Related papers: Single-Molecule Protein Identification by Sub-Nano…

Metal nanoantennas enable the manipulation of light emission and detection at the single photon level by confining light into very small volumes. Emitters coupled to these plasmonic structures are thus ideal candidates for usage in quantum…

Nuclear magnetic resonance (NMR) spectroscopy is one of the leading techniques for protein studies. The method features a number of properties, allowing to explain macromolecular interactions mechanistically and resolve structures with…

Multispectral imaging (MSI) captures data across multiple spectral bands, offering enhanced informational depth compared to standard RGB imaging and benefiting diverse fields such as agriculture, medical diagnostics, and industrial…

Single-molecule fluorescence techniques have revolutionized our ability to study proteins. However, the presence of a fluorescent label can alter the protein structure and/or modify its reaction with other species. To avoid the need for a…

Optical fiber sensing carries a number of potential advantages for diagnostics and biomarker detection and monitoring, yet particular challenges persist in linking molecular recognition events to a change in the refractive index. DNA…

Protein function and dynamics are closely related to its sequence and structure. However prediction of protein function and dynamics from its sequence and structure is still a fundamental challenge in molecular biology. Protein…

Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules. This larger number of molecules and the associated larger dynamic range have major implications in the development of proteomics technologies. We examine…

Cellular heterogeneity is important to biological processes, including cancer and development. However, proteome heterogeneity is largely unexplored because of the limitations of existing methods for quantifying protein levels in single…

Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…

Single-photon detectors based on superconducting nanowires (SSPDs or SNSPDs) have rapidly emerged as a highly promising photon-counting technology for infrared wavelengths. These devices offer high efficiency, low dark counts and excellent…

Accurately assigning folds for divergent protein sequences is a major obstacle to structural studies and underlies the inverse protein folding problem. Herein, we outline our theories for fold-recognition in the "twilight-zone" of sequence…

Analyzing proteins from single cells by tandem mass spectrometry (MS) has become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and…

Single-photon detectors (SPDs) at near infrared wavelengths with high system detection efficiency (> 90%), low dark count rate (< 1 counts per second, cps), low timing jitter (< 100 ps), and short reset time (< 100 ns) would enable landmark…

Discriminating the low-abundance hydroxylated proline from hydroxylated proline is crucial for monitoring diseases and eval-uating therapeutic outcomes that require single-molecule sensors. While the plasmonic nanopore sensor can detect the…

Single-molecule surface-enhanced Raman spectroscopy based on a particle trapped in a plasmonic nanopores provides a unique method for continued and controlled detection of peptide and DNA oligonucleotides in liquid medium. However, the…

Ultrafast, high quantum efficiency single photon detectors are among the most sought-after elements in modern quantum optics and quantum communication. High photon detection efficiency is essential for scalable measurement-based quantum…

Post-translational modifications (PTMs) play essential roles in regulating protein structure, function, and cellular signalling. However, peptide level discrimination of hydroxylation at the single-molecule level remains difficult. Here, we…

Single-molecule tracking is a powerful way to look at the dynamic organization of plasma membranes. However, there are some limitations to its use. For example, it was recently observed, using numerical simulation, that time-averaging…

Computational spectrometers are pivotal in enabling low-cost, in-situ and rapid spectral analysis, with potential applications in chemistry, biology, and environmental science. However, filter-based spectral encoding approaches typically…

Surface plasmon resonances (SPRs) are crucial for confining light beyond the diffraction limit, yet heavy metal losses often limit their spectral localization. Here, we propose a practical strategy for enabling the spectral localization of…