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Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…

Optics · Physics 2023-08-02 Yilun Li , Fang Huang

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

In the ELTs era, where the need for versatile and innovative solutions to produce very high spatial resolution images has become a major issue, the search of synergies with other science fields seems a logic step. One of the considered…

Instrumentation and Methods for Astrophysics · Physics 2018-11-15 E. Quevedo , S. Velasco , C. Colodro-Conde , G. Rodríguez-Coira , A. Oscoz , R. L. López , J. Font , J. H. Brito , O. Llinas , S. Robaina , G. M. Callico , R. Sarmiento , R. Rebolo

In the last decade, metamaterials-based superlenses, with a resolution below Abbe's diffraction limit, have emerged. To obtain a rough estimate of the resolution of such superlenses, imaging of two subwavelength slits, separated by a…

Optics · Physics 2011-05-03 B. D. F. Casse , W. T. Lu , Y. J. Huang , S. Sridhar

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Far-field chemical microscopy providing molecular electronic or vibrational fingerprint information opens a new window for the study of three-dimensional biological, material, and chemical systems. Chemical microscopy provides a…

Optics · Physics 2023-06-06 Mingwei Tang , Yubing Han , Danchen Jia , Qing Yang , Ji-Xin Cheng

Superresolution fluorescence microscopy techniques beat the diffraction limit, enabling ultra-high resolution imaging in biological physics and nanoscience. In all cases that have been studied experimentally, the resolution scales inversely…

Optics · Physics 2012-10-10 Alexander Small

Despite all the success with developing super-resolution imaging techniques, the Abbe limit poses a severe fundamental restriction on the resolution of far-field imaging systems based on diffraction of light. Imaging with contact…

We propose and demonstrate a photon-efficient optical classifier to overcome the Rayleigh limit in spatial resolution. It utilizes mode-selective sum frequency generation and single-pixel photon detection to resolve closely spaced…

Quantum Physics · Physics 2020-10-21 He Zhang , Santosh Kumar , Yu-Ping Huang

The diffraction limited resolution of light focused by a lens was derived in 1873 by Ernst Abbe. Later in 1952, a method to reach sub-diffraction light spots was proposed by modulating the wavefront of the focused beam. In a related…

Optics · Physics 2018-03-20 Brijesh Kumar Singh , Harel Nagar , Yael Roichman , Ady Arie

Nonlinear optical microscopy provides elegant means for label-free imaging of biological samples and condensed matter systems. The widespread areas of application could even be increased if resolution was improved, which is currently…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

The resolution of optical imaging is classically limited by the width of the point-spread function, which in turn is determined by the Rayleigh length. Recently, spatial-mode demultiplexing (SPADE) has been proposed as a method to achieve…

Quantum Physics · Physics 2025-02-26 Giuseppe Buonaiuto , Cosmo Lupo

A new far-field optical microscopy technique capable of reaching nanometer-scale resolution has been developed recently using the in-plane image magnification by surface plasmon polaritons. This microscopy is based on the optical properties…

Strongly Correlated Electrons · Physics 2009-11-10 Igor I. Smolyaninov

Recent theoretical and experimental studies have shown that imaging with resolution well beyond the diffraction limit can be obtained with so-called superlenses. Images formed by such superlenses are, however, in the near field only, or a…

Optics · Physics 2009-11-11 Stephane Durant , Zhaowei Liu , Nicholas Fang , Xiang Zhang

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

Optics · Physics 2013-05-30 Osip Schwartz , Dan Oron

For more than a century, the diffraction limit has defined the resolution achievable by passive optical imaging systems. Although some resolution improvement can be gained through classical data processing of the image, it is limited by the…

Quantum Physics · Physics 2026-05-12 A. I. Lvovsky , Michael R. Grace , Saikat Guha , Mankei Tsang , Gerardo Adesso , Nicolas Treps