyses often necessitate building models on targeted anatomical regions of interest to focus on specific…
We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based $\ell_0$…
Single-molecule localization microscopy (SMLM) surpasses the diffraction limit, achieving subcellular resolution. Traditional SMLM analysis methods often rely on point spread function (PSF) model fitting, limiting the application of complex…
Camera calibration is a process of paramount importance in computer vision applications that require accurate quantitative measurements. The popular method developed by Zhang relies on the use of a large number of images of a planar grid of…
Recent progress has boosted the resolving power of optical microscopies to spatial dimensions well below the diffraction limit. Yet, axial super-resolution and axial single-molecule localisation typically require more complicated…
This study is concerned with the design of a Mueller imaging polarimeter for the visualization of spatially-varying Mueller matrix fields. A simplified calibration procedure is advocated, where all the optical elements are calibrated…
Spatially varying spectral modulation can be implemented using a liquid crystal spatial light modulator (SLM) since it provides an array of liquid crystal cells, each of which can be purposed to act as a programmable spectral filter array.…
The lensless endoscope (LE) is a promising device to acquire in vivo images at a cellular scale. The tiny size of the probe enables a deep exploration of the tissues. Lensless endoscopy with a multicore fiber (MCF) commonly uses a spatial…