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This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront…

Background: Tumour budding is an independent predictor of metastasis and prognosis in colorectal cancer and is a vital part of the pathology specification report. In a conventional pathological section observation process, pathologists have…

Medical Physics · Physics 2025-03-21 Yiyan Su , Ruiqing Sun , Yajing Wang , Yanfeng xi , Shaohui Zhang

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

The knowledge of the exact structure of the optical system PSF enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications.…

We collaborated with Amgen and spent five years in designing and fabricating next generation multi-well plate imagers based on Fourier ptychographic microscopy (FPM). A 6-well imager (Emsight) and a low-cost parallel microscopic system (96…

Biological Physics · Physics 2020-01-01 An Pan , Antony C. S. Chan , Baoli Yao , Changhuei Yang

We report a novel generalized optical measurement system and computational approach to determine and correct aberrations in optical systems. We developed a computational imaging method capable of reconstructing an optical system's…

Image and Video Processing · Electrical Eng. & Systems 2019-01-10 Jaebum Chung , Gloria W. Martinez , Karen Lencioni , Srinivas Sadda , Changhuei Yang

Image stacks provide invaluable 3D information in various biological and pathological imaging applications. Fourier ptychographic microscopy (FPM) enables reconstructing high-resolution, wide field-of-view image stacks without z-stack…

Fast Fourier Transform based phase screen simulations give accurate results only when the screen size ($G$) is much larger than the outer scale parameter ($L_0$). Otherwise, they fall short in correctly predicting both the low and high…

Instrumentation and Methods for Astrophysics · Physics 2021-06-03 Sorabh Chhabra , Jyotirmay Paul , Anamparambu N. Ramaprakash , Avinash Surendran

The characterization of a quantum device is a crucial step in the development of quantum experiments. This is accomplished via Quantum Process Tomography, which combines the outcomes of different projective measurements to deliver a…

Quantum Physics · Physics 2025-06-26 Francesco Di Colandrea , Nazanin Dehghan , Alessio D'Errico , Ebrahim Karimi

We investigate methods to calibrate the non-common path aberrations at an adaptive optics system having a wavefront-correcting device working at an extremely high resolution (larger than 150x150). We use focal-plane images collected…

Instrumentation and Methods for Astrophysics · Physics 2015-06-22 Visa Korkiakoski , Christoph U. Keller , Niek Doelman , Matthew Kenworthy , Gilles Otten , Michel Verhaegen

Fourier ptychography (FP) is a powerful light-based synthetic aperture imaging technique that allows one to reconstruct a high-resolution, wide field-of-view image by computationally integrating a diverse collection of low-resolution,…

Image and Video Processing · Electrical Eng. & Systems 2025-07-21 Matthew A. Chan , Casey J. Pellizzari , Christopher A. Metzler

This paper presents a novel calibration algorithm for Multi-Focus Plenoptic Cameras (MFPCs) using raw images only. The design of such cameras is usually complex and relies on precise placement of optic elements. Several calibration…

Image and Video Processing · Electrical Eng. & Systems 2021-11-10 Mathieu Labussière , Céline Teulière , Frédéric Bernardin , Omar Ait-Aider

Convolutional neural networks (CNNs) have gained tremendous success in solving complex inverse problems. The aim of this work is to develop a novel CNN framework to reconstruct video sequence of dynamic live cells captured using a…

Computer Vision and Pattern Recognition · Computer Science 2020-10-29 Thanh Nguyen , Yujia Xue , Yunzhe Li , Lei Tian , George Nehmetallah

The computational imaging technique of Fourier Ptychographic Microscopy (FPM) enables high-resolution imaging with a wide field of view and can serve as an extremely valuable tool, e.g. in the classification of cells in medical…

Image and Video Processing · Electrical Eng. & Systems 2025-08-25 Navya Sonal Agarwal , Jan Philipp Schneider , Kanchana Vaishnavi Gandikota , Syed Muhammad Kazim , John Meshreki , Ivo Ihrke , Michael Moeller

In this work we propose an efficient and accurate multi-scale optical simulation algorithm by applying a numerical version of slowly varying envelope approximation in FEM. Specifically, we employ the fast iterative method to quickly compute…

Optics · Physics 2024-12-03 Fan Xiao , Jingwei Wang , Zhongfei Xiong , Yuntian Chen

Single-pixel imaging, with the advantages of a wide spectrum, beyond-visual-field imaging, and robustness to light scattering, has attracted increasing attention in recent years. Fourier single-pixel imaging (FSI) can reconstruct sharp…

Image and Video Processing · Electrical Eng. & Systems 2021-08-05 Dong Zhou , Jie Cao , Huan Cui , Qun Hao , Bing-Kun Chen , Kai Lin

This paper presents a new approach on stretch processing for a fine range estimation using MPM (Matrix Pencil Method). The conventional method utilizes FFT (Fast Fourier Transform) with limited range resolution with its fixed number of…

Computational Physics · Physics 2012-09-12 Minwook Kwon , Zhou Du , Jinwook Kim , Mingyu Yoon , Jinhwan Koh

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source…

We propose a numerical interferometry method for identification of optical multiply-scattering systems when only intensity can be measured. Our method simplifies the calibration of optical transmission matrices from a quadratic to a linear…

Signal Processing · Electrical Eng. & Systems 2020-02-17 Sidharth Gupta , Rémi Gribonval , Laurent Daudet , Ivan Dokmanić

Ptychography is a popular imaging technique that combines diffractive imaging with scanning microscopy. The technique consists of a coherent beam that is scanned across an object in a series of overlapping positions, leading to reliable and…

Numerical Analysis · Mathematics 2024-08-08 Ricardo Parada , Samy Wu Fung , Stanley Osher