Related papers: Label-free optical detection of single enzyme-reac…
Infrared spectroscopy is the technique of choice for chemical identification of biomolecules through their vibrational fingerprints. However, infrared light interacts poorly with nanometric size molecules. Here, we exploit the unique…
Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable…
Measuring the electrophoretic mobility of molecules is a powerful experimental approach for investigating biomolecular processes. A frequent challenge in the context of single-particle measurements is throughput, limiting the obtainable…
To monitor conformational changes of individual membrane transporters in liposomes in real time, we attach two fluorophores to selected domains of a protein. Sequential distance changes between the dyes are recorded and analyzed by Forster…
Recent progress in coherent quantum interactions between free-electron pulses and laser-induced near-field light have revolutionized electron wavepacket shaping. Building on these advancements, we numerically explore the potential of…
Multiplexed, real-time fluorescence detection at the single-molecule level is highly desirable to reveal the stoichiometry, dynamics, and interactions of individual molecular species within complex systems. However, traditionally…
Enzymes have been shown to diffuse faster in the presence of their reactants. Recently, we revealed new insights into this process of enhanced diffusion using single-particle tracking (SPT) with total internal reflection fluorescence (TIRF)…
A novel approach that allows access to long-sought information on the Hyperon-Nucleon (YN) interaction was developed by producing a hyperon beam within a few-body nuclear system, and studying final-state interactions. The determination of…
Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…
Biological electron transfer (ET) relies on quantum mechanical tunnelling through a dynamically folded protein. Yet, the spatiotemporal coupling between structural fluctuations and electron flux remains poorly understood, largely due to…
Accurate design of labelled oligo probes for the detection of miRNA biomarkers by Surface Enhanced Raman Scattering (SERS) may improve the exploitation of the plasmonic enhancement. This work, thus, critically investigates the role of probe…
Nanopore based sequencing has demonstrated significant potential for the development of fast, accurate, and cost-efficient fingerprinting techniques for next generation molecular detection and sequencing. We propose a specific multi-layered…
Imaging dynamical processes at interfaces and on the nanoscale is of great importance throughout science and technology. While light-optical imaging techniques often cannot provide the necessary spatial resolution, electron-optical…
Gene panel selection aims to identify the most informative genomic biomarkers in label-free genomic datasets. Traditional approaches, which rely on domain expertise, embedded machine learning models, or heuristic-based iterative…
Refractometric biosensing is a vital label-free tool for the real time detection and interaction analysis of biological and chemical substances. Nanophotonic platforms like Surface Plasmon Resonance (SPR) have played a critical role in…
We study the electrophoretic separation of polyelectrolytes of varying lengths by means of end-labeled free-solution electrophoresis (ELFSE). A coarse-grained molecular dynamics simulation model, using full electrostatic interactions and a…
A growing amount of evidence points to the fact that many enzymes exhibit fluctuations in their catalytic activity, which are associated with conformational changes on a broad range of timescales. The experimental study of this phenomenon,…
Selective spatial control of chemical reactions at the level of individual nanostructures remains a significant challenge. We introduce a light-activated system that combines plasmonic gold nanorods with a poly(N-isopropylacrylamide)…
Novelty detection is a critical task in various engineering fields. Numerous approaches to novelty detection rely on supervised or semi-supervised learning, which requires labelled datasets for training. However, acquiring labelled data,…
Raman spectroscopy is a powerful tool to detect, analyze and identify molecules. It has been a long-history pursuit to push the detection limit of Raman spectroscopy down to the fundamental single-molecule (SM) level. Due to the tiny cross…