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Understanding the relationship between spontaneous stochastic fluctuations and the topology of the underlying gene regulatory network is of fundamental importance for the study of single-cell stochastic gene expression. Here by solving the…

Molecular Networks · Quantitative Biology 2017-10-25 Chen Jia , Peng Xie , Min Chen , Michael Q. Zhang

We study the fluctuations of systems modeled by Markov jump processes with periodic generators. We focus on observables defined through time-periodic functions of the system's states or transitions. Using large deviation theory, canonical…

Statistical Mechanics · Physics 2020-04-22 Lydia Chabane , Raphaël Chétrite , Gatien Verley

Dynamic heterogeneity has often been modeled by assuming that a single-particle observable, fluctuating at a molecular scale, is influenced by its coupling to environmental variables fluctuating on a second, perhaps slower, time scale.…

Condensed Matter · Physics 2009-11-07 Gregor Diezemann , Gerald Hinze , Hans Sillescu

Gene expression in individual cells is highly variable and sporadic, often resulting in the synthesis of mRNAs and proteins in bursts. Bursting in gene expression is known to impact cell-fate in diverse systems ranging from latency in HIV-1…

Molecular Networks · Quantitative Biology 2016-02-17 Niraj Kumar , Abhyudai Singh , Rahul V. Kulkarni

Observation of phenotypic diversity in a population of genetically identical cells is often linked to the stochastic nature of chemical reactions involved in gene regulatory networks. We investigate the distribution of population averaged…

Molecular Networks · Quantitative Biology 2015-06-18 Bhaswati Bhattacharyya , Ziya Kalay

Recent experiments at the level of a single cell have shown that gene expression occurs in abrupt stochastic bursts. Further, in an ensemble of cells, the levels of proteins produced have a bimodal distribution. In a large fraction of…

Soft Condensed Matter · Physics 2009-11-07 Siddhartha Roy , Indrani Bose , Subhrangshu Sekhar Manna

Multiple phenotypic states often arise in a single cell with different gene-expression states that undergo transcription regulation with positive feedback. Recent experiments have shown that at least in E. coli, the gene state switching can…

Molecular Networks · Quantitative Biology 2015-10-28 Hao Ge , Hong Qian , Sunney Xiaoliang Xie

Gene expression is a stochastic process governed by the presence of specific transcription factors. Here we study the dynamics of gene expression in the presence of feedback, where a gene regulates its own expression. The nonlinear coupling…

Cell Behavior · Quantitative Biology 2008-07-23 Otto Pulkkinen , Johannes Berg

Auto-regulatory feedback loops are one of the most common network motifs. A wide variety of stochastic models have been constructed to understand how the fluctuations in protein numbers in these loops are influenced by the kinetic…

Subcellular Processes · Quantitative Biology 2020-04-22 James Holehouse , Zhixing Cao , Ramon Grima

Gene expression has a stochastic component owing to the single molecule nature of the gene and the small number of copies of individual DNA binding proteins in the cell. We show how the statistics of such systems can be mapped on to quantum…

Disordered Systems and Neural Networks · Physics 2009-11-10 Masaki Sasai , Peter G. Wolynes

The recent development of single-cell transcriptomics has enabled gene expression to be measured in individual cells instead of being population-averaged. Despite this considerable precision improvement, inferring regulatory networks…

Molecular Networks · Quantitative Biology 2017-11-28 Ulysse Herbach , Arnaud Bonnaffoux , Thibault Espinasse , Olivier Gandrillon

Stochastic simulation can make the molecular processes of cellular control more vivid than the traditional differential-equation approach by generating typical system histories instead of just statistical measures such as the mean and…

Subcellular Processes · Quantitative Biology 2018-09-18 Kevin Y. Chen , Daniel M. Zuckerman , Philip C. Nelson

In this paper, we consider two stochastic models of gene expression in prokaryotic cells. In the first model, sixteen biochemical reactions involved in transcription, translation and transcriptional regulation in the presence of inducer…

Condensed Matter · Physics 2007-05-23 Indrani Bose , Rajesh Karmakar , Siddhartha Roy

The expression of genes usually follows a two-step procedure. First, a gene (encoded in the genome) is transcribed resulting in a strand of (messenger) RNA. Afterwards, the RNA is translated into protein. Classically, this gene expression…

Molecular Networks · Quantitative Biology 2014-08-08 Martin Jansen , Peter Pfaffelhuber

A key goal of systems biology is the predictive mathematical description of gene regulatory circuits. Different approaches are used such as deterministic and stochastic models, models that describe cell growth and division explicitly or…

Molecular Networks · Quantitative Biology 2012-10-12 Rahul Marathe , Veronika Bierbaum , David Gomez , Stefan Klumpp

Many fundamental cellular processes involve small numbers of molecules. When numbers are small, fluctuations dominate, and stochastic models, which account for these fluctuations, are required. In this chapter, we describe minimal…

Molecular Networks · Quantitative Biology 2015-10-05 Andrew Mugler , Sean Fancher

We study a stochastic model proposed recently in the genetic literature to explain the heterogeneity of cell populations or of gene products. Cells are located in two colonies, whose sizes fluctuate as birth and migration processes in…

Populations and Evolution · Quantitative Biology 2007-05-23 Martin Gander , Christian Mazza , Hansklaus Rummler

Cellular processes do not follow deterministic rules; even in identical environments genetically identical cells can make random choices leading to different phenotypes. This randomness originates from fluctuations present in the…

Molecular Networks · Quantitative Biology 2016-02-02 Elijah Roberts , Shay Be'er , Chris Bohrer , Rati Sharma , Michael Assaf

The effect of stochasticity, in the form of Gaussian white noise, in a predator-prey model with two distinct time-scales is presented. A supercritical singular Hopf bifurcation yields a Type II excitability in the deterministic model. We…

Dynamical Systems · Mathematics 2017-07-20 Susmita Sadhu

MicroRNA-mediated regulation of gene expression is characterised by some distinctive features that set it apart from unregulated and transcription factor-regulated gene expression. Recently, a mathematical model has been proposed to…

Quantitative Methods · Quantitative Biology 2012-10-19 Indrani Bose , Sayantari Ghosh