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Solvation is essential for protein activities. To study internal solvation of protein, site-directed mutagenesis is applied. Intrinsic fluorescent probe, tryptophan, is inserted into desired position inside protein molecule for ultrafast…

Biological Physics · Physics 2015-10-07 Gao Guang-yu , Li Yu , Wang Wei , Wang Shu-feng , Dongping Zhong , Gong Qi-huang

Discerning how a mutation affects the stability of a protein is central to the study of a wide range of diseases. Machine learning and statistical analysis techniques can inform how to allocate limited resources to the considerable time and…

Quantitative Methods · Quantitative Biology 2018-03-14 Richard Olney , Aaron Tuor , Filip Jagodzinski , Brian Hutchinson

Genome rearrangements are events where large blocks of DNA exchange places during evolution. The analysis of these events is a promising tool for understanding evolutionary genomics, providing data for phylogenetic reconstruction based on…

Computational Complexity · Computer Science 2023-11-30 Luís Cunha , Ignasi Sau , Uéverton Souza

Technical signs of progress during the last decades has led to a situation in which the accumulation of genome sequence data is increasingly fast and cheap. The huge amount of molecular data available nowadays can help addressing new and…

Genomics · Quantitative Biology 2017-05-02 Christophe Guyeux , Bashar Al-Nuaimi , Bassam AlKindy , Jean-François Couchot , Michel Salomon

DNA replication is an essential process in biology and its timing must be robust so that cells can divide properly. Random fluctuations in the formation of replication starting points, called origins, and the subsequent activation of…

Quantitative Methods · Quantitative Biology 2012-02-03 Jens Karschau , J. Julian Blow , Alessandro P. S. de Moura

Modellers of large scale genome rearrangement events, in which segments of DNA are inverted, moved, swapped, or even inserted or deleted, have found a natural syntax in the language of permutations. Despite this, there has been a wide range…

Other Quantitative Biology · Quantitative Biology 2016-10-04 Sangeeta Bhatia , Pedro Feijão , Andrew R. Francis

How DNA-binding proteins locate specific genomic targets remains a central challenge in molecular biology. Traditional protein-centric approaches, which rely on wet-lab experiments and visualization techniques, often lack genome-wide…

Quantitative Methods · Quantitative Biology 2025-09-16 Li Yang , Dongbo Wang

A common approach to quantifying DNA involves repeated cycles of DNA amplification. This approach, employed by the polymerase chain reaction (PCR), produces outputs that are corrupted by amplification noise, making it challenging to…

Quantitative Methods · Quantitative Biology 2023-01-06 Abdoelnaser M Degoot , Wilfred Ndifon

Statistical analysis of DNA mixtures is known to pose computational challenges due to the enormous state space of possible DNA profiles. We propose a Bayesian network representation for genotypes, allowing computations to be performed…

Methodology · Statistics 2014-02-21 Therese Graversen , Steffen Lauritzen

The site frequency spectrum describes variation among a set of n DNA sequences. Its i'th entry (i=1,2,...,n-1) is the number of nucleotide sites at which the mutant allele is present in i copies. Under selective neutrality, random mating,…

Populations and Evolution · Quantitative Biology 2021-03-02 Alan R. Rogers , Stephen P. Wooding

The efficient recognition of pathogens by the adaptive immune system relies on the diversity of receptors displayed at the surface of immune cells. T-cell receptor diversity results from an initial random DNA editing process, called VDJ…

Populations and Evolution · Quantitative Biology 2015-04-28 Yuval Elhanati , Anand Murugan , Curtis G. Callan , Thierry Mora , Aleksandra M. Walczak

Protein-protein interaction (PPI) represents a central challenge within the biology field, and accurately predicting the consequences of mutations in this context is crucial for drug design and protein engineering. Deep learning (DL) has…

Machine Learning · Computer Science 2026-01-13 Fang Wu , Stan Z. Li

Systematic characterization of biological effects to genetic perturbation is essential to the application of molecular biology and biomedicine. However, the experimental exhaustion of genetic perturbations on the genome-wide scale is…

Genomics · Quantitative Biology 2024-03-06 Lingmin Zhan , Yuanyuan Zhang , Yingdong Wang , Aoyi Wang , Caiping Cheng , Jinzhong Zhao , Wuxia Zhang , Peng Lia , Jianxin Chen

We report on a theoretical study of point mutations effects on charge transfer properties in the DNA sequence of the tumor-suppressor p53 gene. On the basis of effective single-strand or double-strand tight-binding models which simulate…

Genomics · Quantitative Biology 2008-01-09 Chi-Tin Shih , Stephan Roche , Rudolf A. Römer

Genome rearrangements are evolutionary events that shuffle genomic architectures. Most frequent genome rearrangements are reversals, translocations, fusions, and fissions. While there are some more complex genome rearrangements such as…

Genomics · Quantitative Biology 2015-04-07 Nikita Alexeev , Rustem Aidagulov , Max A. Alekseyev

DNA methylation plays a pivotal role in the genetic evolution of both embryonic and adult cells. For adult somatic cells, location and dynamics of methylation has been very precisely pinned down with the 5-cytosine markers on…

Mesoscale and Nanoscale Physics · Physics 2014-06-04 Towfiq Ahmed , Jason T. Haraldsen , Jian-Xin Zhu , Alexander V. Balatsky

Stochastic rearrangement of germline DNA by VDJ recombination is at the origin of immune system diversity. This process is implemented via a series of stochastic molecular events involving gene choices and random nucleotide insertions…

Quantitative Methods · Quantitative Biology 2012-11-12 Anand Murugan , Thierry Mora , Aleksandra M. Walczak , Curtis G. Callan

We present experimental results regarding the electrotransfer of plasmid DNA into phosphatidylcholine giant unilamellar vesicles (GUVs). Our observations indicate that a direct entry is the predominant mechanism of electrotransfer. A…

Biological Physics · Physics 2011-02-07 Thomas Portet , Cyril Favard , Justin Teissié , David S. Dean , Marie-Pierre Rols

Background: Significance analysis plays a major role in identifying and ranking genes, transcription factor binding sites, DNA methylation regions, and other high-throughput features for association with disease. We propose a new approach,…

Methodology · Statistics 2017-01-10 Andrew E. Jaffe , John D. Storey , Hongkai Ji , Jeffrey T. Leek

A common problem in bioinformatics is related to identifying gene regulatory regions marked by relatively high frequencies of motifs, or deoxyribonucleic acid sequences that often code for transcription and enhancer proteins. Predicting…

Genomics · Quantitative Biology 2021-01-22 Ethan Jacob Moyer , Anup Das