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X-ray phase-contrast imaging has the potential to improve image contrast with lower dose by probing an object's refractive properties as well as its absorptive properties. To reconstruct a phase-contrast image from a raw dataset, a phase…

Medical Physics · Physics 2025-08-19 Giavanna Jadick , Patrick La Rivière

X-ray micro tomography is a non-destructive, three-dimensional inspection technique applied across a vast range of fields and disciplines, ranging from research to industrial, encompassing engineering, biology and medical research.…

X-ray ptychography has revolutionised nanoscale phase contrast imaging at large-scale synchrotron sources in recent years. We present here the first successful demonstration of the technique in a small-scale laboratory setting. We conducted…

Three-dimensional phase contrast imaging of multiply-scattering samples in X-ray and electron microscopy is extremely challenging, due to small numerical apertures, the unavailability of wavefront shaping optics, and the highly nonlinear…

Computational Physics · Physics 2021-06-02 Philipp M Pelz , Hamish G Brown , Jim Ciston , Scott D Findlay , Yaqian Zhang , Mary Scott , Colin Ophus

We outline some basics of imaging using both fully-coherent and partially-coherent X-ray beams, with an emphasis on phase-contrast imaging. We open with some of the basic notions of X-ray imaging, including the vacuum wave equations and the…

Image and Video Processing · Electrical Eng. & Systems 2021-11-16 David M. Paganin , Daniele Pelliccia

These tutorials introduce some basics of imaging with coherent X-rays, focusing on phase contrast. We consider the transport-of-intensity equation, as one particular method for X-ray phase contrast imaging among many, before passing on to…

Image and Video Processing · Electrical Eng. & Systems 2019-02-28 David M. Paganin , Daniele Pelliccia

New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…

Numerical Analysis · Mathematics 2018-03-28 Victor Palamodov

Analyser-Based Phase-Contrast imaging (ABPCI) is a phase-contrast imaging method that produces high contrast images of weakly absorbing materials. However, it is only sensitive to phase variations lying in the diffraction plane of the…

Medical Physics · Physics 2021-02-11 M. C. Chalmers , M. J. Kitchen , K. Uesugi , G. Falzon , P. Quin , K. M. Pavlov

Detection of phase variations across optically transparent samples is often a difficult task. We propose and demonstrate a compact, lightweight and low cost quantitative phase contrast imager. Light diffracted from a pinhole is incident on…

X-ray microtomography is a versatile tool allowing the measurement of the 3D structure of optically thick samples. As a non-destructive technique, it is readily adapted to 4D imaging, where a sample can be monitored over time, and…

Instrumentation and Detectors · Physics 2025-12-17 Harry Allan , Tom Partridge , Joseph Jacob , Marco Endrizzi

Electron tomography is a technique used in both materials science and structural biology to image features well below optical resolution limit. In this work, we present a new algorithm for reconstructing the three-dimensional(3D)…

Signal Processing · Electrical Eng. & Systems 2018-07-12 David Ren , Michael Chen , Laura Waller , Colin Ophus

X-ray phase-contrast imaging has recently led to a revolution in resolving power and tissue contrast in biomedical imaging, microscopy and materials science. The necessary high spatial coherence is currently provided by either large-scale…

Plasma Physics · Physics 2019-06-26 J. Wenz , S. Schleede , K. Khrennikov , M. Bech , P. Thibault , M. Heigoldt , F. Pfeiffer , S. Karsch

Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…

Analysis of PDEs · Mathematics 2026-05-29 Thorsten Hohage , Milad Karimi , Björn Müller

X-ray microtomography is a powerful non-destructive technique allowing 3D virtual histology of resected human tissue. The achievable imaging field-of-view, is however limited by the fixed number of detector elements, enforcing the…

Imaging with hard x-rays is an invaluable tool in medicine, biology, materials science, and cultural heritage. Propagation-based x-ray phase-contrast imaging and tomography have been mostly used to resolve micrometer-scale structures inside…

Image and Video Processing · Electrical Eng. & Systems 2025-06-02 Margie P. Olbinado , David M. Paganin , Yin Cheng , Alexander Rack

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi

X-ray phase-contrast imaging enhances soft tissue visualization by leveraging the phase shift of X-rays passing through materials. It permits to minimize radiation exposure due to high contrast, as well as high resolution imaging limited by…

Optics · Physics 2024-03-13 Emilie Pietersoone , Jean Michel Létang , Simon Rit , Max Langer

A unified method for three-dimensional reconstruction of objects from transmission images collected at multiple illumination directions is described. The method may be applicable to experimental conditions relevant to absorption-based,…

Medical Physics · Physics 2022-12-07 Timur E. Gureyev , Hamish G. Brown , Harry M. Quiney , Leslie J. Allen

The ability to gain insights into the 3D properties of artificial or biological systems is often critical. However, 3D structures are difficult to retrieve at low dose and with extremely fast processing, as most techniques are based on…

Image and Video Processing · Electrical Eng. & Systems 2019-09-11 J. Duarte , R. Cassin , J. Huijts , M. Kholodtsova , B. Iwan , M. Kovacev , M. Fajardo , F. Fortuna , L. Delbecq , W. Boutu , H. Merdji

Multi-energy CT has long demonstrated its ability to enhance image quality with material decomposition. Yet, it has largely been limited to applications that already have high contrast. More recently, x-ray phase-contrast (XPC) imaging has…

Medical Physics · Physics 2025-08-19 Giavanna Jadick , Patrick La Rivière
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