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Intrinsically disordered proteins (IDPs) are typically low in nonpolar/hydrophobic but relatively high in polar, charged, and aromatic amino acid compositions. Some IDPs undergo liquid-liquid phase separation in the aqueous milieu of the…

Biomolecules · Quantitative Biology 2017-05-19 Yi-Hsuan Lin , Jianhui Song , Julie D. Forman-Kay , Hue Sun Chan

Liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is a major undergirding factor in the regulated formation of membraneless organelles in the cell. The phase behavior of an IDP is sensitive to its amino acid…

Biomolecules · Quantitative Biology 2022-01-11 Yi-Hsuan Lin , Hue Sun Chan

Polyampholyte field theory and explicit-chain molecular dynamics models of sequence-specific phase separation of a system with two intrinsically disordered protein (IDP) species indicate consistently that a substantial polymer excluded…

Biomolecules · Quantitative Biology 2021-04-14 Tanmoy Pal , Jonas Wessén , Suman Das , Hue Sun Chan

Phase separation of intrinsically disordered proteins is important for the formation of membraneless organelles, or biomolecular condensates, which play key roles in the regulation of biochemical processes within cells. In this work, we…

Soft Condensed Matter · Physics 2020-03-18 Antonia Statt , Helena Casademunt , Clifford P. Brangwynne , Athanassios Z. Panagiotopoulos

Biomolecular condensates such as membraneless organelles, underpinned by liquid-liquid phase separation (LLPS), are important for physiological function, with electrostatics -- among other interaction types -- being a prominent force in…

Biomolecules · Quantitative Biology 2021-05-26 Jonas Wessén , Tanmoy Pal , Suman Das , Yi-Hsuan Lin , Hue Sun Chan

Biologically functional liquid-liquid phase separation of intrinsically disordered proteins (IDPs) is driven by interactions encoded by their amino acid sequences. Little is currently known about the molecular recognition mechanisms for…

Biomolecules · Quantitative Biology 2017-11-15 Yi-Hsuan Lin , Jacob P. Brady , Julie D. Forman-Kay , Hue Sun Chan

In view of recent intense experimental and theoretical interests in the biophysics of liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs), heteropolymer models with chain molecules configured as self-avoiding…

Biomolecules · Quantitative Biology 2018-08-30 Suman Das , Adam Eisen , Yi-Hsuan Lin , Hue Sun Chan

The sustenance of life depends on the high degree of organization that prevails through different levels of living organisms, from subcellular structures such as biomolecular complexes and organelles to tissues and organs. The physical…

Soft Condensed Matter · Physics 2019-01-14 Hanieh Falahati , Amir Haji-Akbari

Liquid-liquid phase separation is the mechanism underlying the formation of biomolecular condensates. Disordered protein regions often drive phase separation, but molecular interactions of disordered protein regions are not well understood,…

Biomolecules · Quantitative Biology 2020-08-10 Wade Borcherds , Anne Bremer , Madeleine B. Borgia , Tanja Mittag

The physical chemistry of liquid-liquid phase separation (LLPS) of polymer solutions bears directly on the assembly of biologically functional droplet-like bodies from proteins and nucleic acids. These biomolecular condensates include…

Biomolecules · Quantitative Biology 2020-01-31 Yi-Hsuan Lin , Jacob P. Brady , Hue Sun Chan , Kingshuk Ghosh

Phase separation of several different overall neutral polyampholyte species (with zero net charge) is studied in solution with two oppositely charged ion species that can form ion-pairs through an association reaction. A field theory…

Soft Condensed Matter · Physics 2023-03-28 Jonas Wessén , Tanmoy Pal , Hue Sun Chan

Intrinsically disordered proteins (IDPs) are important for biological functions. In contrast to folded proteins, molecular recognition among certain IDPs is "fuzzy" in that their binding and/or phase separation are stochastically governed…

Biomolecules · Quantitative Biology 2020-08-10 Alan N. Amin , Yi-Hsuan Lin , Suman Das , Hue Sun Chan

A new type of phase separation in the polyelectrolyte solutions consisting of several types of charged macromolecules differing in their degree of ionization is predicted via a general thermodynamic consideration. We show that even a small…

Soft Condensed Matter · Physics 2011-11-09 Igor Erukhimovich , Monica Olvera de la Cruz

A theory for sequence dependent liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs) in the study of biomolecular condensates is formulated by extending the random phase approximation (RPA) and field-theoretic…

Biomolecules · Quantitative Biology 2022-11-28 Jonas Wessén , Suman Das , Tanmoy Pal , Hue Sun Chan

Conformational properties of intrinsically disordered proteins (IDPs) are governed by a sequence-ensemble relationship. To differentiate the impact of sequence-local versus sequence-nonlocal features of an IDP's charge pattern on its…

Biomolecules · Quantitative Biology 2024-08-13 Tanmoy Pal , Jonas Wessén , Suman Das , Hue Sun Chan

Motivated by the idea that intrinsically disordered proteins (IDPs) condense into liquid-like droplets within cells, we carry out Monte Carlo simulations of a polymer lattice model to study the relationship between charge patterning and…

Soft Condensed Matter · Physics 2019-12-11 Nicholas A. S. Robichaud , Ivan Saika-Voivod , Stefan Wallin

In active systems, whose constituents have non-equilibrium dynamics at local level, fluid-fluid phase separation is widely observed. Examples include the formation of membraneless organelles within cells; the clustering of self-propelled…

Soft Condensed Matter · Physics 2025-07-11 M. E. Cates , C. Nardini

The effect of quenched disorder in the one-dimensional asymmetric exclusion process is reviewed. Both particlewise and sitewise disorder generically induces phase separation in a range of densities. In the particlewise case the existence of…

Statistical Mechanics · Physics 2011-05-20 Joachim Krug

Phase separation in salt-free symmetric mixtures of oppositely charged rodlike polyelectrolytes is studied using quasi-analytical calculations. Stability analyses for the isotropic-isotropic and the isotropic-nematic phase transitions in…

Soft Condensed Matter · Physics 2010-07-20 Rajeev Kumar , Debra Audus , Glenn H. Fredrickson

Liquid-liquid phase separation has emerged as one of the important paradigms in the chemical physics as well as biophysics of charged macromolecular systems. We elucidate an equilibrium phase separation mechanism based on charge regulation,…

Soft Condensed Matter · Physics 2020-12-25 Arghya Majee , Markus Bier , Ralf Blossey , Rudolf Podgornik
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