English
Related papers

Related papers: Label-free optical detection of single enzyme-reac…

200 papers

DNA nanotechnology uses predictable interactions of nucleic acids to precisely engineer complex nanostructures. Characterizing these self-assembled structures at the single-structure level is crucial for validating their design and…

Biological Physics · Physics 2025-10-21 Wangwei Dong , Zezhou Liu , Ruiyao Liu , Deborah Kuchnir Fygenson , Walter Reisner

Current single-molecule imaging techniques are incapable of providing stochastic information of individual chemical reactions. We have developed an optical imaging method for stochastic analysis of individual electrochemical reactions. The…

Optics · Physics 2023-05-22 Wei Liu , Meng Li , Houqiang Teng , Heshan Liu , Zhi Li , Yu Niu , Ziren Luo

Ion transport is a fundamental process in many physical, chemical, and biological phenomena, and especially in electrochemical energy conversion and storage. Despite its immense importance, demonstrations of label-free, spatially and…

Label-free identification and real-time tracking of biochemical substances became critical for molecular diagnostics and chemical analysis, yet conventional resonant terahertz metasurface sensing relies on a single resonance, limiting…

Electrochromic optical recording (ECORE) is a label-free method that utilizes electrochromism to optically detect electrical signals in biological cells with a high signal-to-noise ratio and is suitable for long-term recording. However,…

Coherent Raman scattering provides highly sensitive vibrational analysis through nonlinear light-matter interactions. However, its application to metal interfaces has remained challenging because the intrinsically large non-resonant…

Large amounts of labeled training data are one of the main contributors to the great success that deep models have achieved in the past. Label acquisition for tasks other than benchmarks can pose a challenge due to requirements of both…

Computer Vision and Pattern Recognition · Computer Science 2020-01-22 Clemens-Alexander Brust , Christoph Käding , Joachim Denzler

In the realm of healthcare, label-free sensing is a vital component for various applications. Micro-photonic technology presents a promising avenue to pursue pivotal goals. With the use of this technology, healthcare professionals and…

Optics · Physics 2024-04-24 Giuseppina Simone

A novel non-invasive microscopy technique for imaging and sizing of folded DNA molecules with the use of photovoltaic tweezers and phase-sensitive detection is elaborated and realized. This novel method is compared with the state-of-the-art…

Atomic Physics · Physics 2025-02-27 Lusine Tsarukyan , Anahit Badalyan , Rafael Drampyan

We present a protocol that detects molecular conformational changes with two nitroxide electron-spin labels and a nitrogen-vacancy (NV) center in diamond. More specifically, we demonstrate that the NV can detect energy shifts induced by the…

Quantum Physics · Physics 2022-12-21 C. Munuera-Javaloy , R. Puebla , B. D'Anjou , M. B. Plenio , J. Casanova

Label-free detecting multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics…

Quantitative Methods · Quantitative Biology 2023-08-14 Alexia Stollmann , Jose Garcia-Guirado , Jae-Sang Hong , Hyungsoon Im , Hakho Lee , Jaime Ortega Arroyo , Romain Quidant

A chemical kinetic model of the elongation dynamics of RNA polymerase along a DNA sequence is introduced. The proposed model governs the discrete movement of the RNA polymerase along a DNA template, with no consideration given to elastic…

Biomolecules · Quantitative Biology 2007-05-23 Yujiro Richard Yamada , Charles S. Peskin

Accurate detection and characterization of nanoparticles within confined spaces is crucial for applications ranging from nanofluidics to biotechnology. We present a novel approach that combines interferometric scattering (iSCAT) detection…

Label-free characterization of biological specimens seeks to supplement existing imaging techniques and avoid the need for contrast agents that can disturb the native state of living samples. Conventional label-free optical imaging…

Sensors that are able to detect and track single unlabelled biomolecules are an important tool both to understand biomolecular dynamics and interactions at nanoscale, and for medical diagnostics operating at their ultimate detection limits.…

Optics · Physics 2016-11-15 N. P. Mauranyapin , L. S. Madsen , M. A. Taylor , M. Waleed , W. P. Bowen

In combining DNA nanotechnology and high-bandwidth single-molecule detection in nanopipettes, we demonstrate an all-electric, label-free hybridisation sensor for short DNA sequences (< 100 nt). Such short fragments are known to occur as…

Solid-state nanopores are emerging platforms for single-molecule protein sequencing due to their tolerance to hash physiology environment and compatibility with different electrical and optical detection methods. However, they suffer from…

Applied Physics · Physics 2024-01-05 Yingqi Zhao , Aliaksandr Hubarevich , Jian-An Huang , Francesco De Angelis

Detecting chemical modifications on RNA molecules remains a key challenge in epitranscriptomics. Traditional reverse transcription-based sequencing methods introduce enzyme- and sequence-dependent biases and fragment RNA molecules,…

Cellular membrane dynamics play an important role in a variety of physiological processes. However, due to the stringent light-coupling conditions required for exciting evanescent waves, label-free mapping of cellular membrane dynamics on…

Biological Physics · Physics 2024-10-22 Chaoyang Gong , Yu-Cheng Chen

Attaching enzymes to nanostructures has proven useful to the study of enzyme functionality under controlled conditions and has led to new technologies. Often, the utility and interest of enzyme-tethered nanostructures lie in how the…