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Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the…

Optics · Physics 2012-05-29 Chris J. Lee , Klaus J. Boller

In deep tissue photoacoustic imaging, the spatial resolution is inherently limited by acoustic diffraction. Moreover, as the ultrasound attenuation increases with frequency, resolution is often traded-off for penetration depth. Here we…

Diffraction limits the behaviour of light in optical systems and sets the smallest achievable line width at half the wavelength. With a novel subwavelength plasmonic lens to reduce the diffraction via an asymmetry and to generate and…

Optics · Physics 2009-01-30 K. R. Chen

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Far-field imaging beyond the diffraction limit is a long sought-after goal in various imaging applications, which requires usually an array of antennas or mechanical scanning. Here, we present an alternative and novel concept for this…

Optics · Physics 2014-06-10 Lianlin Li , Fang Li , Tiejun Cui , Yunhua Tan , Kan Yao

The optical resolution of a digital camera is one of its most crucial parameters with broad relevance for consumer electronics, surveillance systems, remote sensing, or medical imaging. However, resolution is physically limited by the…

Computer Vision and Pattern Recognition · Computer Science 2018-12-27 Thomas Köhler

In 2011, super-resolution imaging by microsphere superlens was emerged as a simple yet effective method to overcome the diffraction limit that limits the resolution of conventional lenses. Significant progress has since been made. Key…

Optics · Physics 2021-08-23 Zengbo Wang , Boris Luk'yanchuk , Limin Wu

Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…

Optics · Physics 2021-08-26 Zengbo Wang , Boris Luk'yanchuk

We consider passive imaging tasks involving discrimination between known candidate objects and investigate the best possible accuracy with which the correct object can be identified. We analytically compute quantum-limited error bounds for…

Quantum Physics · Physics 2022-10-26 Michael R Grace , Saikat Guha

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Super-resolution techniques overcome the diffraction-limit and get very high resolutions. A category of these techniques, e.g., STED achieves this by creating an illumination spot smaller than the Airy Disk. As a result, points are…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

The principles of difference imaging outlined and the technique of Alard and Lupton (1997) is generalised to generate the best possible difference images to within the limits of measurement error. It is shown how for a large database of…

Astrophysics · Physics 2007-05-23 Austin B. Tomaney

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

Our visual perception of our surroundings is ultimately limited by the diffraction limit, which stipulates that optical information smaller than roughly half the illumination wavelength is not retrievable. Over the past decades, many…

Spatial resolution of most imaging devices is fundamentally restricted by diffraction. This limitation is manifested in the loss of high spatial frequency information contained in evanescent waves. As a result, conventional far-field optics…

Optics · Physics 2010-03-16 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

The relentless pursuit of understanding matter at ever-finer scales has pushed optical microscopy to surpass the diffraction limit and produced the super-resolution microscopy which enables visualizing structures shorter than the wavelength…