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Laser-induced alignment of particles and molecules was long envisioned to support three-dimensional structure determination using "single-molecule diffraction" with x-ray free-electron lasers [PRL 92, 198102 (2004)]. However, the alignment…

Computational Physics · Physics 2025-02-05 Muhamed Amin , Jean-Michel Hartmann , Amit K. Samanta , Jochen Küpper

Sorting, filtering, moving and controlling colloidal particles is crucial in many fields, ranging from chemistry to biology and physics. Dielectrophoresis is an outstanding tool for the manipulation of small particles by AC electric fields,…

Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide for near-field illumination of unlabeled samples, thereby repositioning the highest spatial frequencies of the sample into…

Motivation: Cryo-Electron Tomography (cryo-ET) is a 3D bioimaging tool that visualizes the structural and spatial organization of macromolecules at a near-native state in single cells, which has broad applications in life science. However,…

Quantitative Methods · Quantitative Biology 2021-07-28 Xuefeng Du , Haohan Wang , Zhenxi Zhu , Xiangrui Zeng , Yi-Wei Chang , Jing Zhang , Min Xu

Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule F\"orster Resonance Energy Transfer (smFRET) is a powerful approach to inform on the structure and the dynamics of labeled molecules.…

By utilizing label distribution learning, a probability distribution is assigned for a facial image to express a compound emotion, which effectively improves the problem of label uncertainties and noises occurred in one-hot labels. In…

Computer Vision and Pattern Recognition · Computer Science 2021-07-26 Shasha Mao , Guanghui Shi , Licheng Jiao , Shuiping Gou , Yangyang Li , Lin Xiong , Boxin Shi

Low-loss electron energy loss spectroscopy (EELS) has emerged as a technique of choice for exploring the localization of plasmonic phenomena at the nanometer level, necessitating analysis of physical behaviors from 3D spectral data sets.…

Digitalization leads to data transparency for production systems that we can benefit from with data-driven analysis methods like neural networks. For example, automated anomaly detection enables saving resources and optimizing the…

Machine Learning · Computer Science 2021-06-23 Tom Hammerbacher , Markus Lange-Hegermann , Gorden Platz

Nonlinear optical methods, such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), are able to perform label free imaging, with chemical bonds specificity. Here, we demonstrate that the use of circularly…

Heterogeneous gas and solid catalyst reactions occur at the atomic level, and understanding and controlling complex catalytic reactions at this level is crucial for the development of improved processes and materials. There are postulations…

Chemical Physics · Physics 2017-05-19 Edward Boyes , Pratibha Gai

How atoms and electrons in a molecule move during a chemical reaction and how rapidly energy is transferred to or from the surroundings can be studied with flashes of laser light. However, despite prolonged efforts to develop various…

Chemical Physics · Physics 2022-01-05 JunWoo Kim , Jonggu Jeon , Tai Hyun Yoon , Minhaeng Cho

Enzymes are nano-scale machines that have evolved to drive chemical reactions out of equilibrium in the right place at the right time. Given the complexity and specificity of enzymatic function, bottom-up design of enzymes presents a…

Soft Condensed Matter · Physics 2025-05-28 Michalis Chatzittofi , Jaime Agudo-Canalejo , Ramin Golestanian

A key challenge for neuroscience is noninvasive, label-free sensing of action potential (AP) dynamics in whole organisms with single-neuron resolution. Here, we present a new approach to this problem: using nitrogen-vacancy (NV) quantum…

Quantum Physics · Physics 2016-12-26 J. F. Barry , M. J. Turner , J. M. Schloss , D. R. Glenn , Y. Song , M. D. Lukin , H. Park , R. L. Walsworth

Specific binding of proteins to DNA is one of the most common ways in which gene expression is controlled. Although general rules for the DNA-protein recognition can be derived, the ambiguous and complex nature of this mechanism precludes a…

Biomolecules · Quantitative Biology 2007-12-17 E. Moroni , M. Caselle , F. Fogolari

Several techniques have been employed for the direct visualization of cytoskeletal filaments and their associated proteins. Total-internal-reflection-fluorescence (TIRF) microscopy has a high signal-to-background ratio, but it suffers from…

Biological Physics · Physics 2022-01-21 Yazgan Tuna , Amer Al-Hiyasat , Jonathon Howard

DNA is now firmly established as a versatile and robust platform for achieving synthetic nanostructures. While the folding of single molecules into complex structures is routinely achieved through engineering basepair sequences, much less…

Soft Condensed Matter · Physics 2025-05-15 Soumen De Karmakar , Thomas Speck

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

Investigating nanoplasmonics using time-dependent approaches permits shedding light on the dynamic optical properties of plasmonic structures, which are intrinsically connected with their potential applications in photochemistry and…

Mesoscale and Nanoscale Physics · Physics 2024-06-18 Piero Lafiosca , Luca Nicoli , Silvio Pipolo , Stefano Corni , Tommaso Giovannini , Chiara Cappelli

We report DynaCLR, a self-supervised method for embedding cell and organelle Dynamics via Contrastive Learning of Representations of time-lapse images. DynaCLR integrates single-cell tracking and time-aware contrastive sampling to learn…

Label-free biosensors, including conventional quartz-crystal-microbalance (QCM) biosensor, are seriously affected by nonspecific adsorption of contaminants involved in analyte solution, and it is exceptionally difficult to extract the…

Biological Physics · Physics 2021-12-15 Kentaro Noi , Arihiro Iwata , Fumihito Kato , Hirotsugu Ogi
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