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Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this…

Chemical Physics · Physics 2011-01-18 Ilja Gerhardt , Lijian Mai , Antia Lamas-Linares , Christian Kurtsiefer

The characterization of individual nanoparticles in a liquid constitutes a critical challenge for environmental, material, and biological sciences. To detect nanoparticles, electronic approaches are especially desirable owing to their…

Single-molecule sensing is at the core of modern biophysics and nanoscale science, from revolutionizing healthcare through rapid, low-cost sequencing to understanding various physical, chemical, and biological processes at their most basic…

Mesoscale and Nanoscale Physics · Physics 2018-04-10 Daniel Gruss , Alex Smolyanitsky , Michael Zwolak

The ability to detect and identify molecules at high sensitivity without the use of labels or capture agents is important for medical diagnostics, threat identification, environmental monitoring, and basic science. Microtoroid optical…

Optics · Physics 2022-10-17 Gwangho Choi , Adley Gin , Judith Su

We present a new method for measuring low concentrations and simultaneously small volumes of fluorescent molecules based on the use of the Ebbesen effect of the extraordinary transmission (EOT) of light through an array of nano-holes. In…

Nanoparticles are ubiquitous, and methods that reveal insights into single-particle properties are highly desired to enable their advanced characterization. Techniques that achieve label-free single-nanoparticle detection often lack…

The clinical need for ultra-sensitive molecular analysis has motivated the development of several endpoint assay technologies capable of single molecule readout. These endpoint assays are now primarily limited by the affinity and…

Biological Physics · Physics 2019-05-22 Derin Sevenler , Jacob Trueb , M. Selim Ünlü

Single particle tracking systems monitor cellular processes with great accuracy in nano-biological systems. The emissions of the fluorescent molecules are detected with cameras or photodetectors. However, state-of-the-art imaging systems…

Quantitative Methods · Quantitative Biology 2018-05-16 Burhan Gulbahar , Gorkem Memisoglu

Single particle-resolved fluorescence imaging is an enabling technology in cold-atom physics. However, so far, this technique was not available for nanophotonic atom-light interfaces. Here, we image single atoms that are trapped and…

Optical-based nanothermometry represents a transformative approach for precise temperature measurements at the nanoscale, which finds versatile applications across biology, medicine, and electronics. The assembly of ratiometric fluorescent…

Optics · Physics 2025-04-01 Mahshid Iraniparast , Nishant Kumar , Igor Sokolov

Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and molecular composition are decisive for their functions, but simultaneous determination of both…

Biological Physics · Physics 2016-09-26 Stephan Block , Björn Johansson Fast , Anders Lundgren , Vladimir P. Zhdanov , Fredrik Höök

Single-cell analysis can unravel functional heterogeneity within cell populations otherwise obscured by ensemble measurements. However, non-invasive techniques that probe chemical entities and their dynamics are still lacking. This…

Applied Physics · Physics 2023-06-14 Nick Ruben Neuling , Robin Derek Allert , Dominik Benjamin Bucher

In a canonical optomechanical system, mechanical vibrations are dynamically encoded on an optical probe field which reciprocally exerts a backaction force. Due to the weak single photon coupling strength achieved with macroscopic…

Over the preceding years, nickel (Ni) and its compounds have been increasingly employed in various aspects of human social life, metallurgical/industrial manufactures, healthcare, and chemical processes. Although Ni is considered an…

Applied Physics · Physics 2025-07-08 Sudhanshu Naithani , Heena , Pooja Sharma , Samar Layek , Franck Thetiot , Tapas Goswami , Sushil Kumar

Placing a nanomechanical object in the evanescent near-field of a high-$Q$ optical microcavity gives access to strong gradient forces and quantum-noise-limited displacement readout, offering an attractive platform for precision sensing…

Label-free optical microscopy through absorption or scattering spectroscopy provides fundamental insights across biology and materials science, yet its sensitivity remains fundamentally limited by photon shot noise. While recent…

High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although…

Micro and nanoscale particles are crucial in various fields, from biomedical imaging to environmental processes. While conventional spectroscopy and microscopy methods for characterizing these particles often involve bulky equipment and…

Biological Physics · Physics 2024-11-26 Jie Liao , Maxwell Adolphson , Hangyue Li , Dipayon Kumar Sikder , Chenyang Lu , Lan Yang

The extraordinary sensitivity of plasmonic sensors is well known in the optics and photonics community. These sensors exploit simultaneously the enhancement and the localization of electromagnetic fields close to the interface between a…

Optical bio-markers have been used extensively for intracellular imaging with high spatial and temporal resolution. Extending the modality of these probes is a key driver in cell biology. In recent years, the NV centre in nanodiamond has…

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